LOCUS       sequence-272036-       12284 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses epitope-tagged human TET3 V908L variant in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12284)
  AUTHORS   Beck DB, Petracovici A, He C, Moore HW, Louie RJ, Ansar M, Douzgou 
            S, Sithambaram S, Cottrell T, Santos-Cortez RLP, Prijoles EJ, Bend 
            R, Keren B, Mignot C, Nougues MC, Ounap K, Reimand T, Pajusalu S, 
            Zahid M, Saqib MAN, Buratti J, Seaby EG, McWalter K, Telegrafi A, 
            Baldridge D, Shinawi M, Leal SM, Schaefer GB, Stevenson RE, Banka S,
            Bonasio R, Fahrner JA
  TITLE     Delineation of a Human Mendelian Disorder of the DNA Demethylation 
            Machinery: TET3 Deficiency.
  JOURNAL   Am J Hum Genet. 2020 Jan 3. pii: S0002-9297(19)30471-9. doi: 
            10.1016/j.ajhg.2019.12.007.
  PUBMED    31928709
REFERENCE   2  (bases 1 to 12284)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Am J Hum 
            Genet. 2020 Jan 3. pii: S0002-9297(19)30471-9. doi: 
            10.1016/j.ajhg.2019.12.007."
COMMENT     Sequence Label: pINTO-N3::hTET3 V908L
FEATURES             Location/Qualifiers
     source          1..12284
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    430..551
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(467..486)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     521..540
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(600..616)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(600..616)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(613..635)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    624..640
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(648..654,655..672,673..678))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    693..714
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1463..1480)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1634..2222)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1714..1733)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2393..3184,3185..3253))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3016..3035
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3254..3358)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3433..3452)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4604..4983
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4984..5187
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5138..5158
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    5189..5207
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5210..5228
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     5238..5262
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5350..5359
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5359..5382
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5392..5418
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(5428..5433,5434..5457,5458..5493,5494..5517)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             5527..5547
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    8353..8364
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     primer_bind     complement(11049..11066)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11055..11279
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11325..11753
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11412..11431)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11622..11643
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11762..11782)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11767..12096
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11947..12082
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     12009..12028
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        12144..12191
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             join(12210..12284,1..300)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
ORIGIN
        1 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac
       61 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg
      121 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac
      181 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag
      241 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga
      301 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
      361 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
      421 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
      481 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
      541 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
      601 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
