LOCUS       sequence-272115-        2732 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AtzR expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Silverman AD, Akova U, Alam KK, Jewett MC, Lucks JB
  TITLE     Design and Optimization of a Cell-Free Atrazine Biosensor.
  JOURNAL   ACS Synth Biol. 2020 Mar 20;9(3):671-677. doi: 
            10.1021/acssynbio.9b00388. Epub 2020 Mar 3.
  PUBMED    32078765
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00388"; journalName: "ACS Synth Biol"; date: 
            "2020-03-20- 20"; volume: "9"; issue: "3"; pages: "671-677"
COMMENT     Sequence Label: pJBL7032
FEATURES             Location/Qualifiers
     source          1..2732
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             51..73
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(1101..1119)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1115..1162
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(1180..1995)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1903..1922
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1987..2006
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      2117..2705
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2606..2625
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac
       61 tttaagaagg agatatacat atgcgcgctc aacgtcctag tgcgattcag gcaattagca
      121 ttccttcctt tctgttacgt cctccgatgc aacacctgcg ctttttgcac tacatcgacg
      181 ctgtcgctcg ctgcggatcc atccgtgctg ccgccgagca gcttcatgtt gcagcatccg
      241 cggtcaaccg tcgcgttcaa gatttagaat atgaactggg taccccaatc ttcgaacgtt
      301 taccccgcgg cgttcgcctg accgccgcgg gtgaactgtt cgtcgcgtat gcgcgtcgtc
      361 gtaatgccga tttagagcag gtgcaatcgc aaatccaaga tttgagcggc atgaaacgcg
      421 gccgtgtcac gctggccgcc tcacaagctt tagccccgga attcttacct cgcgtaattc
      481 acgcgtttca ggcacaacgc ccgggcatcg cgttcgacgt aaaggtgctg gatcgcgagc
      541 gtgcggttct tgcggtgact gattttgcgg cagatttagc tctggtgttt aatccccctg
      601 atttacgcgg tcttacggtt attgcacaag cccgccaacg tatctgtgca gttgtggcct
      661 cggaccaccc ccttgctaaa cgtacttcac ttcgcctgaa ggactgcttg gactacccat
      721 tggcccttcc agactcgtca ttgagcggcc gcaatgtctt agatgaattg tttgataaat
      781 cctcagcacg cccacgtccg caactggtca gcaattcata tgagatgatg cgcggcttcg
      841 cccgtgagac ggggggggta tctttccaaa tcgaaatcgg ggctggttct acggaggggg
      901 aagtcgccat tccgatcgac gaacgttcgc tggcctcggg gcgtgtagtt ctggttgcac
      961 tgcgcgaacg cgtacttcct gtcgcatcgg ccgcatttgc cgagttcgtc gcaggtaaat
     1021 tagcagacac gactcataac gcaacctaag tcgaccggct gctaacaaag cccgaaagga
     1081 agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa
     1141 acgggtcttg aggggttttt tgctgaaagc caattctgat tagaaaaact catcgagcat
     1201 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
     1261 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
     1321 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
     1381 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
     1441 aagcttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
     1501 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac
     1561 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
     1621 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
     1681 tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
     1741 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
     1801 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
     1861 cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
     1921 cccatataaa tcagcatcca tgttggaatt taatcgcggc ttcgagcaag acgtttcccg
     1981 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt
     2041 tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg
     2101 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     2161 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     2221 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     2281 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     2341 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     2401 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     2461 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     2521 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     2581 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     2641 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     2701 ggaaaaacgc cagcaacgcg atcccgcgaa at
//
