LOCUS       sequence-272185-        3401 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  6 copies of the AP-1 binding motif.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Sarrion-Perdigones A, Gonzalez Y, Venken KJT
  TITLE     Rapid and Efficient Synthetic Assembly of Multiplex Luciferase 
            Reporter Plasmids for the Simultaneous Monitoring of Up to Six 
            Cellular Signaling Pathways.
  JOURNAL   Curr Protoc Mol Biol. 2020 Jun;131(1):e121. doi: 10.1002/cpmb.121.
  PUBMED    32539183
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Protoc
            Mol Biol."; date: "2020-06"; volume: "131(1):e121. doi"; pages: " 
            10.1002/cpmb.121"
COMMENT     Sequence Label: TB:6xAP-1_RE:MiniP
FEATURES             Location/Qualifiers
     source          1..3401
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1..18
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     polyA_signal    122..170
                     /note="synthetic polyadenylation signal"
     misc_feature    184..275
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     224..243
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        398..429
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      499..508
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      503..512
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(542..561)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(543..561)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(568..585)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(568..584)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(577..599)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      725..1180
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(812..831)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1022..1043
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1258..1362
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1363..1431,1432..2223)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1581..1600)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2394..2982
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2883..2902
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3136..3153
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3270..3291
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3306..3311,3312..3329,3330..3336)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3344..3360
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3349..3371
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3368..3384
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3368..3384
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atttaggtga cactatagaa tactcaagct atgcatccaa cgcgttggga gctctcccat
       61 atggtcgacc tgcaggcggc cgcgaattca ctagtgattg ggcgatgagt ggtctcggga
      121 gaataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg aatcgatagt
      181 actaacatac gctctccatc aaaacaaaac gaaacaaaac aaactagcaa aataggctgt
      241 ccccagtgca agtgcaggtg ccagaacatt tctctcgctg cagactatga gtcaaacagc
      301 gtgactcaag ttgagtcagt tgactcactt gagtcactga ctcaggcatg ctccctcgag
      361 gatatcaaat ctggcctcgg cggccaagct tagacactag agggtatata atggaagctc
      421 gacttccagc ttggcaatcc ggtactgttg gtaaagccac caatgcgaga ccactcatcg
      481 cccaatcgaa ttcccgcggc cgccatggcg gccgggagca tgcgacgtcg ggcccaattc
      541 gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggga
      601 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
      661 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
      721 atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
      781 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
      841 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
      901 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
      961 gggccagcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     1021 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     1081 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     1141 tttaacgcga attttaacaa aatattaacg cttacaattt cctgatgcgg tattttctcc
     1201 ttacgcatct gtgcggtatt tcacaccgca tcaggtggca cttttcgggg aaatgtgcgc
     1261 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     1321 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     1381 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     1441 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     1501 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     1561 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     1621 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     1681 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     1741 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     1801 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     1861 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     1921 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     1981 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     2041 tggtttattg ctgataaatc tggagccggt gagcgtgggt cgcgcggtat cattgcagca
     2101 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     2161 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     2221 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     2281 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     2341 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2401 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2461 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2521 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     2581 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2641 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2701 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2761 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     2821 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     2881 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     2941 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3001 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3061 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3121 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     3181 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3241 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     3301 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     3361 tttcacacag gaaacagcta tgaccatgat tacgccaagc t
//
