LOCUS       sequence-272119-        3209 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AtzB expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Silverman AD, Akova U, Alam KK, Jewett MC, Lucks JB
  TITLE     Design and Optimization of a Cell-Free Atrazine Biosensor.
  JOURNAL   ACS Synth Biol. 2020 Mar 20;9(3):671-677. doi: 
            10.1021/acssynbio.9b00388. Epub 2020 Mar 3.
  PUBMED    32078765
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00388"; journalName: "ACS Synth Biol"; date: 
            "2020-03-20- 20"; volume: "9"; issue: "3"; pages: "671-677"
COMMENT     Sequence Label: pJBL7035
FEATURES             Location/Qualifiers
     source          1..3209
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             51..73
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(1578..1596)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1592..1639
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(1657..2472)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2380..2399
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2464..2483
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      2594..3182
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3083..3102
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac
       61 tttaagaagg agatatacat atgactacta ccctgtatac gggattccat caattagtga
      121 caggtgacgt agcggggacc gttctgaatg gtgtcgatat tctggtacgt gatggggaaa
      181 ttatcggctt gggcccagat ttgcctcgca cactggcacc catcggcgtt gggcaagagc
      241 aaggcgtcga agtggttaat tgtcgcggac ttaccgcgta tccgggttta atcaatacgc
      301 accatcactt cttccaggcg tttgtccgca atcttgcacc ccttgattgg acccagttgg
      361 acgtcttagc atggcttcgt aagatctatc ccgtttttgc cttagttgat gaggattgta
      421 tttatcattc gaccgtagtc tcgatggctg agctgattaa acacggctgt acgactgcgt
      481 ttgatcacca atataactac tcgcgtcgcg ggggcccctt cctggttgac cgccagttcg
      541 atgcagccaa tttgttgggc cttcgttttc atgcaggtcg cggatgcatc acgcttccga
      601 tggccgaggg gagcactatt cccgatgcga tgcgtgagag cacagacaca ttccttgcag
      661 attgtgaacg cctggtttct cgtttccatg acccgcgccc tttcgctatg caacgtgtag
      721 ttgttgctcc atcctctcca gtgattgctt atcccgaaac ctttgtggag tcagcacgtc
      781 ttgcccgtca tttaggtgtt agtttacaca ctcatctggg ggaaggagag acacctgcga
      841 tggtcgcccg ctttggggag cgctcattag attggtgtga gaaccgtggt ttcgttggtc
      901 cagatgtatg gttagcacac ggatgggaat ttaccgcggc cgatattgcg cgtcttgccg
      961 ctaccggcac cggggtagcc cattgtcctg ccccggtctt cttggttgga gctgaggtta
     1021 cggatattcc ggcgatggca gcagctgggg tacgtgtagg gttcggtgtg gacgggcacg
     1081 cctcaaatga ctcctcgaac ctggcagagt gtattcgctt ggcgtacctt ctgcagtgtc
     1141 tgaaggccag cgagcgtcag caccctgttc ctgcgccgta cgatttctta cgtatggcta
     1201 ctcaaggggg ggcggactgc ttaaatcgtc ctgacttagg cgccttagct gtcgggcgcg
     1261 cggctgactt ttttgccgtg gatttaaacc gcattgagta tattggagcg aatcacgatc
     1321 ctcgttccct tcccgccaag gtgggattct ccggacccgt agacatgaca gttattaatg
     1381 gtaaggtagt ctggcgcaat ggcgagttcc ctggtcttga cgagatggag cttgcacgcg
     1441 ctgcggacgg agtgttccgc cgcgttattt acggagatcc gttggttgct gccttgcgtc
     1501 gcggtaccgg ggttactccc tgttaagtcg accggctgct aacaaagccc gaaaggaagc
     1561 tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg
     1621 ggtcttgagg ggttttttgc tgaaagccaa ttctgattag aaaaactcat cgagcatcaa
     1681 atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt
     1741 ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg
     1801 gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat
     1861 aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag
     1921 cttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc
     1981 actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg
     2041 atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc
     2101 cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt
     2161 tttcccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt
     2221 gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac
     2281 atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc
     2341 atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc
     2401 atataaatca gcatccatgt tggaatttaa tcgcggcttc gagcaagacg tttcccgttg
     2461 aatatggctc ataacacccc ttgtattact gtttatgtaa gcagacagtt ttattgttca
     2521 tgatgatata tttttatctt gtgcaatgta acatcagaga ttttgagaca caacgtgaga
     2581 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2641 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     2701 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     2761 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     2821 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     2881 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     2941 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     3001 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     3061 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     3121 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     3181 aaaacgccag caacgcgatc ccgcgaaat
//
