LOCUS       sequence-272178-       13636 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multiplex luciferase reporter vector, with luciferase reporters for 
            the pathways NFKB, SMAD, FOXO, P53 and AP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13636)
  AUTHORS   Sarrion-Perdigones A, Gonzalez Y, Venken KJT
  TITLE     Rapid and Efficient Synthetic Assembly of Multiplex Luciferase 
            Reporter Plasmids for the Simultaneous Monitoring of Up to Six 
            Cellular Signaling Pathways.
  JOURNAL   Curr Protoc Mol Biol. 2020 Jun;131(1):e121. doi: 10.1002/cpmb.121.
  PUBMED    32539183
REFERENCE   2  (bases 1 to 13636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Protoc
            Mol Biol."; date: "2020-06"; volume: "131(1):e121. doi"; pages: " 
            10.1002/cpmb.121"
COMMENT     Sequence Label: MLRV2: NF-kb-SMAD-DBE-P53-AP1
FEATURES             Location/Qualifiers
     source          1..13636
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    204..252
                     /note="synthetic polyadenylation signal"
     misc_feature    266..357
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     promoter        480..511
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     polyA_signal    1485..1709
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(1817..2405)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1897..1916)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             2873..3664
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3719..4174
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3806..3825)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4016..4037
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    4188..4412
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        complement(6129..6332)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6158..6178)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6333..6636
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    6714..6762
                     /note="synthetic polyadenylation signal"
     misc_feature    6776..6867
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     promoter        6980..7011
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     polyA_signal    8696..8920
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    8933..8981
                     /note="synthetic polyadenylation signal"
     misc_feature    8995..9086
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     promoter        9247..9278
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             9312..10964
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(9378..9396)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    10972..11196
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    11209..11257
                     /note="synthetic polyadenylation signal"
     misc_feature    11271..11362
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     promoter        11510..11541
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     polyA_signal    12515..12739
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    12752..12800
                     /note="synthetic polyadenylation signal"
     misc_feature    12814..12905
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     promoter        13018..13049
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     polyA_signal    join(13603..13636,1..191)
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
       61 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
      121 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
      181 gggctctatg gcgcttggcg gagaataaaa tatctttatt ttcattacat ctgtgtgttg
      241 gttttttgtg tgaatcgata gtactaacat acgctctcca tcaaaacaaa acgaaacaaa
      301 acaaactagc aaaataggct gtccccagtg caagtgcagg tgccagaaca tttctctcgc
      361 tgcagactat gagtcaaaca gcgtgactca agttgagtca gttgactcac ttgagtcact
      421 gactcaggca tgctccctcg aggatatcaa atctggcctc ggcggccaag cttagacact
