LOCUS       sequence-272173-        3791 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcriptional unit for constitutive expression of Lucia luciferase
            in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young 
            DW, Venken KJT
  TITLE     Examining multiple cellular pathways at once using multiplex 
            hextuple luciferase assaying.
  JOURNAL   Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.
  PUBMED    31836712
REFERENCE   2  (bases 1 to 3791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-13"; volume: "10(1):5710. doi"; pages: " 
            10.1038/s41467-019-13651-y"
COMMENT     Sequence Label: hCMV-IE1:Lucia:bgH
FEATURES             Location/Qualifiers
     source          1..3791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     74..95
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     enhancer        318..621
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        622..825
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     776..796
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    1489..1713
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(1833..2421)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1913..1932)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2536..2638
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2639..3454
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             2639..3454
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2712..2731)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      join(3568..3791,1..232)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3655..3674)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggttcacgta gtgggccagc gccctgatag acggtttttc gccctttgac gttggagtcc
       61 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
      121 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
      181 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat tttaacaagc
      241 ttcgtctcag tcaggagtag ttattaatag taatcaatta cggggtcatt agttcatagc
      301 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
      361 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
      421 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      481 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      541 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      601 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
      661 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
      721 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
      781 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
      841 cagatcaagc tcaaatggag atcaaggttc tgttcgctct gatctgtatt gctgttgccg
      901 aggccaagcc gacagagata aatgaggacc tcaacattgc agccgtagcc tccaacttcg
      961 ccacaacgga cctcgagaca gacttgttta cgaattggga gactatgaac gttatatcca
     1021 ccgatactga gcaagtgaat acggacgctg accgtggcaa gctccctgga aagaaattgc
     1081 ctcctgatgt cctgcgtgaa ctcgaagcga atgcgcgtcg cgcaggttgt actcgcggtt
     1141 gcctcatatg cctgagccat atcaaatgta cacctaaaat gaagaaattt attccgggac
     1201 gttgtcatac gtatgagggt gaaaaggaaa gcgcacaggg tggcatagga gaggctatcg
     1261 ttgacatccc tgaaatacca ggttttaagg acaaggaacc attggaccag tttattgccc
     1321 aagtggacct ctgtgccgac tgcacgacgg gttgtctgaa gggtctggct aatgtccaat
     1381 gcagcgattt gctcaaaaag tggctgccgc aacgctgtac gacttttgca tccaagatcc
     1441 agggacgagt cgataagatc aagggattgg ctggagatcg gtgagcttct gtgccttcta
     1501 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     1561 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     1621 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     1681 gcaggcatgc tggggatgcg gtgggctcta tggcgcttga gacgaagctt gacacggtta
     1741 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     1801 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     1861 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     1921 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     1981 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     2041 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     2101 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     2161 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     2221 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     2281 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     2341 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     2401 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     2461 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     2521 tagatccttt tgtgatgatc ggcacgtaag aggttccaac tttcaccata atgaaataag
     2581 atcactaccg ggcgtatttt ttgagttatc gagattttca ggagctaagg aagctaaaat
     2641 gagccatatt caacgggaaa cgtcttgctc aaggccgcga ttaaattcca acatggatgc
     2701 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta
     2761 ccgattgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt
     2821 tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct
     2881 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat
     2941 cccagggaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt
     3001 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt
     3061 taacagcgat cgcgtatttc gactcgctca ggcgcaatca cgaatgaata acggtttggt
     3121 tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga
     3181 aatgcataaa cttttgccat tctcaccgga ttcagtagtc actcatggtg atttctcact
     3241 tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg
     3301 aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc
     3361 ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt
     3421 gcagtttcat ttgatgctcg atgagttttt ctgatttttt ttaaggcagt tattggtgcc
     3481 cttaaacgcc tggttgctac gcctgaataa gtgataataa gcggatgaat ggcagaaatt
     3541 cgatgataag ctgtcaaaca tgatgttacg cgccctgtag cggcgcatta agcgcggcgg
     3601 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     3661 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     3721 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     3781 attagggtga t
//
