LOCUS       sequence-272176-        3976 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GBPart - RoLuc Luciferase CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young 
            DW, Venken KJT
  TITLE     Examining multiple cellular pathways at once using multiplex 
            hextuple luciferase assaying.
  JOURNAL   Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.
  PUBMED    31836712
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-13"; volume: "10(1):5710. doi"; pages: " 
            10.1038/s41467-019-13651-y"
COMMENT     Sequence Label: pRoLuc
FEATURES             Location/Qualifiers
     source          1..3976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1650..1669)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1651..1669)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1676..1693)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1676..1692)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(1712..2167)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1849..1870)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2061..2080
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(2280..2939)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2854..2873
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2940..3042)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      3157..3745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3646..3665
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3860..3876
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3860..3876
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        3892..3910
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3892..3909
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 aaagccccga gatcccctcg acctcgggac ggcgggtatc cagttgtatc gcgctctgac
       61 taacttctcc tttttgcggg aggccttgat tgatgcgcac accgaagaag ttgttagtta
      121 tgcggatata ttggaaaaca gctgccgact tgccaaatgc tatgagaatt acggcctgcg
      181 ccaaaactca gtgataagtg tttgctccga aaactcaaca atttttttct accccgtgat
      241 tgctgccctc tatatgggtg taattactgc cactgtgaat gactcttata ccgaacgcga
      301 attgcttgaa acgctcaaca tttcaaagcc ggagttggta ttttgcagta aaaaagcaat
      361 caagaacatg atggctctca aaagaaatgt aaactttata aagaaagtcg tgctcctcga
      421 ttccaaggag gatatgggcg aggcccaatg tctgagcaac tttatggctc ggtattccga
      481 accaaatttg gacgttcgga attttaaacc ccgcgacttt gatgcaaagg aacaagttgc
      541 tctcattatg tcctcttccg ggaccaccgg acttccaaaa ggagtggtac tcacgcacag
      601 aaacctctca gtaagatttg ttcactgcaa ggatccactt ttcgggacaa ggacaatacc
      661 tagtacttcc attctgagta tagtgccatt tcatcatgca ttcggaatgt tcacaacatt
      721 gtcttacttc atcgtgggct tgagggtagt actccttaag cgcttcgaag agaaattttt
      781 cctgtctact atcgagaaat accgcattcc gaccatcgtg cttgcgccac cagttatggt
      841 attcctcgcc aaatccccgt tggtagacca gtatgacttg tcctctatac gcgaggttgc
      901 caccggcggt gccccagtcg ggaccgaagt agccgttgcc gtagcgaaac ggctgaaaat
      961 cggcggcatt ctgcaaggtt atggactcac ggaaacgtgt tgtgcggtgc tcatcacgcc
     1021 gcacgacgac gtcaagaccg gctcaacagg ccgggttgcg ccatacgtac aagcaaagat
     1081 agtagatctt accacgggaa aatctctggg gcctaacaag agaggtgaac tgtgcttcaa
     1141 aagtgaaatt attatgaagg gctactttaa taataaacaa gccaccgaag aagccattga
     1201 taaggaaggg tggctccact ctggggatgt cgggtactac gacgatgatg gccacttttt
     1261 tgtcgttgat cggcttaagg agctgatcaa gtacaagggt tatcaagtcg ccccagccga
     1321 gcttgagtgg ctcctcctcc aacatcccag cattaaggac gcaggagtaa ctggggtgcc
     1381 cgatgaggca gccggcgagt tgccgggggc atgtattgtg ttgcaagaag gaaaatcatt
     1441 gaccgaacaa gagatcatag attacattgc ggaaagagtt agccccacaa aacgcatccg
     1501 agggggagta gttttcgtag acgatatacc taaaggcgcg acaggcaagt tggttcgatc
     1561 cgaattgcgc aaacttctcg cgcagaagaa atccaagttg tgagcttcga gaccttgaga
     1621 tcaatatcta aggtcctaag cggaattcac cctatagtga gtcgtattac aattcactgg
     1681 ccgtcgtttt acatgcgttt ctacaatgtt aaaattgtaa gcgttaatat tttgttaaaa
     1741 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     1801 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     1861 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     1921 ggcgctggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     1981 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     2041 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     2101 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     2161 ggcgcgtaac atcatgtttg acagcttatc atcgaatttc tgccattcat ccgcttatta
     2221 tcacttattc aggcgtagca accaggcgtt taagggcacc aataactgcc ttaaaaaaat
     2281 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca
     2341 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
     2401 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
     2461 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgacacgaa aaacatattc
     2521 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
     2581 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt
     2641 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
     2701 ccgtctttca ttgccatacg gaattccgga tgagcattca tcaggcgggc aagaatgtga
     2761 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
     2821 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
     2881 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
     2941 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
     3001 atttcattat ggtgaaagtt ggaacctctt acgtgccgat catcacaaaa ggatctaggt
     3061 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     3121 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3181 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3241 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3301 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3361 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3421 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3481 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3541 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     3601 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     3661 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     3721 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     3781 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     3841 ccgtgtcatg atgcctggac aggaaacagc tatgaccaac ggctctatgg tatttaggtg
     3901 acactataga acgaattcac tagtgattgg gcgatgagtg gtctcgaatg cctaacgaaa
     3961 tcatactcca cggggc
//
