LOCUS       sequence-272113-        1993 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  iFLinkC Linker cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Grawe A, Ranglack J, Weyrich A, Stein V
  TITLE     iFLinkC: an iterative functional linker cloning strategy for the 
            combinatorial assembly and recombination of linker peptides with 
            functional domains.
  JOURNAL   Nucleic Acids Res. 2020 Jan 11. pii: 5700546. doi: 
            10.1093/nar/gkz1210.
  PUBMED    31925441
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Jan 11. pii: 5700546. doi: 10.1093/nar/gkz1210."
COMMENT     Sequence Label: pL2-(GGS)3G
FEATURES             Location/Qualifiers
     source          1..1993
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     240..262
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(300..318)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             complement(451..1266)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(451..1266)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1174..1193
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1258..1277
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      1394..1981
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1883..1902
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
       61 ttatcccctg attctgtgga taaccgtatt accgccaatt ggctcgccgc agccgaacga
      121 ccgagcgcag cgagtcaccc gaagaccatt ttggacgcca gcagtgggtg gatccggagg
      181 tagcggtgga agtgggcatt gcatccagcc ccgagcaaaa ggagacccct gactagtctc
      241 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga cacgaaaggg
      301 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
      361 aggtggcctt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
      421 tcaaatatgt atccgctcat gaattaattc ttagaaaaac tcatcgagca tcaaatgaaa
      481 ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa
      541 tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc
      601 gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt
      661 atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg
      721 catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc
      781 atcaaccaaa ccgttattca ttcgtgattg cgcctgagcc agacgaaata cgcggtcgct
      841 gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca ccgccagcgc
      901 atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc
      961 tgggatcgcc gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt
     1021 cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt
     1081 ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa
     1141 tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa
     1201 atcagcatcc atgttggaat ttaatcgcgg cctagagcaa gacgtttccc gttgaatatg
     1261 gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg tgaattctca
     1321 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     1381 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     1441 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     1501 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     1561 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     1621 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     1681 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     1741 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     1801 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     1861 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     1921 gccacctctg acttgagcgt cgatttttgt gatctcgtca ggggggcgga gcctatggaa
     1981 aaacgccagc aac
//