      481 agagggtata taatggaagc tcgacttcca gcttggcaat ccggtactgt tggtaaagcc
      541 accaatggct tccaaagtct acgacccaga acaaaggaag aggatgatta ctggaccgca
      601 gtggtgggca aggtgcaagc agatgaatgt actcgatagt tttatcaatt attacgactc
      661 cgaaaaacac gcggaaaatg ctgtcatatt cctgcacggg aacgccacta gcagctactt
      721 gtggcggcac gtagtccccc acatagagcc tgttgcccga tgtattattc ccgatctcat
      781 tggaatgggc aaatcaggca aatctgggaa cggttcttac cgcctgctcg atcactataa
      841 atatttgacc gcttggtttg agctgcttaa tctccctaaa aaaataatat tcgttggaca
      901 cgactggggc tcagctctgg cgttccacta cgcatacgag catcaagacc gaataaaagc
      961 aatagtacat atggagtcag ttgttgacgt catagagtca tggatgggtt ggccagatat
     1021 tgaagaggaa ttggccctca tcaagagtga ggagggggaa aaaatggtcc tggaaaataa
     1081 ttttttcgtg gaaaccttgc tcccgagtaa aataatgcga aaacttgaac ccgaagagtt
     1141 tgctgcttat ttggagcctt tcaaggaaaa gggggaagta agacgaccaa ccctttcttg
     1201 gccgagggag atacctcttg ttaagggtgg aaagcccgac gtagtccaga tagtccgaaa
     1261 ttacaacgcg tacctgagag ctagtgatga cctccctaag ttgtttatcg agtcagaccc
     1321 tgggttcttc agcaacgcaa ttgtagaagg agcgaaaaaa tttccaaata cagaatttgt
     1381 gaaggttaag gggctccact ttttgcaaga ggacgccccc gatgagatgg gaaaatacat
     1441 taaatctttc gtagagcgcg tccttaagaa tgaacagtga gcttctgtgc cttctagttg
     1501 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     1561 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     1621 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     1681 gcatgctggg gatgcggtgg gctctatggc gcttgacacg gttatccaca gaatcagggg
     1741 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     1801 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     1861 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1921 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     1981 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     2041 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     2101 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     2161 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     2221 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     2281 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     2341 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     2401 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     2461 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttgtgac
     2521 cagccaggac agaaatgcct cgacttcgct gctacccaag gttgccgggt gacgcacacc
     2581 gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta
     2641 atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg
     2701 taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat
     2761 gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag
     2821 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca ttatgaggga
     2881 agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca
     2941 tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa
     3001 gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg
     3061 gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct
     3121 ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc
     3181 taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga
     3241 gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt
     3301 tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt
     3361 tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga
     3421 gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc
     3481 gccgaaggat gtagctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt
     3541 catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgtttgg cctcgcgcgc
     3601 agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa
     3661 ataatcacta gaccaatgtt acacatatat actttagatt gatttaaaac ttcatgttac
     3721 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     3781 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     3841 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     3901 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     3961 gcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4021 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     4081 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     4141 gcgaatttta acaaaatatt aacgcttaca attttaacgg atcagcgcca tagagcccac