      661 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
      721 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
      781 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgctag tctgcaatga
      841 gttcgagttg gcgcgcctgt cattctaaat ctctctttca gcctaaagct ttttccccgt
      901 atccccccag gtgtctgcag gctcaaagag cagcgagaag cgttcagagg aaagcgatcc
      961 cgtgccacct tccccgtgcc cgggctgtcc ccgcacgctg ccggctcggg gatgcggggg
     1021 gagcgccgga ccggagcgga gccccgggcg gctcgctgct gccccctagc gggggaggga
     1081 cgtaattaca tccctggggg ctttgggagg gggctgtccc cgtgagctcc caggcgcgcc
     1141 tgtcattcta aatctctctt tcagcctaaa gctttttccc cgtatccccc caggtgtctg
     1201 caggctcaaa gagcagcgag aagcgttcag aggaaagcga tcccgtgcca ccttccccgt
     1261 gcccgggctg tccccgcacg ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc
     1321 ggagccccgg gcggctcgct gctgccccct agcgggggag ggacgtaatt acatccctgg
     1381 gggctttggg ggggggctgt ccccgtgagc tcccaggcgc gccaactcgg tactcgggga
     1441 tttaattaag ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     1501 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     1561 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     1621 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     1681 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     1741 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     1801 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     1861 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     1921 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     1981 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     2041 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     2101 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     2161 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     2221 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     2281 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     2341 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     2401 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     2461 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     2521 caatgatacc gcgagaacca cgctcaccgg ctccagattt atcagcaata aaccagccag
     2581 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     2641 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     2701 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     2761 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     2821 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     2881 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     2941 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     3001 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     3061 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     3121 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     3181 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     3241 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     3301 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     3361 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
     3421 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
     3481 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
     3541 cttgaccgac aattgttaat taagctagca gtctgcaatg agttcgagtt ggcgcgcctg
     3601 tcattctaaa tctctctttc agcctaaagc tttttccccg tatcccccca ggtgtctgca
     3661 ggctcaaaga gcagcgagaa gcgttcagag gaaagcgatc ccgtgccacc ttccccgtgc
     3721 ccgggctgtc cccgcacgct gccggctcgg ggatgcgggg ggagcgccgg accggagcgg
     3781 agccccgggc ggctcgctgc tgccccctag cgggggaggg acgtaattac atccctgggg
     3841 gctttgggag ggggctgtcc ccgtgagctc ccaggcgcgc ctgtcattct aaatctctct
     3901 ttcagcctaa agctttttcc ccgtatcccc ccaggtgtct gcaggctcaa agagcagcga
     3961 gaagcgttca gaggaaagcg atcccgtgcc accttccccg tgcccgggct gtccccgcac
     4021 gctgccggct cggggatgcg gggggagcgc cggaccggag cggagccccg ggcggctcgc
     4081 tgctgccccc tagcggggga gggacgtaat tacatccctg ggggctttgg gggggggctg
     4141 tccccgtgag ctcccaggcg cgccaactcg gtactcgggg atgctagcgc accatcaacg
     4201 aggaggacat cttggctaac caacccctgt tgttacagtc catccaggac agacttggct
     4261 cgctggtggg ccaggacagc gggtatgtgg ggggtcttcc caagaacgtt aaggaaaagc
     4321 tgctgagctt gaagacttta caaagcgagc tattcgaagt tgaaaaggaa tttcaggtgg
     4381 agatgtttga gctagagaac aagtttctgc agaagtacaa gcccatttgg gagcagcggt
     4441 ccaggatcat ttcaggacaa gagcaaccca agccggaaca gatcgcaaag ggccaagaga
     4501 ttgtggagtc actcaatgag acggagttgc aattgcatga agaatctgct tagggttagg
     4561 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact
