LOCUS       sequence-27228-e         613 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Beard C, Hochedlinger K, Plath K, Wutz A, Jaenisch R
  TITLE     Efficient method to generate single-copy transgenic mice by 
            site-specific integration in embryonic stem cells.
  JOURNAL   Genesis. 2006 Jan . 44(1):23-8.
  PUBMED    16400644
REFERENCE   2  (bases 1 to 613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genesis. 
            2006 Jan . 44(1):23-8."
COMMENT     Sequence Label: CAGGS-losSTOPlox-ClaI flp-in
FEATURES             Location/Qualifiers
     source          1..613
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..44
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     28..44
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        84..583
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(119..142)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     516..535
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 ttggagctcc accgcggtgg cggccgctct agaactagtg gatcccccgg gctgcaggaa
       61 ttcgtcgacc tcgaaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc
      121 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac
      181 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc
      241 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
      301 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
      361 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
      421 ctgggctcag aggctgggaa ggggtgggtc cgggggcggn ctcaggagcg ggctcagggg
      481 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac
      541 gtctgccgcg ctgatctcct cttcctcatc tccgggcctt tcgacctgca tccatctaga
      601 tctcgagcag ctg
//
