LOCUS       sequence-272084-        9382 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses gRNA using fusion of R. toruloides 5S rRNA and tRNA-Arg as
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9382)
  AUTHORS   Schultz JC, Cao M, Zhao H
  TITLE     Development of a CRISPR/Cas9 system for high efficiency multiplexed 
            gene deletion in Rhodosporidium toruloides.
  JOURNAL   Biotechnol Bioeng. 2019 Apr 30. doi: 10.1002/bit.27001.
  PUBMED    31038202
REFERENCE   2  (bases 1 to 9382)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2019 Apr 30. doi: 10.1002/bit.27001."
COMMENT     Sequence Label: NM1-5S-tRNA-SgH
FEATURES             Location/Qualifiers
     source          1..9382
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    47..71
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_RNA        355..430
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             2896..2913
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      2917..3169
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3270..3294
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4607..5236
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5665..6738
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGGAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6804..6998
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7234..7256)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7342..7482
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7397..7416
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7497..7514)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7668..8256)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7748..7767)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(8367..8386)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             8378..9193
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             8378..9193
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(8451..8470)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt
       61 gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt
      121 actgaattaa cgccgaattg aattcatctg cggccatacc gcgatgaaca caccgcgtct
      181 cgtccgatcc gcgaagttaa gcatcgcagg ggccagagag tattgccgtg ggtgaccagg
      241 cgagaacact gtgctgccgc aggtggcggt gtggccaagt ggcacggcac ctgtttccgg
      301 cgcaggagat cgagggttcg atccccttca ccgtcgaggt cttcaggaag acgagtttta
      361 gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc
      421 gagtcggtgc tttttttttg gtacccgggc tgcagaacta cgccctctca cacccaactt
      481 ccgactcgac cggcggtacg agcacgacct acttctactg cctgccatcg acatccgggc
      541 gggtcgctgc ctaccctgtg cgttctgcgc cctccctcgt ctcgggaggc agtgtctgac
      601 agaagctttg cgcgcagtac cccgtcaaga tgcaactcta cgcaacgttc ggcacagaag
      661 tcgccaagct ccgcgcatcg ccgcctcaag ctctcgcgct gcccgacggt gtcgtctatt
      721 acgaggcgga gaagctcgag ttgccggctt tgccagcggc ggtcaaggtt gaggtggaga
      781 cggagaaggc gggagtagcg ggggaggaca atgaggcgaa gggtgagatg gtgctggtgg
      841 agactcttac ggtggagcag gaggagattg aattgggctc gggagtcgtg cagattgagg
      901 agtcgttgct cgtcaagctg gaggtcagcg gctgatcctt ccgttcgttg caaggatcgt
      961 ctgcatgttt cgcttctctc aatgacacaa cctggagagc gctcccgtca gcgagaatcg
     1021 aggacattcc gcagctcgtg agcaagcgga ggtgcgaggc tccctcgaaa gctgcgcctc
     1081 ttcagacggc ttgttctctc ctgctctggt gggctggcct gacatgtaat gtgctccgcc
     1141 gcaagtccgt cgtcggtctc aattcgacgt tgaaagggca tagcgcaagg aagaaccctc
     1201 tgcggacatg cagaattact ggctcgcctg ctccttcgtc tactggaata agtcctgtct
     1261 cgttaaagcc ccaacgtcgt ttttcgacgt ttgtaaggcg caagaggtgc tatgggctac
     1321 gcaggaagct gagaggacat agaagtcggg ggaggaacgg cgcagagcgg cagttgcgga
     1381 agcatgagga aagcgagacg gtccagcatc tgcagcgcca atccgcaatc tcctggttga
     1441 gcctgcaccg gaagcgtcgg aacagtatgc gcagagtcga acgcaagtaa gaaagacgca
     1501 ccctcacact cgcttacttc gagccataca acggatcaaa gctgcgcgta tctcggcttg
     1561 taagggccgg aaagcaacct cggagatgga cacgtcacat caccaactta tcgatctcgg
     1621 ccgtcgacgt cgcagagagg gcgagagaag cggtgaagga gggaaacaac ccctcgagag
     1681 catgatccga ccgaatctgc agcgcaggaa gccgttacaa gcccgcctcg agcgcaggtc
     1741 gggtccagcc gggggacgaa acgcgcgagg ctgattcgtg agcgaaggaa gccgcatcga
     1801 caagttcgct cccctttgcc ctctttccca tcacccgttc tcgccttacc cgctcagaac
     1861 aacaccagat cactcacaat gccggagctc atggcgacgt cggtcgagaa gttcctcatc
     1921 gaaaagttcg actcggtctc ggacctcatg caactctcgg agggagagga atcgcgcgcg
     1981 ttctcgttcg acgtcggagg ccgcggatac gtcctccgcg tcaactcgtg tgcggacgga
     2041 ttctacaagg atcggtacgt ctaccgccat tttgcgtcgg cggcgctccc gatccccgaa
     2101 gtcctcgaca tcggagagtt ctcggaatcc ctcacgtact gtatctcgcg ccgggcgcaa
     2161 ggagtcacgc tccaagatct cccggagacg gaactcccgg cggtcctcca accggtcgcg
     2221 gaagcgatgg acgcgatcgc ggccgcggac ctctcgcaaa cgtcgggatt cggaccgttt
     2281 ggaccgcaag gaatcggaca atacacgacg tggcgcgact tcatctgtgc gatcgcggat
     2341 ccccatgtct accactggca aacggtcatg gatgacacgg tctcggcgtc ggtcgcgcaa
     2401 gcgctcgacg agctcatgct ctgggcggag gactgtccgg aggtccgcca cctcgtccac
     2461 gcggactttg gatcgaacaa cgtcctcacg gacaacggac gcatcacggc ggtcatcgac
     2521 tggtcggagg cgatgtttgg agactcgcaa tacgaggtcg cgaacatctt cttctggcgc
     2581 ccgtggctcg cgtgcatgga gcaacagacg cgctacttcg agcgccgcca cccggagctc
     2641 gcgggatcgc cgcgcctccg cgcgtacatg ctccgcatcg gcctcgacca actctaccag
     2701 tcgctcgtcg acggaaactt cgacgatgcc gcgtgggccc aaggacgctg cgacgcgatt
     2761 gtccgctcgg gagcgggaac cgtgggacgc acgcaaattg cgcggcgctc ggcggccgtc
     2821 tggacggatg gatgtgtcga agtcctcgcg gattcgggaa accggcgccc gtcgacgcgc
     2881 ccgcgggcga aagaacacca ccatcaccat cactaggatc gttcaaacat ttggcaataa
     2941 agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg
     3001 aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt
     3061 tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc
     3121 gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg ggcctggatc
     3181 ctctagagtc gacctgcagc atgcaagctt ggcagcttga gcttggatca gattgtcgtt
     3241 tcccgccttc agtttaaact atcagtgttt gacaggatat attggcgggt aaacctaaga
     3301 gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt
     3361 cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttaaag
     3421 tactttaaag tactttaaag tactttgatc caacccctcc gctgctatag tgcagtcggc
     3481 ttctgacgtt cagtgcagcc gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