     4621 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     4681 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     4741 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     4801 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     4861 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     4921 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     4981 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     5041 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     5101 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     5161 cggtgggagg tctatataag cagagctctc cctatcagtg atagagatct ccctatcagt
     5221 gatagagatc gtcgacgagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca
     5281 cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccggac tctagcgttt
     5341 aaacttaagg ccaccatgga ctacaaggat gacgacgata agggcagcgg atacccatac
     5401 gacgtgcctg actacgccgg cagcgctagc gcatggtccc atcctcaatt cgagaaaggc
     5461 ggcggatctg gcggtggaag cggaggctcc gcctggagcc accctcagtt cgaaaagtcc
     5521 ggcagcgaga acctgtactt ccagggctcc accggtagcg gtaccgagct cggatccgat
     5581 ggaggactcg acagccagtt tcaggtgccc ctggccgtcc agccggacct gccaggcctt
     5641 tatgacttcc ctcagcgcca ggtgatggta gggagcttcc cggggtctgg gctctccatg
     5701 gctgggagtg agtcccaact ccgagggggt ggagatggtc gaaagaaacg gaaacggtgt
     5761 ggtacttgtg agccctgccg gcggctggaa aactgtggcg cttgcactag ctgtaccaac
     5821 cgccgcacgc accagatctg caaactgcga aaatgtgagg tgctgaagaa aaaagtaggg
     5881 cttctcaagg aggtggaaat aaaggctggt gaaggagccg ggccgtgggg acaaggagcg
     5941 gctgtcaaga caggctcaga gctcagccca gttgatggac ctgttccagg tcagatggac
     6001 tcagggccag tgtaccatgg ggactcacgg cagctaagcg cctcaggggt gccggtcaat
     6061 ggtgctagag agcccgctgg acccagtctg ctggggactg ggggtccttg gcgggtagac
     6121 caaaagcccg actgggaggc tgccccaggc ccagctcata ctgctcgcct ggaagatgcc
     6181 cacgatctgg tggccttttc ggctgtggcc gaagctgtgt cctcttatgg ggcccttagc
     6241 acccggctct atgaaacctt caaccgtgag atgagtcgtg aggctgggaa caacagcagg
     6301 ggaccccggc cagggcctga gggctgctct gctggcagcg aagaccttga cacactgcag
     6361 acggccctgg ccctcgcgcg gcatggtatg aaaccaccca actgcaactg cgatggccca
     6421 gaatgccctg actacctcga gtggctggag gggaagatca agtctgtggt catggaagga
     6481 ggggaggagc ggcccaggct cccagggcct ctgcctcctg gtgaggccgg cctcccagca
     6541 ccaagcacca ggccactcct cagctcagag gtgccccaga tctctcccca agagggcctg
     6601 cccctgtccc agagtgccct gagcattgcc aaggaaaaaa acatcagctt gcagaccgcc
     6661 attgccattg aggccctcac acagctctcc tctgccctcc cgcagccttc tcattccacc
     6721 ccccaggctt cttgccccct tcctgaggcc ttgtcacctc ctgccccttt cagatctccc
     6781 cagtcttacc tccgggctcc ctcatggcct gtggttcctc ctgaagagca ctcatctttt
     6841 gctcctgata gctctgcctt ccctccagca actcctagaa ctgagttccc tgaagcctgg
     6901 ggcactgaca cccctccagc aacgccccgg agctcctggc ccatgcctcg cccaagcccc
     6961 gatcccatgg ctgaactgga gcagttgttg ggcagcgcca gtgattacat ccagtcagta
     7021 ttcaagcggc ctgaggccct gcctaccaag cccaaggtca aggtggaggc accctcttcc
     7081 tccccggccc cggccccatc ccctgtactt cagagggagg ctcccacgcc atcctcggag
     7141 cccgacaccc accagaaggc ccagaccgcc ctgcagcagc acctccacca caagcgcagc
     7201 ctcttcctag aacaggtgca cgacacctcc ttccctgctc cttcagagcc ttctgctcct
     7261 ggctggtggc ccccaccaag ttcacctgtc ccacggcttc cagacagacc acccaaggag
     7321 aagaagaaga agctcccaac accagctgga ggtcccgtgg gaacggagaa agctgcccct
     7381 gggatcaagc ccagtgtccg aaagcccatt cagatcaaga agtccaggcc ccgggaagca
     7441 cagcccctct tcccacctgt ccgacagatt gtcctggaag ggcttaggtc cccagcctcc
     7501 caggaagtgc aggctcatcc accggcccct ctgcctgcct cacagggctc tgctgtgccc
     7561 ctgcccccag aaccttctct tgcgctattt gcacctagtc cctccaggga cagcctgctg
     7621 ccccctactc aggaaatgag gtcccccagc cccatgacag ccttgcagcc aggctccact
     7681 ggccctcttc cccctgccga tgacaagctg gaagagctca tccggcagtt tgaggctgaa
     7741 tttggagata gctttgggct tcccggcccc ccttctgtgc ccattcagga ccccgagaac
     7801 cagcaaacat gtctcccagc ccctgagagc ccctttgcta cccgttcccc caagcaaatc
     7861 aagattgagt cttcgggggc tgtgactgtg ctctcaacca cctgcttcca ttcagaggag
     7921 ggaggacagg aggccacacc caccaaggct gagaacccac tcacacccac cctcagtggc
     7981 ttcttggagt cacctcttaa gtacctggac acacccacca agagtctgct ggacacacct
     8041 gccaagagag cccaggccga gttccccacc tgcgattgcg tcgaacaaat agtggagaaa
     8101 gatgaaggtc catattatac tcacttggga tctggcccca cggtcgcctc tatccgggaa
     8161 ctcatggagg agcggtatgg agagaagggg aaagccatcc ggatcgagaa ggtcatctac
     8221 acggggaagg agggaaagag ctcccgcggt tgccccattg caaagtgggt gatccgcagg
     8281 cacacgctgg aggagaagct actctgcctg cttcggcacc gggcaggcca ccactgccag
     8341 aacgctgtga tcgtcatcct catcctggcc tgggagggca ttccccgtag cctcggagac
     8401 accctctacc aggagctcac cgacaccctc cggaagtatg ggaaccccac cagccggaga
