LOCUS       sequence-272035-       10323 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Communication-activated luciferase in Neurospora crassa.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10323)
  AUTHORS   Fischer MS, Jonkers W, Glass NL
  TITLE     Integration of Self and Non-self Recognition Modulates Asexual 
            Cell-to-Cell Communication in Neurospora crassa.
  JOURNAL   Genetics. 2019 Apr;211(4):1255-1267. doi: 
            10.1534/genetics.118.301780. Epub 2019 Feb 4.
  PUBMED    30718271
REFERENCE   2  (bases 1 to 10323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.118.301780"; journalName: "Genetics"; date: 
            "2019-04"; volume: "211"; issue: "4"; pages: "1255-1267"
COMMENT     Sequence Label: Pprm1-luciferase; his-3
FEATURES             Location/Qualifiers
     source          1..10323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        86..104
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3675..3691)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3676..3692)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(3721..3739)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(6400..6418)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6401..6418)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6499..6518)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6618..6640
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6678..6696)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6764..6868
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6869..6937,6938..7729)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7087..7106)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7900..8488
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8389..8408
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8642..8659
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        8746..8764
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             9039..9050
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tgatgtttca aacttgattg acagcgaacg aaacccctga aacccgttat agatatgtat
       61 agatttgacg gggtagcttg gcccttaata cgactcacta tagggcgaat tggagctcca
      121 ccgcggtggc ggccgctcca tcttcctcct ggtcgcatac ttcatgacag cacggcgatg
      181 ttcaataaca gcgaagtgaa ggggacgccg gcggaggtga ggtgaagatg gtgaagacgt
      241 cggggcctct cacgtgcctc tgacgcacgg atcatcactc tgcctgccgc ttcaacaagt
      301 acctatgaat ggacatggcc aaatgcaaat ccccatggac caaacaacaa gcggcggccg
      361 tcgactgttg aatgaacgcg gcaatccatt tttctgcgtg agtcatttga acggattcgt
      421 cattcagagt ctttatttaa catttttgaa acagcagaat ggaagcgccc ggctgctggc
      481 ctctccatct ctccccggct ggggcttgtc gggtcagttg tcgtcattgg gctttcagag
      541 gctccggggc attcactggc tgtcagggct ccagcgggta gtggccccac tttccacttg
      601 gcatcggcag aagtggaagc gcgtctgttg gacccttgtc aggcaccaat cactggccgg
      661 agacagcccg cggttgaaac gcaggaacgc cttccacttt tgagtgccac cttctttttt
      721 ttttcttcct ctttttcttc ctcttttcca catcccggca ctcattgcat ttcagcatcg
      781 gaactaaaca ccaggatgag ctgaggccag atctggcgac cggcctcgat tgcaacccaa
      841 catctgctct gccacagact ccagatcaga gaggctgatg gtggtgaagg acatcacggt
      901 gacccattgt caaaggtgga atacaggacc ggcacacatc gttcaccttg ccgtgagtat
      961 gatgatgagc cctcccgagt cccatcaggt ggccttgtct catgaacaca cagcatggcc
     1021 cggctgcaag cgcgccagac atcaagacga caggctcaca ttgatccatc aacccagcca
     1081 gtttttgatg ggctgcctct ccaccaagga aatccggggt gttcaaggat caatccatga
     1141 caaagaacga ctggctgcca gagtccgggc gcttagacaa ccaccgcggc tacgggacga
     1201 ctaccccttg atcagcctcc tcccactcca ctcactgttc acgcacacag attcaaacaa
     1261 aggcaatgtg ctttcaagaa acaatgggcc ttcttcctat tctgggaccc ggtgcgccta
