LOCUS       sequence-272163-         653 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to sense miRNA activity using fluorescence based tools. 
            hsa-miR-412-3p target in 3' UTR of of mKate. miRNA activity can be 
            measured by the repression of mKate2 relative to EBFP2 fluorescent 
            proteins . Plasmid is inherently unstable; please see Depositor 
            Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB-hsa-miR-412-3p
FEATURES             Location/Qualifiers
     source          1..653
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(96..120)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(348..367)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    413..468
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(467..486)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 caaagagaca tacgtcgagc agcacgaggt ggctgtggcc agatactgcg acctccctag
       61 caaactgggg cacaaactta attgagaatt ctaccaccca gctttcttgt acaaagtggt
      121 acgtctcaag accagaggtc tctgcttgcg gcaagacggc tagtggacca ggtgaagtac
      181 ggctagtgga ccaggtgaag tacggctagt ggaccaggtg aagtacggct agtggaccag
      241 gtgaagtcaa ccagaagaga cctgtgcttc actcctcagg tgcaggctgc ctatcagaag
      301 gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct
      361 gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg
      421 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca
      481 tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca
      541 tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg
      601 aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg agg
//
