LOCUS       sequence-272276-        3416 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  p53 NLuc luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Sarrion-Perdigones A, Gonzalez Y, Venken KJT
  TITLE     Rapid and Efficient Synthetic Assembly of Multiplex Luciferase 
            Reporter Plasmids for the Simultaneous Monitoring of Up to Six 
            Cellular Signaling Pathways.
  JOURNAL   Curr Protoc Mol Biol. 2020 Jun;131(1):e121. doi: 10.1002/cpmb.121.
  PUBMED    32539183
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Protoc
            Mol Biol."; date: "2020-06"; volume: "131(1):e121. doi"; pages: " 
            10.1002/cpmb.121"
COMMENT     Sequence Label: 2xP53_RE::NLuc
FEATURES             Location/Qualifiers
     source          1..3416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      16..471
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(103..122)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     313..334
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    497..545
                     /note="synthetic polyadenylation signal"
     misc_feature    559..650
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     599..618
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        763..794
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     polyA_signal    1348..1572
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(1697..2285)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1777..1796)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2400..2502
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2503..3318
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             2503..3318
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2576..2595)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gtcaaacatg atgttacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
       61 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
      121 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
      181 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
      241 gttcacgtag tgggccagcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
      301 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
      361 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
      421 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt ttaacctcga
      481 gcgtctcagc ttggagaata aaatatcttt attttcatta catctgtgtg ttggtttttt
      541 gtgtgaatcg atagtactaa catacgctct ccatcaaaac aaaacgaaac aaaacaaact
      601 agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga acatttctct cgctgtcagg
      661 agtacagaac atgtctaagc atgctgtgcc ttgcctggac ttgcctggcc ttgccttccc
      721 tcgaggatat caaatctggc ctcggcggcc aagcttagac actagagggt atataatgga
      781 agctcgactt ccagcttggc aatccggtac tgttggtaaa gccaccaatg gtgtttacct
      841 tggaggactt cgtgggtgac tggaggcaaa ctgcaggata caatttggac caggtcctcg
      901 aacagggagg agtcagcagc cttttccaaa acttgggcgt gtccgttacc cctattcaga
      961 gaattgtcct ttccggagag aacggattga aaatagatat ccacgtcata atcccgtatg
     1021 aaggactgtc tggggaccaa atgggtcaga ttgaaaaaat attcaaagta gtttatcccg
     1081 ttgatgatca tcacttcaaa gttatccttc attatgggac tttggtaatc gatggagtta
     1141 ctcctaatat gatagattac tttggccgac cttacgaggg gatcgctgta ttcgacggca
     1201 agaaaatcac cgtcactgga accctctgga acggcaacaa gattattgac gagcgattga
     1261 tcaatccaga cggatctttg cttttccgcg taacgataaa cggcgtaaca gggtggaggc
     1321 tctgcgaacg aatactcgcc tgagcttctg tgccttctag ttgccagcca tctgttgttt
     1381 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     1441 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     1501 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     1561 tgggctctat ggcgcttggc tgagacgctc gagtgacacg gttatccaca gaatcagggg
     1621 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     1681 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     1741 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1801 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     1861 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     1921 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1981 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     2041 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     2101 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     2161 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     2221 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     2281 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     2341 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttgtgat
     2401 gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact accgggcgta
     2461 ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatgagcca tattcaacgg
     2521 gaaacgtctt gctcaaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
     2581 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctaccgatt gtatgggaag
     2641 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
     2701 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
     2761 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatcccagg gaaaacagca
     2821 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg
     2881 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta
     2941 tttcgactcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt
     3001 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg
     3061 ccattctcac cggattcagt agtcactcat ggtgatttct cacttgataa ccttattttt
     3121 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
     3181 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
     3241 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg
     3301 ctcgatgagt ttttctgatt ttttttaagg cagttattgg tgcccttaaa cgcctggttg
     3361 ctacgcctga ataagtgata ataagcggat gaatggcaga aattcgatga taagct
//
