LOCUS       sequence-272048-        5480 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV backbone, mEGFP-CREB donor for G-CREB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5480)
  AUTHORS   Laviv T, Scholl B, Parra-Bueno P, Foote B, Zhang C, Yan L, Hayano Y,
            Chu J, Yasuda R
  TITLE     In Vivo Imaging of the Coupling between Neuronal and CREB Activity 
            in the Mouse Brain.
  JOURNAL   Neuron. 2019 Dec 17. pii: S0896-6273(19)31037-2. doi: 
            10.1016/j.neuron.2019.11.028.
  PUBMED    31883788
REFERENCE   2  (bases 1 to 5480)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2019 Dec 17. pii: S0896-6273(19)31037-2. doi: 
            10.1016/j.neuron.2019.11.028."
COMMENT     Sequence Label: AAV-SYN_mEGFP-CREB
FEATURES             Location/Qualifiers
     source          1..5480
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        210..657
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      682..691
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(688..690,691..693,694..1404)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(733..754)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(994..1013)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1341..1362
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     repeat_region   2743..2883
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2743..2872
                     /label=AAV2 ITR
     rep_origin      2958..3413
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3045..3064)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3255..3276
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3430..3449)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3549..3571
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3609..3627)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3695..3799
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3800..3868,3869..4660)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4018..4037)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4831..5419
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5320..5339
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtattacc gccatgcatt agttattaat agtaatcaat
      181 tacggggtct gcagagggcc ctgcgtatga gtgcaagtgg gttttaggac caggatgagg
      241 cggggtgggg gtgcctacct gacgaccgac cccgacccac tggacaagca cccaaccccc
      301 attccccaaa ttgcgcatcc cctatcagag agggggaggg gaaacaggat gcggcgaggc
      361 gcgtgcgcac tgccagcttc agcaccgcgg acagtgcctt cgcccccgcc tggcggcgcg
      421 cgccaccgcc gcctcagcac tgaaggcgcg ctgacgtcac tcgccggtcc cccgcaaact
      481 ccccttcccg gccaccttgg tcgcgtccgc gccgccgccg gcccagccgg accgcaccac
      541 gcgaggcgcg agataggggg gcacgggcgc gaccatctgc gctgcggcgc cggcgactca
      601 gcgctgcctc agtctgcggt gggcagcgga ggagtcgtgt cgtgcctgag agcgcagtcg
      661 aattggctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
      721 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      781 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      841 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
      901 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      961 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     1021 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     1081 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     1141 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     1201 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     1261 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac
     1321 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     1381 ctcggcatgg acgagctgta caagcctacc cctaccccta ccatgaccat ggactctgga
     1441 gcagacaacc agcagagtgg agatgctgct gtaacagaag ctgaaagtca acaaatgaca
     1501 gttcaagccc agccacagat tgccacatta gcccaggtat ccatgccagc agctcatgcg
     1561 acgtcatctg ctcccactgt aaccttagtg cagctgccca atgggcagac agtccaggtc
     1621 catggggtca tccaggcggc ccagccatca gttattcagt ctccacaagt ccaaacagtt
     1681 cagtcttcct gtaaggactt aaaaagactt ttctccggaa ctcagatttc aactattgca
     1741 gaaagtgaag attcacagga gtctgtggat agtgtaactg attcccaaaa acgaagggaa
     1801 atcctttcaa ggaggccttc ctacaggaaa attttgaatg acttatcttc tgatgcacca
     1861 ggggtgccaa ggattgaaga agaaaaatca gaagaagaga cttcagcccc tgccatcacc
     1921 actgtaacag tgccaacccc gatttaccaa actagcagtg ggcagtatat tgccattacc
     1981 cagggaggag caatacagct ggctaacaat ggtaccgatg gggtacaggg cctgcagaca
     2041 ttaaccatga ccaatgcagc tgccactcag ccgggtacta ccattctaca atatgcacag
     2101 accactgatg gacagcagat tctagtgccc agcaaccaag ttgttgttca agctgcctct
     2161 ggtgatgtac aaacatacca gattcgcaca gcacccacta gcaccattgc ccctggagtt
     2221 gttatggcgt cctccccagc acttcctaca cagcctgctg aagaagcagc acgaaagaga
     2281 gaggttcgtc taatgaagaa cagggaagca gcaagagaat gtcgtagaaa gaagaaagaa
     2341 tatgtgaaat gtttagagaa cagagtggca gtgcttgaaa accaaaacaa aacattgatt
     2401 gaggagctaa aagcacttaa ggacctttac tgccacaagt cagattgatc cggactcaga
     2461 tctcgagctc aagcttgtgg catccctgtg acccctcccc agtgcctctc ctggccctgg
     2521 aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc atcattttgt
     2581 ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg agcaaggggc
     2641 aagttgggaa gacccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc
     2701 cttctgattt tgtaggtaac cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg
     2761 agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg
     2821 cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc
     2881 aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac
     2941 gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     3001 tacgcgcagc gtgaccgcta cacttgccag cgccttagcg cccgctcctt tcgctttctt
     3061 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     3121 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga
     3181 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     3241 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaact ctatctcggg
     3301 ctattctttt gatttataag ggattttgcc gatttcggtc tattggttaa aaaatgagct
     3361 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg
     3421 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     3481 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     3541 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     3601 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     3661 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     3721 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     3781 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     3841 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     3901 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     3961 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     4021 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     4081 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     4141 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     4201 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     4261 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     4321 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     4381 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     4441 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     4501 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     4561 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     4621 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     4681 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     4741 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     4801 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     4861 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     4921 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     4981 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5041 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     5101 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     5161 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     5221 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     5281 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     5341 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     5401 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     5461 ctggcctttt gctcacatgt
//
