LOCUS       sequence-272066-       11843 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for expressing NEK10 under the control of the 
            endogenous NEK10 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11843)
  AUTHORS   Chivukula RR, Montoro DT, Leung HM, Yang J, Shamseldin HE, Taylor 
            MS, Dougherty GW, Zariwala MA, Carson J, Daniels MLA, Sears PR, 
            Black KE, Hariri LP, Almogarri I, Frenkel EM, Vinarsky V, Omran H, 
            Knowles MR, Tearney GJ, Alkuraya FS, Sabatini DM
  TITLE     A human ciliopathy reveals essential functions for NEK10 in airway 
            mucociliary clearance.
  JOURNAL   Nat Med. 2020 Jan 20. pii: 10.1038/s41591-019-0730-x. doi: 
            10.1038/s41591-019-0730-x.
  PUBMED    31959991
REFERENCE   2  (bases 1 to 11843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Med. 
            2020 Jan 20. pii: 10.1038/s41591-019-0730-x. doi: 
            10.1038/s41591-019-0730-x."
COMMENT     Sequence Label: pLRC1-NEK10p:NEK10-3XFLAG
FEATURES             Location/Qualifiers
     source          1..11843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        182..408
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             409..589
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    636..761
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1254..1487
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1672..1716
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             7005..7070
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    7118..7235
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7284..7794
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7713..7731
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7816..8415
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7816..7835)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8312..8332
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8543..8776
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8848..8969
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8885..8904)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8939..8958
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9009..9144
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9164..9183)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9165..9183)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9193..9210)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9193..9209)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9202..9224)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9351..9806
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9438..9457)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9648..9669
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9832..9936
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9937..10005,10006..10797)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10155..10174)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10968..11556
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11457..11476
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11710..11727
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1081 agaccaccgc acagcaagca gcagctgatc ttcagacctg gaggaggaga tatgagggac
     1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1861 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     1921 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgatgc
     1981 ccggcacaaa attaagcact taatagattg gcatttttca tttatttaaa gttacagaat
     2041 gtcaggtata tttttaaagc gttatctaat gcagttcaga aattttaaag aagctccaga
     2101 ctttcacaaa aggtgaagtg acttgcccaa agccacaaaa cgaactggcg atagttatac
     2161 tatacaggtt tcttgaatcc caggtcactc tttttgtgaa aaatacggtg ctgtggcacc
     2221 ttattctcat tgcctgtggc ttgagtaact gaagacgttt tcatgccata tgaaaggtat
     2281 ttagcatgct gcattgattc cttatcagat taaatctttg cttttaaaga gaattgattt
     2341 ttagcccata gcaattcttg aaagtaaaca ctccaagatg aagctgccct ttcccatcaa
     2401 aggttagaca ccctaaatcc agaaaaatct tcaacgtgta ggagacctgt tcacaggtag
     2461 ctctaggttc cggtgaagtg gctgcagcac agccttcagc atcacttgct gcctcttctg
     2521 cttgcaagct tggtggtgga tctagcgcgt ctctggttgc tacggcgatt ccccggcagg
     2581 cgtcatccgt cgctatggcg ttctgcactg cgcagcatgg ctgctcccga ttggctgagg
     2641 cgccccaggt ggcggaggaa gaattgtcgg cattggcctg gccagcgctg gttggtggcc
     2701 tggaggagga tttgattgga aaaccaacgg tgcagctggc cgcggtgtcc ctgaggttga
     2761 ggggaccggg aataggctgg ggggaggacg ggacgggctg agactggacg ggacccccgg
     2821 tctgcagcag caggtgacag cagcagggac aatgataagg agattggcct gaaggaggga
     2881 ccgtccctcc cgcgcggtga gtaggcggga gccggtgctc ggctgtgccg gcgagcagct
     2941 tgagtcaggg tctccgggga gacgtcctca ggtccctgtg gagcggcccc gaagcctcgg
     3001 gagccagctt cactagacaa gaggcagagg tagagaatgc ggctgtggtg ggtaagtgag
     3061 tcactgcgtg aggcagtctt ttcaaagcag gtgggtttgt gttggacgga tggatggtgg
     3121 gaaagcgcca tcaacgatgc ttcttggcgc ttccattttg ataacggaat tattctggtt
     3181 aaatggagcc tgaatgtgtc tcttccaatc tgctccgacg cctctggagg ttcattcttc