     8461 tgcggcctca acgatgaccg gacctgcgct tgccaaggca aagaccccaa cacctgtggt
     8521 gcctccttct cctttggttg ttcctggagc atgtacttca acggctgcaa gtatgctcgg
     8581 agcaagacac ctcgcaagtt ccgcctcgca ggggacaatc ccaaagagga agaagtgctc
     8641 cggaagagtt tccaggacct ggccaccgaa gtcgctcccc tgtacaagcg actggcccct
     8701 caggcctatc agaaccaggt gaccaacgag gaaatagcga ttgactgccg tctggggctg
     8761 aaggaaggac ggcccttcgc gggggtcacg gcctgcatgg acttctgtgc ccacgcccac
     8821 aaggaccagc ataacctcta caatgggtgc accgtggtct gcaccctgac caaggaagac
     8881 aatcgctgcg tgggcaagat tcccgaggat gagcagctgc atgttctccc cctgtacaag
     8941 atggccaaca cggatgagtt tggtagcgag gagaaccaga atgcaaaggt gggcagcgga
     9001 gccatccagg tgctcaccgc cttcccccgc gaggtccgac gcctgcccga gcctgccaag
     9061 tcctgccgcc agcggcagct ggaagccaga aaggcagcag ccgagaagaa gaagattcag
     9121 aaggagaagc tgagcactcc ggagaagatc aagcaggagg ccctggagct ggcgggcatt
     9181 acgtcggacc caggcctgtc tctgaagggt ggattgtccc agcaaggcct gaagccctcc
     9241 ctcaaggtgg agccgcagaa ccacttcagc tccttcaagt acagcggcaa cgcggtggtg
     9301 gagagctact cggtgctggg caactgccgg ccctccgacc cttacagcat gaacagcgtg
     9361 tactcctacc actcctacta tgcacagccc agcctgacct ccgtcaatgg cttccactcc
     9421 aagtacgctc tcccgtcttt tagctactat ggctttccat ccagcaaccc cgtcttcccc
     9481 tctcagttcc tgggtcctgg tgcctggggg catagtggca gcagtggcag ttttgagaag
     9541 aagccagacc tccacgctct gcacaacagc ctgagcccgg cctacggtgg tgctgagttt
     9601 gccgagctgc ccagccaggc tgttcccaca gacgcccacc accccactcc tcaccaccag
     9661 cagcctgcgt acccaggccc caaggagtat ctgcttccca aggcccccct actccactca
     9721 gtgtccaggg acccctcccc ctttgcccag agctccaact gctacaacag atccatcaag
     9781 caagagccag tagacccgct gacccaggct gagcctgtgc ccagagacgc tggcaagatg
     9841 ggcaagacac ctctgtccga ggtgtctcag aatggaggac ccagtcacct ttggggacag
     9901 tactcaggag gcccaagcat gtcccccaag aggactaacg gtgtgggtgg cagctggggt
     9961 gtgttctcgt ctggggagag tcctgccatc gtccctgaca agctcagttc ctttggggcc
    10021 agctgcctgg ccccttccca cttcacagat ggccagtggg ggctgttccc cggtgagggg
    10081 cagcaggcag cttcccactc tggaggacgg ctgcgaggca aaccgtggag cccctgcaag
    10141 tttgggaaca gcacctcggc cttggctggg cccagcctga ctgagaagcc gtgggcgctg
    10201 ggggcagggg atttcaactc ggccctgaaa ggtagtcctg ggttccaaga caagctgtgg
    10261 aaccccatga aaggagagga gggcaggatt ccagccgcag gggccagcca gctggacagg
    10321 gcctggcagt cctttggtct gcccctggga tccagcgaga agctgtttgg ggctctgaag
    10381 tcagaggaga agctgtggga ccccttcagc ctggaggagg ggccggctga ggagcccccc
    10441 agcaagggag cggtgaagga ggagaagggc ggtggtggtg cggaggagga agaggaggag
    10501 ctgtggtcgg acagtgaaca caacttcctg gacgagaaca tcggcggcgt ggccgtggcc
    10561 ccagcccacg gctccatcct catcgagtgt gcccggcggg agctgcacgc caccacgccg
    10621 cttaagaagc ccaaccgctg ccaccccacc cgcatctcgc tggtcttcta ccagcacaag
    10681 aacctcaacc agcccaacca cgggctggcc ctctgggaag ccaagatgaa gcagctggcg
    10741 gagagggcac gggcacggca ggaggaggct gcccggctgg gcctgggcca gcaggaggcc
    10801 aagctctacg ggaagaagcg caagtggggg ggcactgtgg ttgctgagcc ccagcagaaa
    10861 gagaagaagg gggtcgtccc cacccggcag gcactggctg tgcccacaga ctcggcggtc
    10921 accgtgtcct cctatgccta cacgaaggtc actggcccct acagccgctg gatcctgtga
    10981 attctgcaga tatccagcac agtggcggcc gctcgagtct agagggcccg tttaaacccg
    11041 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
    11101 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
    11161 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
    11221 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
    11281 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
    11341 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
    11401 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
    11461 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
    11521 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
    11581 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
    11641 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
    11701 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
    11761 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
    11821 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
    11881 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
    11941 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
    12001 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
    12061 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
    12121 attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag
    12181 tataatacga caaggtgagg aactaaacca tggccaagtt gaccagtgcc gttccggtgc
    12241 tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgac
//
