LOCUS       sequence-272254-         578 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to sense miRNA activity using fluorescence based tools. 
            hsa_miR-218-1-3p target in 3' UTR of of mKate. miRNA activity can be
            measured by the repression of mKate2 relative to EBFP2 fluorescent 
            proteins . Plasmid is inherently unstable; please see Depositor 
            Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 578)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB-hsa-miR-218-1-3p
FEATURES             Location/Qualifiers
     source          1..578
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(109..133)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(357..376)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    422..477
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(476..495)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 tcaaggaggc cgacaaagag acatacgtcg agcagcacga ggtggctgtg gccagatact
       61 gcgacctccc tagcaaactg gggcacaaac ttaattgaga attctaccac ccagctttct
      121 tgtacaaagt ggtacgtctc aagaccagag gtctctgctt cccgtcggcc atggtgcttg
      181 acggaaccat ccatggtgct tgacggaacc atccatggtg cttgacggaa ccatccatgg
      241 tgcttgacgg aaccatcaac cagaagagac ctgtgcttca ctcctcaggt gcaggctgcc
      301 tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt
      361 tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc
      421 taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc
      481 ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt
      541 ttggcaacat atgcccatat gctggctgcc atgaacaa
//
