LOCUS       sequence-272057-       10308 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMV promoter expression plasmid for 
            TadA*(V82G)-nCas9-pmCDA1(R187W)-UGI-UGI-P2A-EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10308)
  AUTHORS   Grunewald J, Zhou R, Lareau CA, Garcia SP, Iyer S, Miller BR, 
            Langner LM, Hsu JY, Aryee MJ, Joung JK
  TITLE     A dual-deaminase CRISPR base editor enables concurrent adenine and 
            cytosine editing.
  JOURNAL   Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0535-y. doi: 
            10.1038/s41587-020-0535-y.
  PUBMED    32483364
REFERENCE   2  (bases 1 to 10308)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0535-y. doi: 
            10.1038/s41587-020-0535-y."
COMMENT     Sequence Label: SPACE (pRZ1813)
FEATURES             Location/Qualifiers
     source          1..10308
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    607..628
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(643..648,649..666,667..673)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    681..697
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     686..708
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     705..721
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     705..721
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    792..1016
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     1005..1022
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(1045..1062)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(1071..1091)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(1104..1814,1815..1817,1818..1820))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(1104..1814,1815..1817,1818..1820))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1146..1167)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1495..1514
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             complement(1821..1877)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(1887..2135)
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             complement(2166..2414)
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             complement(3162..3182)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(3225..7298,7299..7301,7302..7325))
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(3225..7325)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(7920..7940)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(7989..8008)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7990..8008)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(8050..8253)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8079..8099)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8254..8633
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     8805..8824
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8903..9007
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9008..9076,9077..9868)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9226..9245)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(10039..10308,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
      601 cgcaattaat gtgagttagc tcactcatta ggcaccctag gctttacact ttatgcttcc
      661 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
      721 ccatgattac gccaagctct agctagaggt cgacggtatc gagccccagc tggttctttc
      781 cgcctcagaa gccatagagc ccaccgcatc cccagcatgc ctgctattgt cttcccaatc
      841 ctcccccttg ctgtcctgcc ccaccccacc ccccagaata gaatgacacc tactcagaca
      901 atgcgatgca atttcctcat tttattagga aaggacagtg ggagtggcac cttccagggt
      961 caaggaaggc acgggggagg ggcaaacaac agatggctgg caactagaag gcacagtcga
     1021 ggctgatcag cgggtttaaa ctcaatggtg atggtgatga tgaccggtta gactttcctc
