LOCUS       sequence-272235-         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Flag-tagged human Src-S4-UD-mCherry in mammalian cells for
            structure-function studies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Spassov DS, Ruiz-Saenz A, Piple A, Moasser MM
  TITLE     A Dimerization Function in the Intrinsically Disordered N-Terminal 
            Region of Src.
  JOURNAL   Cell Rep. 2018 Oct 9;25(2):449-463.e4. doi: 
            10.1016/j.celrep.2018.09.035.
  PUBMED    30304684
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.09"; journalName: "Cell Rep"; date: 
            "2018-10-9- 9"; volume: "25"; issue: "2"; pages: "449-463.e4"
COMMENT     Sequence Label: pDEST40-2XHA-Src-S4-UD-mCherr
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             220..930
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(365..383)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     633..652
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atgggtagca acaagagcaa gcccaaggat gccagccagc ggcgccgcag cctggagccc
       61 gccgagaacg tgcacggcgc tggcgggggc gctttccccg cctcgcagac ccccagcaag
      121 ccagcctcgg ccgacggcca ccgcggcccc agcgcggcct tcgcccccgc ggccgccgag
      181 cccaagctgt tcggaggctt caactcctcg gacaccgtca tggtgagcaa gggcgaggag
      241 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
      301 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
      361 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
      421 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
      481 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
      541 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
      601 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
      661 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
      721 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag
      781 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc
      841 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
      901 accggcggca tggacgagct gtacaagtag
//