     1321 ttgtcatttg cacatcacct gctatcccat cttttggtcc ttcatcccaa ctccggtccc
     1381 aggtctcaac ttattcactg atccaccagc ctccgggagt ccgggccagt caagtcacta
     1441 gcctgcctgg gtccgtctta ttctcccgtc cgtcgtctta ttgacgcact ttctttctat
     1501 tgttcactca cctcctcacc tgttcgtttc ccattcttca cctattcata tctccagtat
     1561 gcagtcatag tgggcaagcc tttctgattt ggataccctc ttgtttcctt tgactcactt
     1621 ctcttgttcc tttctcttgc tcatttgcat gtcctttttt cctttttccc ggtcttcggc
     1681 tcagtaattc tttctcgaga gtgttcatcc agctggtcta gacgagtgaa cagcagtttt
     1741 ctgtcttttt ttttctccta gcagacatac accacgcgct ctcacggcat tcgcttgact
     1801 tttcactttt cgacttttgc acgattagta gacaactaca cgatatacta caccatcttg
     1861 ctggttcaag aataagaacg atatatcaaa attggatccc cgggttaatt aacatggagg
     1921 acgccaagaa catcaagaag ggccccgccc ccttctaccc cctcgaggac ggcaccgccg
     1981 gcgagcagct ccacaaggcc atgaagcgct acgccctcgt ccccggcacc atcgccttcg
     2041 taggtttcct ccagctctcg cctccagcac ccgaggcaca tctcgggcat cttcacaaca
     2101 acagacactg acatctcatt ctcacagacc gacgcccaca tcgaggtcaa catcacctac
     2161 gccgagtact tcgagatgtc cgtccgcctc gccgaggcca tgaagcgcta cggcctcaac
     2221 accaaccacc gcatcgtcgt ctgctccgag aactccctcc agttcttcat gcccgtcctc
     2281 ggcgccctct tcatcggcgt cgccgtcgcc cccgccaacg acatctacaa cgagcgcgag
     2341 ctcctcaact ccatgaacat ctcccagccc accgtcgtct tcgtctccaa gaagggcctc
     2401 cagaagatcc tcaacgtcca gaagaagctc cccatcatcc agaagatcat catcatggac
     2461 tccaagaccg actaccaggg cttccagtcc atgtacacct tcgtcacctc ccacctcccc
     2521 cccggcttca acgagtacga cttcgtcccc gagtccttcg accgcgacaa gaccatcgcc
     2581 ctcatcatga actcctccgg ctccaccggc ctccccaagg gcgtcgccct cccccaccgc
     2641 accgcctgcg tccgcttctc ccacgcccgc gaccccatct tcggcaacca gatcatcccc
     2701 gacaccgcca tcctctccgt cgtccccttc caccacggct tcggcatgtt caccaccctc
     2761 ggctacctca tctgcggctt ccgcgtcgtc ctcatgtacc gcttcgagga ggagctcttc
     2821 ctccgctccc tccaggacta caagatccag tccgccctcc tcgtccccac cctcttctcc
     2881 ttcttcgcca agtccaccct catcgacaag tacgacctct ccaacctcca cgagatcgcc
     2941 tccggcggcg cccccctctc caaggaggtc ggcgaggccg tcgccaagcg cttccacctc
     3001 cccggcatcc gccagggcta cggcctcacc gagaccacct ccgccatcct catcaccccc
     3061 gagggcgacg acaagcccgg cgccgtcggc aaggtcgtcc ccttcttcga ggccaaggtc
     3121 gtcgacctcg acaccggcaa gaccctcggc gtcaaccagc gcggcgagct ctgcgtccgc
     3181 ggccccatga tcatgtccgg ctacgtcaac aaccccgagg ccaccaacgc cctcatcgac
     3241 aaggacggct ggctccactc cggcgacatc gcctactggg acgaggacga gcacttcttc
     3301 atcgtcgacc gcctcaagtc cctcatcaag tacaagggct accaggtcgc ccccgccgag
     3361 ctcgagtcca tcctcctcca gcaccccaac atcttcgacg ccggcgtcgc cggcctcccc
     3421 gacgacgacg ccggcgagct ccccgccgcc gtcgtcgtcc tcgagcacgg caagaccatg
     3481 accgagaagg agatcgtcga ctacgtcgcc tcccaggtca ccaccgccaa gaagctccgc
     3541 ggcggcgtcg tcttcgtcga cgaggtcccc aagggcctca ccggcaagct cgacgcccgc
     3601 aagatccgcg agatcctcat caaggccaag aagggcggca agtccaagct ctgagaattc
     3661 gatatcaagc ttatcgatac cgtcgacctc gagggggggc ccggtaccca gcttttgttc
     3721 cctttagtga gggttaatta gggcgtggac ggctaatggg gtctgaatgc taaaagacac
     3781 catttcccac actccctctg atttttttta tggttttttt ttatcttcat ttttcgccgt
     3841 cttttattcg gttgtttggg aggttcacat gcaacgtgca agactgcaag ttttttgaga
     3901 tgggcgcttc atgcaatgca gcatcactgc agggacattg tgacatccac gtttcgatcg
     3961 tctgtgcttc ccccgttcat gtacgcatga tctgaaaaaa aaaaaaaaaa aaaaggttga
     4021 tgtaataatg aaaataaaca cccagggacg agcacctcat ctgctggaat agggagaaaa
     4081 actcaagggc cgtttgggtc tccacactta ccaacagatt catgtactga tcttcagcct
     4141 gtacagaaca gtacaatgtc caaaactcgt tatcattact ctatgtgccc aaaatgaccc