     4201 cgcatcccca gcatgcctgc tattgtcttc ccaatcctcc cccttgctgt cctgccccac
     4261 cccacccccc agaatagaat gacacctact cagacaatgc gatgcaattt cctcatttta
     4321 ttaggaaagg acagtgggag tggcaccttc cagggtcaag gaaggcacgg gggaggggca
     4381 aacaacagat ggctggcaac tagaaggcac agaagctcaa gccgccaggg catagttctc
     4441 atcgttggcg gcggatgtct tgtacataga tccaccaccc gccttctcca taagtgcttc
     4501 tcggagttcc tttcggctga ttttgcccgt gacgttcctc ggaatagagt caacgaatct
     4561 gacaccccct cgaagatact tgctgtggct aactctttgt gcaaggaaat catatacttc
     4621 tttagccgtc agttgggccc caggttgctt gacaacgaat ccggccggaa gttcccctgc
     4681 ttctatgtcg gggataccga ctacggccac atcgcggatc cctggatgtt ggagcaaaat
     4741 ctcctccaac tcgacaggag ccacttgata tcccttgtac ttgatcagct ctttatatct
     4801 atccacaatg taaaaatatt catcctcatc atagtagcca aaatcaccac tgtgaagcca
     4861 accatcgtcg tcaatggcct ccttggtggc ctgtggatta ttcacgtagc ctttcgtgac
     4921 catggggccc caaatgcaca actctcccac ttgatttggg ccaagcgcct ctccggtgtt
     4981 tcgatctata attttagcgg ccatgtaggg cgtcactttt cccaatgaac cggatttaaa
     5041 ttcgttgtga agagtgtgga tattagcgga tgtgctttca gtcaacccgt aaccacaccg
     5101 tatgcctggc aaattaaggc gttttacggc gatttcagct acttcttttg caagaggtgc
     5161 cgcaccacaa caaagttccg ccaaggtaga aagatcatac ttatcaacca acggtgattt
     5221 agaaagaaag aggattgtac ttgggacatt gatcacactt cgtacttcat agtcttgaat
     5281 cgccttcaaa aaaacctctt ggttgaacct ccgaagcatc acgacgcgca gccccaccat
     5341 aaaatacccc aagttaatgg aaaacccaaa ggcgtgaaag aagggaagat acgccagcac
     5401 actaactccg ggaatgagtt gggtaccaac tctcgggtca gaggcgtggg tcagccgaac
     5461 acatatattg cggtgggtct gcatgacgcc cttaggcaac ccggtagtac cactactaca
     5521 cagtatcgct gcaacctggt cgaccggatc gtaatgcagt ggtttaaagg tctgaagatt
     5581 gttatcgcta tatcttgaca taaaattatg caaggattca catccgagaa ggttttcctc
     5641 agagtccaga attatgattc ttttgatata atttgttcta tccttaacct ccaaaacctt
     5701 tggaagtatc tttcgcgtgg tgaacacaag tattggctta ctaattcctg tgaccttaca
     5761 cagttcgtct ggtatatagt cctcgtttac aggtgcaacg accatgccta tataccacgc
     5821 actaataatt ggaatgaaaa atcttttgtt gttttcagcg catattgata caacatcatt
     5881 cattttatag ccacaattct ggagactctg ccccaatttg accgtcgtgt cgaagaattc
     5941 ctgataacta agactttcgt tacccatcac gtcgaggatc gcctgcggga tgtgactatg
     6001 tttgtgaagc gcgttgtaga gcatctctcc agcggtaaaa ttcccaagcg ggtgcttagg
     6061 ctcgggccca tacacaacat tcttctctct cttcatcatt tgagcttgat ctgacggttc
     6121 actaaaccag ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc
     6181 aatggggcgg agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa
     6241 ctcccattga cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca
     6301 cgcccattga tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag
     6361 atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc
     6421 catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact
     6481 gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt
     6541 ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt
     6601 cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga
     6661 actaatgacc ccgtaattga ttactattaa taactactcc ggatcccagg gagaataaaa
     6721 tatctttatt ttcattacat ctgtgtgttg gttttttgtg tgaatcgata gtactaacat
     6781 acgctctcca tcaaaacaaa acgaaacaaa acaaactagc aaaataggct gtccccagtg
     6841 caagtgcagg tgccagaaca tttctctcgc atgcatgagg ggaatttccg gggactttcc
     6901 cgggaatttg cgggggactt tccgggaatt tcctccctcg aggatatcaa atctggcctc
     6961 ggcggccaag cttagacact agagggtata taatggaagc tcgacttcca gcttggcaat
     7021 ccggtactgt tggtaaagcc accaatggag aacatggaga acgacgagaa cattgtggtg
     7081 ggacccaagc cgttctaccc gatcgaggag ggttccgctg gcacccagct gcgcaagtac
     7141 atggagcgct acgctaagct gggtgctatc gccttcacca acgccgtgac cggtgtggat
