LOCUS       sequence-272198-         638 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to sense miRNA activity using fluorescence based tools. 
            hsa-miR-20a-5p target in 3' UTR of of mKate. miRNA activity can be 
            measured by the repression of mKate2 relative to EBFP2 fluorescent 
            proteins . Plasmid is inherently unstable; please see Depositor 
            Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 638)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB-hsa-miR-20a-5p
FEATURES             Location/Qualifiers
     source          1..638
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(112..136)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(364..383)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    429..484
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(483..502)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 gaatcaagga ggccgacaaa gagacatacg tcgagcagca cgaggtggct gtggccagat
       61 actgcgacct ccctagcaaa ctggggcaca aacttaattg agaattctac cacccagctt
      121 tcttgtacaa agtggtacgt ctcaagacca gaggtctctg cttagtccgt tctacctgca
      181 ctataagcac tttactacct gcactataag cactttacta cctgcactat aagcacttta
      241 ctacctgcac tataagcact ttacaaccag aagagacctg tgcttcactc ctcaggtgca
      301 ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg
      361 agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac
      421 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
      481 ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt
      541 ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag
      601 aggtcatcag tatatgaaac agccccctgc tgtccatt
//
