LOCUS       sequence-27219-e         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Liu Y, Min W
  TITLE     Thioredoxin promotes ASK1 ubiquitination and degradation to inhibit 
            ASK1-mediated apoptosis in a redox activity-independent manner.
  JOURNAL   Circ Res. 2002 Jun 28. 90(12):1259-66.
  PUBMED    12089063
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Circ Res. 
            2002 Jun 28. 90(12):1259-66."
COMMENT     Sequence Label: TRX1 flag C32S
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      77..86
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             86..109
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(485..503)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(486..503)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(523..540)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    529..753
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctctctggct actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagctgcca ccatggacta caaggacgac gatgacaagg gacctaagct
      121 tggtaccgag ctcggatcca tggtgaagca gatcgagagc aagactgctt ttcaggaagc
      181 cttggacgct gcaggtgata aacttgtagt agttgacttc tcagccacgt ggagtgggcc
      241 ttgcaaaatg atcaagcctt tctttcattc cctctctgaa aagtattcca acgtgatatt
      301 ccttgaagta gatgtggatg actgtcagga tgttgcttca gagtgtgaag tcaaatgcat
      361 gccaacattc cagtttttta agaagggaca aaaggtgggt gaattttctg gagccaataa
      421 ggaaaagctt gaagccacca ttaatgaatt agtctaactc gagcatgcat ctagagggcc
      481 ctattctata gtgtcaccta aatgctagag ctcgctgatc agcctcgact gtgccttcta
      541 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
      601 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
      661 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
      721 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
//
