LOCUS       sequence-272210-        2312 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  iFLinkC Linker cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Grawe A, Ranglack J, Weyrich A, Stein V
  TITLE     iFLinkC: an iterative functional linker cloning strategy for the 
            combinatorial assembly and recombination of linker peptides with 
            functional domains.
  JOURNAL   Nucleic Acids Res. 2020 Jan 11. pii: 5700546. doi: 
            10.1093/nar/gkz1210.
  PUBMED    31925441
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Jan 11. pii: 5700546. doi: 10.1093/nar/gkz1210."
COMMENT     Sequence Label: pFD-FKBP12
FEATURES             Location/Qualifiers
     source          1..2312
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             192..512
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     primer_bind     559..581
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(619..637)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             complement(770..1585)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(770..1585)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1493..1512
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1577..1596
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      1713..2300
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2202..2221
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
       61 ttatcccctg attctgtgga taaccgtatt accgccaatt gagtgagctg ataccgctcg
      121 ccgcagccga acgaccgagc gcagcgagtc acccgcgaaa tacaagaaga caattttccc
      181 gcaccagcag tggggtgcag gtggaaacca tttctccagg cgatggccgt acgttcccga
      241 agcgcggtca aacatgcgta gtccattaca ccggtatgct ggaagatggc aaaaagttcg
      301 attcgtcccg tgaccgcaac aaacccttta agttcatgtt gggaaaacaa gaagttatcc
      361 gtggctggga ggaaggcgta gcgcaaatgt ccgttggaca acgtgcaaaa ttgacaatct
      421 ctcccgacta cgcgtatgga gcgaccggcc atccaggtat cattccacct cacgctacac
      481 tggtgttcga tgttgaactg ctgaaactgg aaggcattgc ataaagcccc gacagtaaaa
      541 agagaccctg actagtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
      601 aacgcgcgac acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
      661 taatggtttc ttagacgtca ggtggccttt tcggggaaat gtgcgcggaa cccctatttg
      721 tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact
      781 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt
      841 gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa
      901 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc
      961 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg
     1021 agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct
     1081 cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcca
     1141 gacgaaatac gcggtcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc
     1201 gcaggaacac cgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata
     1261 cctggaatgc tgttttccct gggatcgccg tggtgagtaa ccatgcatca tcaggagtac
     1321 ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca
     1381 tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg
     1441 catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag
     1501 cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag
     1561 acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca
     1621 gttttattgt gaattctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     1681 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     1741 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     1801 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     1861 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     1921 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     1981 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     2041 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     2101 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     2161 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     2221 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atctcgtcag
     2281 gggggcggag cctatggaaa aacgccagca ac
//