     1081 ttcttcttgg gagaaccacc agacttgtac agctcgtcca tgccgagagt gatcccggcg
     1141 gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg
     1201 gcggactggg tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg
     1261 gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc
     1321 ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg
     1381 ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc
     1441 ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg
     1501 tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg
     1561 gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca ctgcactcca
     1621 taggtcaggg tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg
     1681 aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac
     1741 ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc
     1801 tcctcgccct tgctcaccat aggtccaggg ttctcctcca cgtctccagc ctgcttcagc
     1861 aggctgaagt tagtagctcc gcttcccagc attttgattt tgttctctcc gttggaatcc
     1921 tggatgacca gagcccaggg cttatactct ggggcgtcgg atgtcagcag catcacattt
     1981 tcgtcggtac tctcgtcgta ggcggtatgg accaggatat cgctctcagg cttgtttccg
     2041 atcacttctt cgacttcttc gggcagcatc aggatgctct cctggatgac cagctgcttg
     2101 cctgtctcct tctcgatgat gtcagacagg ttggtgctgc ctccagatcc tccggatcct
     2161 ccgctcagca tcttgatctt attctcgccg ttagaatcct ggatgaccag ggcccaaggc
     2221 ttatactcgg gggcgtcaga ggtcagcagc atcacattct catctgtgga ctcgtcgtag
     2281 gcggtgtgca ccaggatgtc agactctggc ttgttgccga tcacttcctc cacctcctca
     2341 ggcagcatca ggatggactc ctgaatgacc agctgtttcc cagtctcctt ctcaatgatg
     2401 tcgctcagat tagtgctccc cccgctcccg ccgctcccgc cgctaacggc tggagactta
     2461 gtggtatgca gaatcttgac ctggatcatg atgctcagct cggatctcca cttctccgca
     2521 cgtttcagtg ttttctccaa ccagcggttt tcattgagct ggttatgaga gctttggatg
     2581 aaaatcttcc gacaacactg atagtgctcg gaaaccatca cgttcaggcc tacaccattg
     2641 tccctcagat tccagagtcc tatctggttc cgggcattct tctcgtagta cagcttgcag
     2701 gcccagattt tcaatgtgtg cccgtttccc cgaagttcct gattatacca ttccagaatt
     2761 ttctcggcac aatcagcgca aggactccag ctggaatacc agttgatcgt aaactgccca
     2821 ggattatccc gaagatactc ttccaccttt cggatactga atatctcagc gtgtattcct
     2881 ctctcggtgc cactttgggg cttgttgacg gcatagcccc aaaagcaagc ccttctttcg
     2941 ccccttcgct tcaactcaaa cagcacgtag cagcggtgtg acacagattt cttgttgttg
     3001 aagaactgct tcttgaaggt gtaaatatcc agtttctcat gaatgcgcac gtactcggcg
     3061 tctgtggatc ctccggagga gccgccggag ctctctggtg tggcggactc gcttgtgcct
     3121 ggtgtctcgc ttccagagga tcctccgcta gatcctccag agactttcct cttcttcttg
     3181 ggctcgaatt cgctgccgtc ggcggttctt tttgagccgc cagagtcacc tcccagctga
     3241 gacaggtcga tccgtgtctc gtacaggccg gtgatgctct ggtggatcag ggtggcgtcc
     3301 agcacctctt tggtgctggt gtacctcttc cggtcgatgg tggtgtcaaa gtacttgaag
     3361 gcggcagggg ctcccagatt ggtcagggta aacaggtgga tgatattctc ggcctgctct
     3421 ctgatgggct tatcccggtg cttgttgtag gcggacagca ctttgtccag attagcgtcg
     3481 gccaggatca ctctcttgga gaactcgctg atctgctcga tgatctcgtc caggtagtgc
     3541 ttgtgctgtt ccacaaacag ctgtttctgc tcattatcct cgggggagcc cttcagcttc
     3601 tcatagtggc tggccaggta caggaagttc acatatttgg agggcagggc cagttcgttt
     3661 cccttctgca gttcgccggc agaggccagc attctcttcc ggccgttttc cagctcgaac
     3721 agggagtact taggcagctt gatgatcagg tcctttttca cttctttgta gcccttggct
     3781 tccagaaagt cgatgggatt cttctcgaag ctgcttcttt ccatgatggt gatccccagc
     3841 agctctttca cactcttcag tttcttggac ttgccctttt ccactttggc caccaccagc
     3901 acagaatagg ccacggtggg gctgtcgaag ccgccgtact tcttagggtc ccagtccttc
     3961 tttctggcga tcagcttatc gctgttcctc ttgggcagga tagactcttt gctgaagccg
     4021 cctgtctgca cctcggtctt tttcacgata ttcacttggg gcatgctcag cactttccgc
     4081 acggtggcaa aatcccggcc cttatcccac acgatctccc cggtttcgcc gtttgtctcg