     3241 atctctatga gcgtcgcttt tctgccttct ccttacagga tgctgctttg actttgcccc
     3301 tctgcttttc tgccctactt cggtgagccc ttccacttcc tcgcctcagg caccatctat
     3361 ctgacgatga ttagaaagtc tccaactgga acctagcctg ctctactaac tgtcagaccc
     3421 caaaatccag ctgccaagtt aacgccacac atgtgcaacc ctgaacgcca tcttccccat
     3481 gtctgctgct gctcttggat tccctatttt gggcatcact atccattcag ctttcaaaaa
     3541 ctacactcta gacgacatcc atgacactta ctgctgcttc ctctgcatca ccggtttaat
     3601 taaccaccat gcctgatcaa gataaaaagg tgaagaccac agaaaaatca actgataaac
     3661 agcaagaaat caccatcagg gactattcag atcttaaaag acttcggtgc cttttgaacg
     3721 tccaatcaag caaacaacag cttccagcca ttaacttcga tagtgcccaa aatagcatga
     3781 cgaagtctga gcccgccatc agggcgggtg gacacagagc tcggggtcag tggcatgaat
     3841 ccacagaagc tgttgaactt gaaaatttta gtataaacta caagaatgag agaaatttca
     3901 gcaaacatcc tcagcgtaaa ctatttcagg agatctttac cgccttggtg aaaaatagac
     3961 tcataagcag agagtgggtt aatcgagccc catctattca ttttctgaga gtgttaatct
     4021 gtctgaggct actaatgagg gatccatgtt atcaggaaat actccatagc ttgggtggga
     4081 ttgaaaacct agctcagtat atggagattg tagccaatga gtacctcggc tatggagaag
     4141 agcagcacac tgtggacaag ctggtcaaca tgacatatat ttttcaaaaa cttgctgcag
     4201 tcaaagatca aagagaatgg gtcaccacaa gtggagccca caagacatta gtaaatttac
     4261 ttggtgcccg agatactaat gttctattgg gttcccttct ggctctggct agtttagcag
     4321 aaagtcaaga atgtagggag aagataagtg aactcaacat tgtagaaaat ctgttgatga
     4381 ttttacatga atatgacttg ctttctaaaa gactaacagc ggagttgctg cgcctacttt
     4441 gtgcagagcc ccaggtgaaa gagcaggtga agctctatga ggggataccg gtcctcctca
     4501 gtctgctcca ctctgaccac ttgaagctcc tctggagcat tgtctggatt ctggtacagg
     4561 tttgtgagga ccctgagacc agcgtggaaa ttcgcatttg gggaggcatc aaacagcttc
     4621 ttcatatttt acaaggagac agaaattttg tttctgatca ctcctccatt ggaagcctgt
     4681 ccagtgcaaa tgctgcaggc cgaatccagc agcttcattt atcagaagac ttgagcccta
     4741 gggaaataca agaaaatact ttctcacttc aagcagcctg ctgtgctgcc ctcactgagc
     4801 tggtgctcaa tgacaccaat gcccaccagg tggttcagga aaatggtgta tatacaatag
     4861 caaaattaat tttaccaaat aagcaaaaga atgcagcaaa aagtaatcta ttacagtgtt
     4921 atgctttcag agccttgaga tttctcttca gtatggaaag aaacagacca ctctttaaaa
     4981 gacttttccc cacagacttg tttgagatct tcattgacat agggcattat gtacgtgata
     5041 tcagtgctta tgaagaattg gtatccaagc tgaatttatt agtggaggat gaactgaagc
     5101 aaattgctga aaatattgaa agcattaatc agaacaaagc tcctttgaaa tatataggca
     5161 actatgcaat tttggatcat cttggaagtg gagcttttgg ctgtgtttac aaggttagaa
     5221 agcatagtgg tcaaaatctt ttagcaatga aagaggtcaa tttacataac ccagcatttg
     5281 gaaaggataa gaaagatcga gacagcagcg taaggaatat tgtttctgaa ttaacaataa
     5341 ttaaagagca gctttatcat cccaacattg tacgttatta caaaacattt ctggaaaatg
     5401 ataggttgta catagttatg gagctgatag aaggagcccc gcttggagag catttcagtt
     5461 ctttgaagga aaaacatcac cattttactg aagaaagact atggaaaata tttatacagc
     5521 tgtgcttagc tcttcgatac ttacacaagg agaagaggat tgtccataga gatctgacac
     5581 caaacaacat tatgttgggg gataaggaca aagtaaccgt tactgacttt ggcctggcaa
     5641 agcaaaaaca agaaaacagt aaactcacct ctgtggttgg aacaatcctg tattcttgcc
     5701 ccgaggtact gaagagtgag ccgtatgggg agaaggctga tgtctgggca gtaggctgca
     5761 tcctttatca gatggcgact ttgagtcccc ccttctacag cactaacatg ctgtccttgg
     5821 ctacaaaaat agtggaggcg gtatatgaac cagtcccaga aggtatctac tctgaaaaag
     5881 taacagacac catcagcagg tgcctcactc ctgatgcgga agctcgtcca gatattgtag
     5941 aagtcagttc gatgatatca gatgtcatga tgaaatattt agacaactta tctacatccc
     6001 agttgtcctt ggaaaagaag ctagaacggg aacgaagacg cacacaaagg tattttatgg
     6061 aagccaaccg gaacaccgtc acatgtcacc atgagctggc tgttctatct cacgagacct
     6121 ttgagaaggc aagtttgagt agcagcagca gtggagcagc cagcctgaaa agtgaacttt
     6181 cagaaagcgc agacctgccc cctgaaggct tccaggcctc ctatggtaaa gacgaagaca
     6241 gggcctgtga cgaaatcctg tcagatgata acttcaacct ggaaaatgct gagaaagata
     6301 catattcaga ggtagatgat gaattggaca tttcggataa ctccagcagc tccagttcaa
     6361 gccctctgaa agaatctaca ttcaacattt taaagagaag ttttagtgct tcaggaggag
     6421 aaagacaatc ccaaacaagg gacttcactg gaggaacagg atcaagacca agaccagggc
     6481 cacagatggg cacattcttg tggcaagcat cagcaggaat tgctgtgtcc cagaggaaag
     6541 tgcgtcagat cagtgatcct attcagcaga tattaattca gctgcacaaa ataatctata
     6601 tcacacagct tcctccagct ttgcaccaca atttgaaaag aagggttata gagagattca
     6661 agaaatccct cttcagccag cagagtaacc cttgtaattt gaaatctgaa attaaaaagt
     6721 tatctcaggg atctccagaa ccgattgagc ccaacttttt cacagcagat taccatttat
     6781 tacatcgttc atccggtgga aacagcctgt ccccaaatga ccctacaggt ttaccaacca
     6841 gcattgaatt ggaggaagga ataacatatg aacagatgca gactgtgatt gaagaagtcc
     6901 ttgaggaaag tggctattac aattttacat ctaacaggta tcattcctat ccatggggga
     6961 ccaagaatca cccaaccaaa agaggaggaa gtgcggccgc aggcgactac aaagaccatg
     7021 acggtgatta taaagatcat gacattgatt acaaggatga cgatgacaag taataagaat
     7081 tctcgacctc gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     7141 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     7201 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     7261 atccactttg gccgcggctc gagggggttg gggttgcgcc ttttccaagg cagccctggg
