LOCUS       sequence-272174-        3821 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcriptional unit for constitutive expression of MetLuc in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young 
            DW, Venken KJT
  TITLE     Examining multiple cellular pathways at once using multiplex 
            hextuple luciferase assaying.
  JOURNAL   Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.
  PUBMED    31836712
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-13"; volume: "10(1):5710. doi"; pages: " 
            10.1038/s41467-019-13651-y"
COMMENT     Sequence Label: hCMV-IE1:MetLuc:bgH
FEATURES             Location/Qualifiers
     source          1..3821
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    1202..1426
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(1546..2134)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1626..1645)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2249..2351
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2352..3167
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             2352..3167
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2425..2444)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3281..3736
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3368..3387)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3578..3599
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatca agctcaaatg
      541 gacatcaaag tcgtgttcac gctggtgttt tccgcgctcg tacaagctaa atccacagaa
      601 tttgacccga acattgatat tgtcggcctg gagggaaaat tcggtataac caacttggag
      661 acagatctct ttacgatttg ggagacaatg gaggtcatga taaaggcgga catagcagat
      721 acagataggg caagtaattt tgtagcaacc gaaacggacg ctaaccgtgg caaaatgccc
      781 ggaaagaagc tccctttggc cgtaataatg gagatggagg ctaatgcctt taaagccgga
      841 tgtactcgtg gctgcctgat ttgcttgagc aagatcaaat gtacagccaa aatgaaggtg
      901 tatatacccg gacggtgtca cgactatggc ggagataaaa agacgggaca ggcaggaata
      961 gtgggtgcta tagtagatat accagaaatt agcggattta aagagatggc accaatggaa
     1021 caattcattg ctcaagtaga ccgatgcgcc agctgcacga cgggctgttt gaagggattg
     1081 gcaaatgtta agtgtagcga actcctgaaa aaatggctgc ctgaccgctg tgcctccttc
     1141 gcggataaga tacagaagga ggtccataat atcaagggca tggcaggaga taggtaggct
     1201 tctgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     1261 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     1321 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     1381 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcgct tgagacgaag
     1441 cttgacacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     1501 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     1561 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     1621 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     1681 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     1741 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     1801 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     1861 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     1921 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     1981 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     2041 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     2101 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     2161 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     2221 aaggatcttc acctagatcc ttttgtgatg atcggcacgt aagaggttcc aactttcacc
     2281 ataatgaaat aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta
     2341 aggaagctaa aatgagccat attcaacggg aaacgtcttg ctcaaggccg cgattaaatt
     2401 ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag
     2461 gtgcgacaat ctaccgattg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg
     2521 gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg
     2581 aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac
     2641 tcaccactgc gatcccaggg aaaacagcat tccaggtatt agaagaatat cctgattcag
     2701 gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt
     2761 gtaattgtcc ttttaacagc gatcgcgtat ttcgactcgc tcaggcgcaa tcacgaatga
     2821 ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac
     2881 aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagta gtcactcatg
     2941 gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg
     3001 ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg
     3061 gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg
     3121 atatgaataa attgcagttt catttgatgc tcgatgagtt tttctgattt tttttaaggc
     3181 agttattggt gcccttaaac gcctggttgc tacgcctgaa taagtgataa taagcggatg
     3241 aatggcagaa attcgatgat aagctgtcaa acatgatgtt acgcgccctg tagcggcgca
     3301 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     3361 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     3421 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     3481 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc cagcgccctg atagacggtt
     3541 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     3601 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     3661 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     3721 ttaacgctta caattttaac aagcttcgtc tcagtcagga gtagttatta atagtaatca
     3781 attacggggt cattagttca tagcccatat atggagttcc g
//