     4201 agatatacat caacccacta catccttttg ttcttgtttt tttttttttt ttttgctact
     4261 tcgaaaagaa tttccgttta gttgctcttg ctcatgtgct caagacggat gctgaccgcc
     4321 cttctgtgag cctcgagctc ctcaacctta gccaactgca tgacagcctg gccgacgttt
     4381 ttgagaccct cggcagtcaa gttggagctg gtaatgtgct tgacgaacga ggcgaaattg
     4441 acgccagagt actgcttgcc aaagccatag gtagctatgt gtcttgacgt tagcagaaga
     4501 atctgtgaag cggggctatt tatgatatgt aacttacgca gcgagtggtt aacaccagca
     4561 gagtaatcgc caacggactc aggagtccag gcgccaatga agacactacc ggcgttcatg
     4621 acaagatcga cagccttctc ggcctccttg atctggagga tcaagtgctc aggagcgtac
     4681 ttgttgctga gctccatggc ctcctcgacg gtcttgacct gcacggtgat cgagtgggcg
     4741 atggagccac ggacaatctg gacgcgagga agctccgtag cctgacggtg aacctcgtcc
     4801 tcaatagcct gaagatgctc ctcgtcgagg tcaatagcga tcaggatgac ctgactgtca
     4861 acgccgtgct cagcctggga caggagatcc gaggcaacga acgcggggtt ggcgtccttg
     4921 tcagcgatga ccagcacctc ggacgggcca gcgggcatgt caataccaac ggcagcgttg
     4981 gtgtcgttgc tgacgaacat cttggcagca gtgacgaact ggttaccggg gccgagaatc
     5041 ttgtcgacct tggtgatgct ctcggtgccg taggccatgg cagctacggc ctgggcaccg
     5101 ccggcaagca cgatggactc ggccccaacc ttgtgagcga cgtggacaat ctcgggagtg
     5161 atggttccgt cggcgcgggg aggagaggcg aacacaatct tgttgcagcc ggcgaccatg
     5221 gcgggaacac ccagcataag ggcagtgctg gggagaacgg cggtaccgcc ggggatgtag
     5281 cagccgacgg cctcgatggg acgagagaaa cggctgcaga caacaccggg catggtctcg
     5341 acctggaggg gcttctcctc cttctgggcg gcgtggaact tgcggatgtt ctcgaaggac
     5401 acgtcgatgg cagcaatggt ctcctcaggg agctgcataa gctccttggg gaagggcgcc
     5461 ttcaggacgg ggctagtaag agaggtagcc ttctcgaact tgtgagtgta cgacagaaca
     5521 gccttgtcgc cgttcttgcg gacgtcctcg atgatgggga caatgatctt gtagatggca
     5581 tcggacgact tttgcgcagg acgcttgaga gcagcatcga gctcctcggt agagaccttg
     5641 gaggcgtcga aacgtctcat ggtgatcttc tcaggggtgg tctcctgggc ggcctccttg
     5701 gtgacagggg ccgaagtggc agcgagagcg gacgccgcag gcttgatgcc ctccttctca
     5761 gcccacttac ccttagcatc tccagtcctg cgcttgacct tccagctctt ggcgtcaagg
     5821 ctcctttcga tatcggcaag agtaacgccg gcagcaacgg ccctggtaag agcaaagtag
     5881 aagagatcgg cagcctcaaa ggcgatttcc tggggggtct gagcggtgca gagctcctca
     5941 gcctcctcca tgatcttggc ccggactagc ttctcatcgg agaagagacg ggcagtgtag
     6001 gagccctcgg gggcagactg tttcctcgaa atcaaagtct gctcgagctt gggaaggcct
     6061 ttgagctgac caaagcagcc ggactgatcg aggtggcaga aacgaccctt ctgcttcacg
     6121 acaaacttga gagcatcgtt atcgcagtca agcgagatgc ggacgagctc ctgagtgtct
     6181 ccggaagtag cacccttgta ccagagaccg cgcttccggc tctgatagac accagtctgt
     6241 gtcctgaggg cctcgttcac actctcggca ctgctgtaga ccagacccag agcagtgtcg
     6301 tgctcatcaa cgacaacggt ggggagaaga ccatcgggac ggtcagactt ccagacgctc
     6361 gagaggatgg tggagatggc aagcttcagc tgctcgagtt ctatagtgtc acctaaatcg
     6421 tatgtgtatg atacataagg ttatgtatta attgtagccg cgttctaacg acaatatgtc
     6481 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     6541 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     6601 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     6661 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     6721 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     6781 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     6841 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     6901 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     6961 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     7021 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     7081 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     7141 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     