     7201 tactcctacg ccgagtacct ggagaagtcc tgctgcctcg gaaaggccct gcagaactac
     7261 ggtctggtcg tggatggccg cattgccctc tgctcggaga actgcgagga gttcttcatt
     7321 cccgtgatcg ctggtctgtt catcggagtc ggcgttgccc caaccaacga gatctacacc
     7381 ctgcgcgagc tggtccacag cctgggtatc agcaagccga ccatcgtttt ctcgagcaag
     7441 aagggcctcg acaaggttat caccgtgcaa aaaaccgtca ccaccatcaa gaccatcgtt
     7501 atcctggatt ccaaggttga ctaccgcggt taccagtgcc tcgacacctt catcaagcgt
     7561 aacaccccac ccggcttcca agcctcctcc ttcaaaaccg tggaggttga tcgcaaggag
     7621 caggtggccc tgatcatgaa ctcctccggc tccaccggtc tgccaaaggg cgtgcagctg
     7681 acccacgaga acaccgtcac ccgcttctcg catgcccgtg atcccatcta cggcaaccag
     7741 gtgtccccag gcaccgccgt cctgaccgtc gtgcccctcc accacggctt cggcatgttc
     7801 accactctcg gctacttgat ctgcggcttc cgtgtggtga tgctgactaa gttcgacgag
     7861 gagactttcc tcaagaccct gcaagactac aagtgcactt acgtgatcct ggtgccgacc
     7921 ctgttcgcca tcctgaacaa gagcgagctg ttgaacaaat acgacctgtc gaacctggtg
     7981 gagatcgctt ccggcggcgc cccactgtcg aaggaagttg gcgaggccgt tgctcgccgc
     8041 ttcaacctgc ccggcgtccg tcagggttac ggcctgaccg agactacctc ggctatcatc
     8101 atcacccccg agggcgacga taagcccggc gcttccggca aggttgttcc actcttcaaa
     8161 gccaaggtga tcgacctgga caccaagaag tccctgggtc ccaaccgtcg cggagaagtg
     8221 tgcgtcaagg gccccatgct gatgaagggc tacgtcaaca acccagaggc caccaaggag
     8281 ttgatcgacg aggagggctg gctgcacact ggtgacatcg gttactacga cgaagagaag
     8341 cacttcttca tcgttgaccg cctgaagtcc ttgatcaagt acaagggcta ccaggttccc
     8401 cccgccgagc tcgagagcgt cctgctgcag cacccctcca ttttcgatgc cggagtggcc
     8461 ggtgtccccg atcccgtggc tggcgagctg ccaggtgccg tcgtcgtgct ggagtccggc
     8521 aagaacatga ccgagaagga agtgatggat tacgtggcct cgcaagtgtc caacgccaag
     8581 cgcctgcgcg gcggcgtgcg cttcgtggat gaggtgccca agggcctcac cggaaagatc
     8641 gacggtcgcg ctatccgcga gatcctcaag aagcccgtgg ccaagatgtg agcttctgtg
     8701 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     8761 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     8821 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     8881 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cgctgtcagg agaataaaat
     8941 atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag tactaacata
     9001 cgctctccat caaaacaaaa cgaaacaaaa caaactagca aaataggctg tccccagtgc
     9061 aagtgcaggt gccagaacat ttctctcgct gtcaggaggt ctagactgcc gtctagactt
     9121 aacagtacgt ctagactcgg ccgtctagac ttagtacgtc tagactcggt atgtctagac
     9181 tgacccgtct agacggatcc tccctcgagg atatcaaatc tggcctcggc ggccaagctt
     9241 agacactaga gggtatataa tggaagctcg acttccagct tggcaatccg gtactgttgg
     9301 taaagccacc aatggaagac gccaaaaaca taaagaaagg cccggcgcca ttctatccgc
     9361 tggaagatgg aaccgctgga gagcaactgc ataaggctat gaagagatac gccctggttc
     9421 ctggaacaat tgcttttaca gatgcacata tcgaggtgga catcacttac gctgagtact
     9481 tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata tgggctgaat acaaatcaca
     9541 gaatcgtcgt atgcagtgaa aactctcttc aattctttat gccggtgttg ggcgcgttat
     9601 ttatcggagt tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca
     9661 gtatgggcat ttcgcagcct accgtggtgt tcgtttccaa aaaggggttg caaaaaattt
     9721 tgaacgtgca aaaaaagctc ccaatcatcc aaaaaattat tatcatggat tctaaaacgg
     9781 attaccaggg atttcagtcg atgtacacgt tcgtcacatc tcatctacct cccggtttta
     9841 atgaatacga ttttgtgcca gagtccttcg atagggacaa gacaattgca ctgatcatga
     9901 actcctctgg atctactggt ctgcctaaag gtgtcgctct gcctcataga actgcctgcg
     9961 tgagattctc gcatgccaga gatcctattt ttggcaatca aatcattccg gatactgcga
    10021 ttttaagtgt tgttccattc catcacggtt ttggaatgtt tactacactc ggatatttga
    10081 tatgtggatt tcgagtcgtc ttaatgtata gatttgaaga agagctgttt ctgaggagcc
    10141 ttcaggatta caagattcaa agtgcgctgc tggtgccaac cctattctcc ttcttcgcca
    10201 aaagcactct gattgacaaa tacgatttat ctaatttaca cgaaattgct tctggtggcg
    10261 ctcccctctc taaggaagtc ggggaagcgg ttgccaagag gttccatctg ccaggtatca
    10321 ggcaaggata tgggctcact gagactacat cagctattct gattacaccc gagggggatg
