LOCUS       sequence-272194-         599 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to sense miRNA activity using fluorescence based tools. 
            hsa-miR-208b-5p target in 3' UTR of of mKate. miRNA activity can be 
            measured by the repression of mKate2 relative to EBFP2 fluorescent 
            proteins . Plasmid is inherently unstable; please see Depositor 
            Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB-hsa-miR-208b-5p
FEATURES             Location/Qualifiers
     source          1..599
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(88..112)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(336..355)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    401..456
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(455..474)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 catacgtcga gcagcacgag gtggctgtgg ccagatactg cgacctccct agcaaactgg
       61 ggcacaaact taattgagaa ttctaccacc cagctttctt gtacaaagtg gtacgtctca
      121 agaccagagg tctctgcttc acggtccaca taattcgagc aaaaagctta cataattcga
      181 gcaaaaagct tacataattc gagcaaaaag cttacataat tcgagcaaaa agcttcaacc
      241 agaagagacc tgtgcttcac tcctcaggtg caggctgcct atcagaaggt ggtggctggt
      301 gtggccaatg ccctggctca caaataccac tgagatcttt ttccctctgc caaaaattat
      361 ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc
      421 attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca
      481 aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg
      541 ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccc
//