7201 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     7261 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     7321 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     7381 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     7441 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     7501 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     7561 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     7621 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     7681 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     7741 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     7801 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     7861 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     7921 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     7981 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     8041 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     8101 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     8161 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     8221 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     8281 acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     8341 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     8401 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     8461 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     8521 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     8581 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     8641 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     8701 gcgttggccg attcattaat gcaggttaac ctggcttatc gaaattaata cgactcacta
     8761 tagggagacc ggcagatcca atgcggatgg attcgctcac tcggtgccgc ggcggtgcga
     8821 agttcctgcc gtggattggg ggattgcaca gacggtggag tgcttgctac agctaggtac
     8881 ccagttgcac accaaacggt gcccctgtgc ccctgcgtct aggtgtgtcc tgtgagcaac
     8941 tgcgcccgcg cgcggcaccg ggaagctgcg ggaacctcaa tggatgattg gcgccagatc
     9001 cccggcatca cctcagcaca tgggcagccc gagaaggaat cgagggtaga agatattcca
     9061 gggtttccgc tagcacaact tccaagcgcc tctaccgcta ctccacacac ccacgctgaa
     9121 tggcacaacc actgtaggcc tagcggtccc agctccccgt aaccacgcag ccccagcgac
     9181 cgaggtcatc atgacactat gacgacaatc tggcgggaga cttgggaaaa tcgatgacgg
     9241 gcacctacct tgggaacaat agagcaagtc cagtcaacgc cctagggaag ggggaaaaaa
     9301 aaaaagagcc cattctcaca aggagcaaca ctgtgctgct atcatagtac actacaagca
     9361 aagtcgggat gttgctagaa tccgtcggct acgtcggtag atacctccca taggcagtaa
     9421 gtctaggtat ccaatttata gttccgcgta tttcttcgtc gaacggggca cagcaaagcc
     9481 taacagccgc agtgcctcgc aaccaacacc aagcagtaat ctcatccatc catacagatc
     9541 ctcccacaat atctttacat gcgcacttgc gctgtgttag cagtttaggt tccactacca
     9601 aggctgacac gagcagcact gacagcaaac tgctgcttgg ggccaagaaa ttggcagttt
     9661 gctgagcacc tacatatcag tacacatcaa ccctggttta attgttagcc tggtactgtt
     9721 aaccgtcatg aacgaggacg tggaggaaga accacgctaa caattgaacc agggttgatg
     9781 tatacctctc ttaccatatt tgcttgcttc cacgtcccta tcctcaagcg attttgttct
     9841 tgactttttg tatttcaacg caaaggccat tcaggtttct catgttcaac cctttggatg
     9901 gcgatgccgt tcgtcccagg acctcgtgac ctttgaaaga cgagaaatat atcccaaagc
     9961 aacttctgct tatggacaag caactgcatg gcggcccaca actcatatct cgcgtaatca
    10021 tccaaacgct aggcccgagt actctactgt tctcgagaca cctaccataa tatgtaccca
    10081 atcagacatg acaacagtca accaaacata ccctgatccg gccaaaccct gacatcccta
    10141 gatgatggtt aggcacacaa atggaaaacg ggactcgttg gttaggtggg aacgcttgta
    10201 ttgcagccag ccaaccgact ctcctcgtcc ctctcgagtc ccgttattgc cgtttgaccc
    10261 agccgcccag ccgccaaaca gctgagggag ccaatggtct aatacaccgt ccaacggccc
    10321 cag
//