    10381 ataaaccggg cgcggtcggt aaagttgttc cattttttga agcgaaggtt gtggatctgg
    10441 ataccgggaa aacgctgggc gttaatcaaa gaggcgaact gtgtgtgaga ggtcctatga
    10501 ttatgtccgg ttatgtaaac aatccggaag cgaccaacgc cttgattgac aaggatggat
    10561 ggctacattc tggagacata gcttactggg acgaagacga acacttcttc atcgttgacc
    10621 gcctgaagtc tctgattaag tacaaaggct atcaggtggc tcccgctgaa ttggaatcca
    10681 tcttgctcca acaccccaac atcttcgacg caggtgtcgc aggtcttccc gacgatgacg
    10741 ccggtgaact tcccgccgcc gttgttgttt tggagcacgg aaagacgatg acggaaaaag
    10801 agatcgtgga ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg
    10861 tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga aaaatcagag
    10921 agatcctcat aaaggccaag aagggcggaa agatcgccgt gtaatgagct tctgtgcctt
    10981 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
    11041 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
    11101 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
    11161 atagcaggca tgctggggat gcggtgggct ctatggcgct aatgggagaa taaaatatct
    11221 ttattttcat tacatctgtg tgttggtttt ttgtgtgaat cgatagtact aacatacgct
    11281 ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc cagtgcaagt
    11341 gcaggtgcca gaacatttct ctcgctgaat tcgatcaagt aaacaactat gtaaacaaga
    11401 tgcagatcaa gtaaacaact atgtaaacaa ctccgttgat caagtaaaca actatgtaaa
    11461 caatccctcg aggatatcaa atctggcctc ggcggccaag cttagacact agagggtata
    11521 taatggaagc tcgacttcca gcttggcaat ccggtactgt tggtaaagcc accaatgact
    11581 tccaaggttt atgaccccga gcaacgcaag agaatgataa ctgggcctca gtggtgggcc
    11641 agatgtaaac aaatgaatgt ccttgatagt ttcatcaatt actacgacag tgaaaagcat
    11701 gcagaaaacg ccgtaatttt tttgcatggc aacgcagcct cctcttattt gtggcgacac
    11761 gtagtcccgc atatagagcc ggtagctagg tgtattatcc ctgatctgat cggaatggga
    11821 aagagtggca agtcagggaa tggatcttat cgccttcttg accactacaa gtatctgacg
    11881 gcttggttcg aactcttgaa tttgccgaag aaaatcatct tcgtgggtca cgattgggga
    11941 gcctgtctcg cgttccatta ttcctacgag catcaggaca agataaaggc aatcgttcac
    12001 gccgagtctg tagtagatgt aatcgagtcc tgggacgaat ggccagatat tgaggaggat
    12061 atagcattga ttaaaagcga ggaaggggaa aagatggtgc tggagaacaa cttctttgtg
    12121 gaaacaatgc ttccttcaaa aatcatgaga aaactggaac ccgaggaatt tgccgcttat
    12181 ctcgaaccgt ttaaagaaaa gggagaagtc cgcaggccaa cactttcctg gccaagagaa
    12241 atcccgctcg tcaaaggcgg aaagccggat gttgtgcaaa tagtccgaaa ttacaacgcg
    12301 tacctccggg cctctgacga tctccctaaa atgtttatag aatcagatcc aggatttttt
    12361 agcaacgcta tcgtagaagg agcaaagaaa tttccaaata ccgaatttgt caaagtaaag
    12421 ggactgcatt tcagtcaaga ggacgctcca gacgagatgg gcaaatacat aaagtccttt
    12481 gtagagaggg tcctgaagaa cgaacagtga gcttctgtgc cttctagttg ccagccatct
    12541 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
    12601 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
    12661 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
    12721 gatgcggtgg gctctatggc gctgcttgga gaataaaata tctttatttt cattacatct
    12781 gtgtgttggt tttttgtgtg aatcgatagt actaacatac gctctccatc aaaacaaaac
    12841 gaaacaaaac aaactagcaa aataggctgt ccccagtgca agtgcaggtg ccagaacatt
    12901 tctctcgctg tcaggagtac agaacatgtc taagcatgct gtgccttgcc tggacttgcc
    12961 tggccttgcc ttccctcgag gatatcaaat ctggcctcgg cggccaagct tagacactag
    13021 agggtatata atggaagctc gacttccagc ttggcaatcc ggtactgttg gtaaagccac
    13081 caatggtgtt taccttggag gacttcgtgg gtgactggag gcaaactgca ggatacaatt
    13141 tggaccaggt cctcgaacag ggaggagtca gcagcctttt ccaaaacttg ggcgtgtccg
    13201 ttacccctat tcagagaatt gtcctttccg gagagaacgg attgaaaata gatatccacg
    13261 tcataatccc gtatgaagga ctgtctgggg accaaatggg tcagattgaa aaaatattca
    13321 aagtagttta tcccgttgat gatcatcact tcaaagttat ccttcattat gggactttgg
    13381 taatcgatgg agttactcct aatatgatag attactttgg ccgaccttac gaggggatcg
    13441 ctgtattcga cggcaagaaa atcaccgtca ctggaaccct ctggaacggc aacaagatta
    13501 ttgacgagcg attgatcaat ccagacggat ctttgctttt ccgcgtaacg ataaacggcg
    13561 taacagggtg gaggctctgc gaacgaatac tcgcctgagc ttctgtgcct tctagttgcc
    13621 agccatctgt tgtttg
//