     3541 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
     3601 tagaaccgga gacattacgc catgaacaag agcgccaccg ctggcctgct gggctatgcc
     3661 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
     3721 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
     3781 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
     3841 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
     3901 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
     3961 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
     4021 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
     4081 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
     4141 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
     4201 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
     4261 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
     4321 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
     4381 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg
     4441 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
     4501 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
     4561 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
     4621 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
     4681 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
     4741 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
     4801 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
     4861 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
     4921 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
     4981 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
     5041 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
     5101 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
     5161 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
     5221 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
     5281 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
     5341 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
     5401 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
     5461 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
     5521 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
     5581 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg
     5641 aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc
     5701 ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg
     5761 caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc
     5821 cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag
     5881 ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt
     5941 cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag
     6001 gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg
     6061 gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg
     6121 aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg
     6181 gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa
     6241 acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac
     6301 ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag
     6361 agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag
     6421 atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat
     6481 cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc
     6541 agatggttgt tcaagacgat ctacgaacgc agtggcggcg ccggagagtt caagaagttc
     6601 tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag
     6661 gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa
     6721 gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa
     6781 aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac
     6841 attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac
     6901 atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa
     6961 cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc
     7021 gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg
     7081 cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta
     7141 ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc
     7201 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     7261 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     7321 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
     7381 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     7441 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
     7501 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7561 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7621 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7681 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7741 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7801 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     7861 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     7921 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     7981 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     8041 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     8101 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     8161 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     8221 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     8281 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gaacaataaa
     8341 actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac
     8401 gtcttgctct aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg
     8461 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgattgtatg ggaagcccga
     8521 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga
     8581 gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat
     8641 ccgtactcct gatgatgcat ggttactcac cactgcgatc cccgggaaaa cagcattcca
     8701 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct
     8761 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg
     8821 tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga
     8881 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt
     8941 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga
     9001 ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga
     9061 tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt
     9121 tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga
     9181 tgagtttttc taatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct
     9241 ccgcgagatc atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc
     9301 ggtaacatga gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact
     9361 gatgggctgc ctgtatcgag tg
//