     4141 atcagaggcc gcttccggat ctcgccgttg gccagggtaa tctcggtctt gaaaaagttc
     4201 atgatgttgc tgtagaagaa gtacttggcg gtagccttgc cgatttcctg ctcgctcttg
     4261 gcgatcatct tccgcacgtc gtacaccttg tagtcgccgt acacgaactc gctttccagc
     4321 ttagggtact ttttgatcag ggcggttccc acgacggcgt tcaggtaggc gtcgtgggcg
     4381 tggtggtagt tgttgatctc gcgcactttg taaaactgga aatccttccg gaaatcggac
     4441 accagcttgg acttcagggt gatcactttc acttcccgga tcagcttgtc attctcgtcg
     4501 tacttagtgt tcatccggga gtccaggatc tgtgccacgt gctttgtgat ctgccgggtt
     4561 tccaccagct gtctcttgat gaagccggcc ttatccagtt cgctcaggcc gcctctctcg
     4621 gccttggtca gattgtcgaa ctttctctgg gtaatcagct tggcgttcag cagctgccgc
     4681 cagtagttct tcatcttctt cacgacctct tcggagggca cgttgtcgct cttgccccgg
     4741 ttcttgtcgc ttctggtcag caccttgttg tcgatggagt cgtccttcag aaagctctga
     4801 ggcacgatat ggtccacatc gtagtcggac agccggttga tgtccagttc ctggtccacg
     4861 tacatatccc gcccattctg caggtagtac aggtacagct tctcgttctg cagctgggtg
     4921 ttttccacgg ggtgttcttt caggatctgg ctgcccagct ctttgatgcc ctcttcgatc
     4981 cgcttcattc tctcgcggct gttcttctgt cccttctggg tggtctggtt ctctctggcc
     5041 atttcgatca cgatgttctc gggcttgtgc cggcccatca ctttcacgag ctcgtccacc
     5101 accttcactg tctgcaggat gcccttctta atggcggggc tgccggccag attggcaatg
     5161 tgctcgtgca ggctatcgcc ctggccggac acctgggctt tctggatgtc ctctttaaag
     5221 gtcaggctgt cgtcgtggat cagctgcatg aagtttctgt tggcgaagcc gtcggacttc
     5281 aggaaatcca ggattgtctt gccggactgc ttgtcccgga tgccgttgat cagcttccgg
     5341 ctcagcctgc cccagccggt gtatctccgc cgcttcagct gcttcatcac tttgtcgtcg
     5401 aacaggtggg cataggtttt cagccgttcc tcgatcatct ctctgtcctc aaacagtgtc
     5461 agggtcagca cgatatcttc cagaatgtcc tcgttttcct cattgtccag gaagtccttg
     5521 tccttgataa ttttcagcag atcgtggtat gtgcccaggg aggcgttgaa ccgatcttcc
     5581 acgccggaga tttccacgga gtcgaagcac tcgattttct tgaagtagtc ctctttcagc
     5641 tgcttcacgg tcactttccg gttggtcttg aacagcaggt ccacgatggc ctttttctgc
     5701 tcgccgctca ggaaggcggg ctttctcatt ccctcggtca cgtatttcac tttggtcagc
     5761 tcgttataca cggtgaagta ctcgtacagc aggctgtgct tgggcagcac cttctcgttg
     5821 ggcaggttct tatcgaagtt ggtcatccgc tcgatgaagc tctgggcgga agcgcccttg
     5881 tccaccactt cctcgaagtt ccagggggtg atggtttcct cgctctttct ggtcatccag
     5941 gcgaatctgc tgtttcccct ggccagaggg cccacgtagt aggggatgcg gaaggtcagg
     6001 atcttctcga tcttttcccg gttgtccttc aggaatgggt aaaaatcttc ctgccgccgc
     6061 agaatggcgt gcagctctcc caggtggatc tggtggggga tgctgccgtt gtcgaaggtc
     6121 cgctgcttcc gcagcaggtc ctctctgttc agcttcacga gcagttcctc ggtgccgtcc
     6181 atcttttcca ggatgggctt gatgaacttg tagaactctt cctggctggc tccgccgtca
     6241 atgtagccgg cgtagccgtt cttgctctgg tcgaagaaaa tctctttgta cttctcaggc
     6301 agctgctgcc gcacgagagc tttcagcagg gtcaggtcct ggtggtgctc gtcgtatctc
     6361 ttgatcatag aggcgctcag gggggccttg gtgatctcgg tgttcactct caggatgtcg
     6421 ctcagcagga tggcgtcgga caggttcttg gcggccagaa acaggtcggc gtactggtcg
     6481 ccgatctggg ccagcaggtt gtccaggtcg tcgtcgtagg tgtccttgct cagctgcagt
     6541 ttggcatcct cggccaggtc gaagttgctc ttgaagttgg gggtcaggcc caggctcagg
     6601 gcaatcaggt ttccgaacag gccattcttc ttctcgccgg gcagctgggc gatcagattt
     6661 tccagccgtc tgctcttgct cagtctggca gacaggatgg ccttggcgtc cacgccgctg
     6721 gcgttgatgg ggttttcctc gaacagctgg ttgtaggtct gcaccagctg gatgaacagc
     6781 ttgtccacgt cgctgttgtc ggggttcagg tcgccctcga tcaggaagtg gccccggaac
     6841 ttgatcatgt gggccagggc cagatagatc agccgcaggt cggccttgtc ggtgctgtcc
     6901 accagtttct ttctcaggtg gtagatggtg gggtacttct cgtggtaggc cacctcgtcc
     6961 acgatgttgc cgaagatggg gtgccgctcg tgcttcttat cctcttccac caggaaggac
     7021 tcttccagtc tgtggaagaa gctgtcgtcc accttggcca tctcgttgct gaagatctct
