LOCUS       sequence-272272-        6015 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Omega destination vector, that has a CMV:ELUC:bgH in the backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6015)
  AUTHORS   Sarrion-Perdigones A, Gonzalez Y, Venken KJT
  TITLE     Rapid and Efficient Synthetic Assembly of Multiplex Luciferase 
            Reporter Plasmids for the Simultaneous Monitoring of Up to Six 
            Cellular Signaling Pathways.
  JOURNAL   Curr Protoc Mol Biol. 2020 Jun;131(1):e121. doi: 10.1002/cpmb.121.
  PUBMED    32539183
REFERENCE   2  (bases 1 to 6015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Protoc
            Mol Biol."; date: "2020-06"; volume: "131(1):e121. doi"; pages: " 
            10.1002/cpmb.121"
COMMENT     Sequence Label: Omega Destination-CMV::ELuc:bGH
FEATURES             Location/Qualifiers
     source          1..6015
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    2225..2449
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2463..2918)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2600..2621)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2812..2831
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(2973..3764)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4232..4820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4721..4740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        complement(5881..5928)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatca agctcaaatg
      541 atgaagagag agaagaatgt tgtgtatggg cccgagccta agcacccgct tgggaatttt
      601 accgctggag agatgctcta caacgcgctt cacaaacata gtcacatccc gcaggcgatc
      661 ctcgacgtga tgggtaacga aagtcttagt tatcaggaat tcttcgacac gacggtcaaa
      721 ttggggcaga gtctccagaa ttgtggctat aaaatgaatg atgttgtatc aatatgcgct
      781 gaaaacaaca aaagattttt cattccaatt attagtgcgt ggtatatagg catggtcgtt
      841 gcacctgtaa acgaggacta tataccagac gaactgtgta aggtcacagg aattagtaag
      901 ccaatacttg tgttcaccac gcgaaagata cttccaaagg ttttggaggt taaggataga
      961 acaaattata tcaaaagaat cataattctg gactctgagg aaaaccttct cggatgtgaa
     1021 tccttgcata attttatgtc aagatatagc gataacaatc ttcagacctt taaaccactg
     1081 cattacgatc cggtcgacca ggttgcagcg atactgtgta gtagtggtac taccgggttg
     1141 cctaagggcg tcatgcagac ccaccgcaat atatgtgttc ggctgaccca cgcctctgac
     1201 ccgagagttg gtacccaact cattcccgga gttagtgtgc tggcgtatct tcccttcttt
     1261 cacgcctttg ggttttccat taacttgggg tattttatgg tggggctgcg cgtcgtgatg
     1321 cttcggaggt tcaaccaaga ggtttttttg aaggcgattc aagactatga agtacgaagt
     1381 gtgatcaatg tcccaagtac aatcctcttt ctttctaaat caccgttggt tgataagtat
     1441 gatctttcta ccttggcgga actttgttgt ggtgcggcac ctcttgcaaa agaagtagct
     1501 gaaatcgccg taaaacgcct taatttgcca ggcatacggt gtggttacgg gttgactgaa
     1561 agcacatccg ctaatatcca cactcttcac aacgaattta aatccggttc attgggaaaa
     1621 gtgacgccct acatggccgc taaaattata gatcgaaaca ccggagaggc gcttggccca
     1681 aatcaagtgg gagagttgtg catttggggc cccatggtca cgaaaggcta cgtgaataat
     1741 ccacaggcca ccaaggaggc cattgacgac gatggttggc ttcacagtgg tgattttggc
     1801 tactatgatg aggatgaata tttttacatt gtggatagat ataaagagct gatcaagtac
     1861 aagggatatc aagtggctcc tgtcgagttg gaggagattt tgctccaaca tccagggatc
     1921 cgcgatgtgg ccgtagtcgg tatccccgac atagaagcag gggaacttcc ggccggattc
     1981 gttgtcaagc aacctggggc ccaactgacg gctaaagaag tatatgattt ccttgcacaa
     2041 agagttagcc acagcaagta tcttcgaggg ggtgtcagat tcgttgactc tattccgagg
     2101 aacgtcacgg gcaaaatcag ccgaaaggaa ctccgagaag cacttatgga gaaggcgggt
     2161 ggtggatcta tgtacaagac atccgccgcc aacgatgaga actatgccct ggcggcttga
     2221 gcttctgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     2281 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     2341 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     2401 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc gctgatccgt
     2461 taaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     2521 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     2581 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     2641 caacgtcaaa gggcgaaaaa ccgtctatca gggcgctggc ccactacgtg aaccatcacc