     7321 tttgcgcagg gacgcggctg ctctgggcgt ggttccggga aacgcagcgg cgccgaccct
     7381 gggtctcgca cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt
     7441 gtgggccccc cggcgacgct tcctgctccg cccctaagtc gggaaggttc cttgcggttc
     7501 gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca ctagtaccct cgcagacgga
     7561 cagcgccagg gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc
     7621 tcagcagggc gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg
     7681 gcggtagtgt gggccctgtt cctgcccgcg cggtgttccg cattctgcaa gcctccggag
     7741 cgcacgtcgg cagtcggctc cctcgttgac cgaatcaccg acctctctcc ccagggggat
     7801 ccaccggagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     7861 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     7921 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     7981 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     8041 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     8101 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     8161 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     8221 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     8281 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     8341 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     8401 aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga
     8461 ccccatgcat cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     8521 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     8581 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     8641 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     8701 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     8761 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     8821 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     8881 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     8941 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     9001 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     9061 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     9121 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
     9181 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     9241 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     9301 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     9361 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     9421 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     9481 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     9541 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     9601 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     9661 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     9721 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     9781 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     9841 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     9901 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     9961 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
    10021 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
    10081 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
    10141 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
    10201 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
    10261 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
    10321 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
    10381 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
    10441 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
    10501 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
    10561 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
    10621 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
    10681 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
    10741 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
    10801 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
    10861 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
    10921 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
    10981 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
    11041 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
    11101 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
    11161 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
    11221 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
    11281 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
    11341 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
    11401 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
    11461 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
    11521 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
    11581 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
    11641 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
    11701 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
    11761 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
    11821 gcgggcagtg agcgcaacgc aat
//
