LOCUS       sequence-27221-e         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Liu Y, Min W
  TITLE     Thioredoxin promotes ASK1 ubiquitination and degradation to inhibit 
            ASK1-mediated apoptosis in a redox activity-independent manner.
  JOURNAL   Circ Res. 2002 Jun 28. 90(12):1259-66.
  PUBMED    12089063
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Circ Res. 
            2002 Jun 28. 90(12):1259-66."
COMMENT     Sequence Label: TRX1 His C32S C35S
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             115..132
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             136..168
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(172..180,181..195)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(588..605)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    594..798
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctctctggct actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagctggct agcgtttaaa cttaagctta ccatgggggg ttctcatcat
      121 catcatcatc atggtatggc tagcatgact ggtggacagc aaatgggtcg ggatctgtac
      181 gacgatgacg ataaggtacc taggatccag tgtggtggaa ttcgcccttg tggtaccatg
      241 gtgaagcaga tcgagagcaa gactgctttt caggaagcct tggacgctgc aggtgataaa
      301 cttgtagtag ttgacttctc agccacgtgg tctgggcctt ccaaaatgat caagcctttc
      361 tttcattccc tctctgaaaa gtattccaac gtgatattcc ttgaagtaga tgtggatgac
      421 tgtcaggatg ttgcttcaga gtgtgaagtc aaatgcatgc caacattcca gttttttaag
      481 aagggacaaa aggtgggtga attttctgga gccaataagg aaaagcttga agccaccatt
      541 aatgaattag tctaagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
      601 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
      661 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
      721 gtgtcattct attctggggg gtggggtggg caggacagca agggggagaa ttgggaagac
      781 atagcagcat gctgggga
//