     7081 tgcagatagc agatccggtt cttccgtctg gtgtatcttc ttctggcggt tctcttcagc
     7141 cgggtggcct cggctgtttc gccgctgtcg aacagcaggg ctccgatcag gttcttcttg
     7201 atgctgtgcc ggtcggtgtt gcccagcacc ttgaatttct tgctgggcac cttgtactcg
     7261 tcggtgatca cggcccagcc cacagagttg gtgccgatgg ccaggccgat gctgtacttc
     7321 ttgtctgacc ccccgctgct gcccccgctg ctttcaggtg ttgcgctctc gcttgtgcca
     7381 ggtgtctcag agccagagga gcctccgcta gatcctccgg agtcggtgga gctctgggcc
     7441 ttcttctgag cattgaacac ctgtctaggc atccgaaaga aatagcacag cagggcggca
     7501 cattcatctg ccaggattcc ctcggtaatt tcgacgcggt gattcatgcc ggggtagtgc
     7561 agcacgtcca tcagggagcc tgcggcgccg gtttttgcgt tcctcacgcc aaacaccacg
     7621 cggccgatcc tagagtggat catggcgccg gcgcacatca cgcaaggctc gaatgtgccg
     7681 tacagggtgg cgtcaatcag tctgtagttc tgcatgacca ggccgccctg tctcagggcc
     7741 ataatttcgg catgggctgt tgggtcgtgc aggccgatgg ctctgttcca gccctcgccg
     7801 atcactctat tgttcagcac cagcacggct cccacaggca cctccctctc atcgcgtgcc
     7861 ctcttggcca gggtcagggc atgtctcatc cagtactcgt gggaaaactc cacctcagag
     7921 actttccgct tcttctttgg tgactcgaac tcgcttccgt cggctgtccg tttcatggtg
     7981 gcggctctcc ctatagtgag tcgtattagc ggccgcggat ctctagcgga tctgacggtt
     8041 cactaaacca gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt
     8101 caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa
     8161 actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc
     8221 acgcccattg atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta
     8281 gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg
     8341 ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac
     8401 tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat
     8461 tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg
     8521 tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg
     8581 aactaatgac cccgtaattg attactatta ataactagtc aataatcaat gtcaacgcgt
     8641 atatctggcc cgtacatcgc gaagcagcgc aaaacgccta accctaagca gattcttcat
     8701 gcaattgtcg gtcaagcctt gccttgttgt agcttaaatt ttgctcgcgc actactcagc
     8761 gacctccaac acacaagcag ggagcagata ctggcttaac tatgcggcat cagagcagat
     8821 tgtactgaga gtcgacccta ggggatcggg agatcgatct cccgatccgt cgacgtcagg
     8881 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     8941 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     9001 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     9061 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     9121 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     9181 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     9241 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     9301 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     9361 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     9421 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     9481 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     9541 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     9601 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     9661 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     9721 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     9781 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     9841 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     9901 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     9961 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagtgtcag accccgtaga
    10021 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
    10081 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
    10141 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
    10201 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
    10261 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgg
//
