LOCUS       sequence-272266-        3869 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Foxo Renilla luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Sarrion-Perdigones A, Gonzalez Y, Venken KJT
  TITLE     Rapid and Efficient Synthetic Assembly of Multiplex Luciferase 
            Reporter Plasmids for the Simultaneous Monitoring of Up to Six 
            Cellular Signaling Pathways.
  JOURNAL   Curr Protoc Mol Biol. 2020 Jun;131(1):e121. doi: 10.1002/cpmb.121.
  PUBMED    32539183
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Protoc
            Mol Biol."; date: "2020-06"; volume: "131(1):e121. doi"; pages: " 
            10.1002/cpmb.121"
COMMENT     Sequence Label: 3xDBE_RE::Renilla
FEATURES             Location/Qualifiers
     source          1..3869
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..32
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     polyA_signal    1006..1230
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(1354..1942)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1434..1453)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2057..2159
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2160..2975
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             2160..2975
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2233..2252)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3089..3544
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3176..3195)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3386..3407
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    3569..3617
                     /note="synthetic polyadenylation signal"
     misc_feature    3631..3722
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     3671..3690
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 tagagggtat ataatggaag ctcgacttcc agcttggcaa tccggtactg ttggtaaagc
       61 caccaatgac ttccaaggtt tatgaccccg agcaacgcaa gagaatgata actgggcctc
      121 agtggtgggc cagatgtaaa caaatgaatg tccttgatag tttcatcaat tactacgaca
      181 gtgaaaagca tgcagaaaac gccgtaattt ttttgcatgg caacgcagcc tcctcttatt
      241 tgtggcgaca cgtagtcccg catatagagc cggtagctag gtgtattatc cctgatctga
      301 tcggaatggg aaagagtggc aagtcaggga atggatctta tcgccttctt gaccactaca
      361 agtatctgac ggcttggttc gaactcttga atttgccgaa gaaaatcatc ttcgtgggtc
      421 acgattgggg agcctgtctc gcgttccatt attcctacga gcatcaggac aagataaagg
      481 caatcgttca cgccgagtct gtagtagatg taatcgagtc ctgggacgaa tggccagata
      541 ttgaggagga tatagcattg attaaaagcg aggaagggga aaagatggtg ctggagaaca
      601 acttctttgt ggaaacaatg cttccttcaa aaatcatgag aaaactggaa cccgaggaat
      661 ttgccgctta tctcgaaccg tttaaagaaa agggagaagt ccgcaggcca acactttcct
      721 ggccaagaga aatcccgctc gtcaaaggcg gaaagccgga tgttgtgcaa atagtccgaa
      781 attacaacgc gtacctccgg gcctctgacg atctccctaa aatgtttata gaatcagatc
      841 caggattttt tagcaacgct atcgtagaag gagcaaagaa atttccaaat accgaatttg
      901 tcaaagtaaa gggactgcat ttcagtcaag aggacgctcc agacgagatg ggcaaataca
      961 taaagtcctt tgtagagagg gtcctgaaga acgaacagtg agcttctgtg ccttctagtt
     1021 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     1081 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     1141 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     1201 ggcatgctgg ggatgcggtg ggctctatgg cgctgctttg agacggtacc tgacacggtt
     1261 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     1321 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     1381 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     1441 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     1501 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     1561 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     1621 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     1681 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     1741 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     1801 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     1861 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     1921 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     1981 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     2041 ctagatcctt ttgtgatgat cggcacgtaa gaggttccaa ctttcaccat aatgaaataa
     2101 gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa
     2161 tgagccatat tcaacgggaa acgtcttgct caaggccgcg attaaattcc aacatggatg
     2221 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct
     2281 accgattgta tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg
     2341 ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc
     2401 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga
     2461 tcccagggaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg
     2521 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt
     2581 ttaacagcga tcgcgtattt cgactcgctc aggcgcaatc acgaatgaat aacggtttgg
     2641 ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag
     2701 aaatgcataa acttttgcca ttctcaccgg attcagtagt cactcatggt gatttctcac
     2761 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg
     2821 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc
     2881 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat
     2941 tgcagtttca tttgatgctc gatgagtttt tctgattttt tttaaggcag ttattggtgc
     3001 ccttaaacgc ctggttgcta cgcctgaata agtgataata agcggatgaa tggcagaaat
     3061 tcgatgataa gctgtcaaac atgatgttac gcgccctgta gcggcgcatt aagcgcggcg
     3121 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     3181 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     3241 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     3301 gattagggtg atggttcacg tagtgggcca gcgccctgat agacggtttt tcgccctttg
     3361 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     3421 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     3481 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     3541 attttaacgg taccgtctca aatgggagaa taaaatatct ttattttcat tacatctgtg
     3601 tgttggtttt ttgtgtgaat cgatagtact aacatacgct ctccatcaaa acaaaacgaa
     3661 acaaaacaaa ctagcaaaat aggctgtccc cagtgcaagt gcaggtgcca gaacatttct
     3721 ctcgctgaat tcgatcaagt aaacaactat gtaaacaaga tgcagatcaa gtaaacaact
     3781 atgtaaacaa ctccgttgat caagtaaaca actatgtaaa caatccctcg aggatatcaa
     3841 atctggcctc ggcggccaag cttagacac
//