     2701 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     2761 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     2821 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     2881 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtaa catgaagttt taaatcaatc
     2941 taaagtatat atgtgtaaca ttggtctagt gattatttgc cgactacctt ggtgatctcg
     3001 cctttcacgt agtggacaaa ttcttccaac tgatctgcgc gcgaggccaa acgatcttct
     3061 tcttgtccaa gataagcctg tctagcttca agtatgacgg gctgatactg ggccggcagg
     3121 cgctccattg cccagtcggc agctacatcc ttcggcgcga ttttgccggt tactgcgctg
     3181 taccaaatgc gggacaacgt aagcactaca tttcgctcat cgccagccca gtcgggcggc
     3241 gagttccata gcgttaaggt ttcatttagc gcctcaaata gatcctgttc aggaaccgga
     3301 tcaaagagtt cctccgccgc tggacctacc aaggcaacgc tatgttctct tgcttttgtc
     3361 agcaagatag ccagatcaat gtcgatcgtg gctggctcga agatacctgc aagaatgtca
     3421 ttgcgctgcc attctccaaa ttgcagttcg cgcttagctg gataacgcca cggaatgatg
     3481 tcgtcgtgca caacaatggt gacttctaca gcgcggagaa tctcgctctc tccaggggaa
     3541 gccgaagttt ccaaaaggtc gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg
     3601 gtcaccgtaa ccagcaaatc aatatcactg tgtggcttca ggccgccatc cactgcggag
     3661 ccgtacaaat gtacggccag caacgtcggt tcgagatggc gctcgatgac gccaactacc
     3721 tctgatagtt gagtcgatac ttcggcgatc accgcttccc tcataatgtt taactttgtt
     3781 ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc
     3841 acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacccc aaaaaaacag
     3901 tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt
     3961 ctggaccagt tgcgtgagcg catacgctac ttgcattaca gcttacgaac cgaacaggct
     4021 tatgtccact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc ggcaaccttg
     4081 ggtagcagcg aagtcgaggc atttctgtcc tggctggtca caaaaggatc taggtgaaga
     4141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     4201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     4261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     4321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     4381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     4441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     4501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     4561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     4621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     4681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     4741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     4801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     4861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgtg
     4921 tcaagcgtga gacgtttgcc tggcggcagt agcgcggtgg tcccacctga ccccatgccg
     4981 aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggactcccca tgcgagagta
     5041 gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgccc
     5101 gggctaatta tggggtgtcg cccttggggt gagactctcg agtgtacaga attcaagctc
     5161 attacgtcgc tttttccggc acatcgctgt aacgtgcgac agccgattcg tattgttcgt
     5221 agaatttgcc ctgctcagct ttcaggattt cgatgcgatg gtcaccaaaa tggaactccg
     5281 gcatgttaac ggcgttgctc ggtttacggc tacgatacgt cgtgcgcagg tggctggtca
     5341 gatgattgcc atcggtgcac ttcagcgcca tcaagctctg accacacaag acgccgtcac
     5401 gcggatacag catctcgacg cacggctccc aacccgcggt tttcttttgc atcaccggac
     5461 cattcgccgg gaaattggta cccagaacct tcactttata gatcaggcaa ttaccttcca
     5521 ggctggtgtc ttggtgcgcg gtcagaacac caccgtcctc aaaaatctgg gtacgttccc
     5581 aagtgaagcc ctctggaaag gactctttaa agtaatccgg gataccgctc acgtgtttaa
     5641 tgaaggtctt agagccgtac atacagcacg gggccaggat gtcgaatgca aaaggcagcg
     5701 gaccgccctc ggtgacacga atgcgcatgt tctgcgtgcc ctcgaacggc ttgccttcac
     5761 cctcgccaac gcacttaaag tgataaccgt tcacagtacc ttccatcgtc atcttaacac
     5821 acatatcttt cttaaccaag ctcgccattg atctgcctcc tgggtttagt tcctcacctt
     5881 gtcgtattat actatgccga tatactatgc cgatgattaa ttgtcaacag gcgtctctct
     5941 ccctgggatc cggagtagtt attaatagta atcaattacg gggtcattag ttcatagccc
     6001 atatatggag ttccg
//
