LOCUS       sequence-272049-        5421 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV backbone, mCherry-KIX acceptor for G-CREB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5421)
  AUTHORS   Laviv T, Scholl B, Parra-Bueno P, Foote B, Zhang C, Yan L, Hayano Y,
            Chu J, Yasuda R
  TITLE     In Vivo Imaging of the Coupling between Neuronal and CREB Activity 
            in the Mouse Brain.
  JOURNAL   Neuron. 2019 Dec 17. pii: S0896-6273(19)31037-2. doi: 
            10.1016/j.neuron.2019.11.028.
  PUBMED    31883788
REFERENCE   2  (bases 1 to 5421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2019 Dec 17. pii: S0896-6273(19)31037-2. doi: 
            10.1016/j.neuron.2019.11.028."
COMMENT     Sequence Label: AAV-SYN_mCherry-KIX-mCherry
FEATURES             Location/Qualifiers
     source          1..5421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        210..657
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      682..691
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             688..1395
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             1681..2391
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     repeat_region   2684..2824
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2684..2813
                     /label=AAV2 ITR
     rep_origin      2899..3354
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2986..3005)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3196..3217
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3371..3390)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3490..3512
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3550..3568)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3636..3740
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3741..3809,3810..4601)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3959..3978)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4772..5360
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5261..5280
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtattacc gccatgcatt agttattaat agtaatcaat
      181 tacggggtct gcagagggcc ctgcgtatga gtgcaagtgg gttttaggac caggatgagg
      241 cggggtgggg gtgcctacct gacgaccgac cccgacccac tggacaagca cccaaccccc
      301 attccccaaa ttgcgcatcc cctatcagag agggggaggg gaaacaggat gcggcgaggc
      361 gcgtgcgcac tgccagcttc agcaccgcgg acagtgcctt cgcccccgcc tggcggcgcg
      421 cgccaccgcc gcctcagcac tgaaggcgcg ctgacgtcac tcgccggtcc cccgcaaact
      481 ccccttcccg gccaccttgg tcgcgtccgc gccgccgccg gcccagccgg accgcaccac
      541 gcgaggcgcg agataggggg gcacgggcgc gaccatctgc gctgcggcgc cggcgactca
      601 gcgctgcctc agtctgcggt gggcagcgga ggagtcgtgt cgtgcctgag agcgcagtcg
      661 aattggctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagga caacatggcc
      721 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
      781 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
      841 aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
      901 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
      961 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
     1021 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     1081 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     1141 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     1201 ctgaaggacg gcggccacta cgacgccgag gtcaagacca cctacaaggc caagaagccc
     1261 gtgcagctgc ccggcgccta caacgtcaac atcaagctgg acatcacctc ccacaacgag
     1321 gactacacca tcgtggaaca gtacgagcgc gccgagggcc gccactccac cggcggcatg
     1381 gacgagctgt acaagtccgg actcagatct cgagctcaag cttcgaattg gcatgaacat
     1441 gtgactcagg acctacggag tcatctagtc cataaactcg ttcaagccat cttcccaact
     1501 ccagaccctg cagctctgaa agatcgccgc atggagaacc tggttgccta tgctaagaaa
     1561 gtggagggag acatgtatga gtctgctaat agcagggatg aatactatca tttattagca
     1621 gagaaaatct ataaaataca aaaatctgca gtcgacggta ccgcgggccc gggatccgga
     1681 atggtgagca agggcgagga ggacaacatg gccatcatca aggagttcat gcgcttcaag
     1741 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
     1801 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc
     1861 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
     1921 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
     1981 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
     2041 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
     2101 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
     2161 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgcc
     2221 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
     2281 aacatcaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag
     2341 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta agcggccgcg
     2401 actctagatc aaagcttgtg gcatccctgt gacccctccc cagtgcctct cctggccctg
     2461 gaagttgcca ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg
     2521 tctgactagg tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg
     2581 caagttggga agacccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt
     2641 ccttctgatt ttgtaggtaa ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg
     2701 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc
     2761 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg
     2821 caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     2881 cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     2941 ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct
     3001 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     3061 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg
     3121 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     3181 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg
     3241 gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc
     3301 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt
     3361 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     3421 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     3481 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     3541 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     3601 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     3661 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     3721 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     3781 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     3841 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     3901 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     3961 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     4021 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     4081 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     4141 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     4201 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     4261 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     4321 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     4381 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     4441 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     4501 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     4561 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     4621 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     4681 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     4741 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     4801 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     4861 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     4921 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     4981 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     5041 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     5101 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     5161 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     5221 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     5281 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     5341 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     5401 gctggccttt tgctcacatg t
//
