LOCUS       sequence-272283-        3422 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression of  CENP-R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   McKinley KL, Sekulic N, Guo LY, Tsinman T, Black BE, Cheeseman IM
  TITLE     The CENP-L-N Complex Forms a Critical Node in an Integrated Meshwork
            of Interactions at the Centromere-Kinetochore Interface.
  JOURNAL   Mol Cell. 2015 Dec 17;60(6):886-98. doi: 
            10.1016/j.molcel.2015.10.027. Epub 2015 Nov 19.
  PUBMED    26698661
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.10.027"; journalName: "Mol Cell"; date: 
            "2015-12-17- 17"; volume: "60"; issue: "6"; pages: "886-98"
COMMENT     Sequence Label: pKM610
FEATURES             Location/Qualifiers
     source          1..3422
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(17..22,23..39,40..45))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(116..149)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        226..317
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     242..259
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    1094..1228
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1131..1150)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1185..1204
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1349..1514
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      1704..2292
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2193..2212
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  2427..2650
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2717..3250)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 acgctacttg cattacagtt tacgaaccga acaggcttat gtcaactggg ttcgtgcctt
       61 catccgtttc cacggtgtgc gtcacccggc aaccttgggc agcagcgaag tcgccataac
      121 ttcgtatagc atacattata cgaagttatc tgtaactata acggtcctaa ggtagcgagt
      181 ttaaacacta gtatcgattc gcgacctact ccggaatatt aatagatcat ggagataatt
      241 aaaatgataa ccatctcgca aataaataag tattttactg ttttcgtaac agttttgtaa
      301 taaaaaaacc tataaatatt ccggattatt cataccgtcc caccatcggg cgcggatcca
      361 tgcctgttaa aagatcactg aagttggatg gtctgttaga agaaaattca tttgatcctt
      421 caaaaatcac aaggaagaaa agtgttataa cttattctcc aacaactgga acttgtcaaa
      481 tgagtctatt tgcttctccc acaagttctg aagagcaaaa gcacagaaat ggactatcaa
      541 atgaaaagag aaaaaaattg aatcacccca gtttaactga aagcaaagaa tctacaacaa
      601 aagacaatga tgaattcatg atgttgctat caaaagttga gaaattgtca gaagaaatca
      661 tggagataat gcaaaattta agtagtatac aggctttgga gggcagtaga gagcttgaaa
      721 atctcattgg aatctcctgt gcatcacatt tcttaaaaag agaaatgcag aaaaccaaag
      781 aactaatgac aaaagtgaat aaacaaaaac tgtttgaaaa gagtacagga cttcctcaca
      841 aagcatcacg tcatcttgac agctatgaat tccttaaagc cattttaaac tgacaattca
      901 aaggcctacg tcgacgagct cacttgtcgc ggccgctttc gaatctagag cctgcagtct
      961 cgacaagctt gtcgagaagt actagaggat cataatcagc cataccacat ttgtagaggt
     1021 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     1081 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     1141 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     1201 catcaatgta tcttatcatg tctggatctg atcactgctt gagcctagaa gatccggctg
     1261 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactatcat
     1321 aacccctagg gtatacccat ctaattggaa ccagataagt gaaatctagt tccaaactat
     1381 tttgtcattt ttaattttcg tattagctta cgacgctaca cccagttccc atctattttg
     1441 tcactcttcc ctaaataatc cttaaaaact ccatttccac ccctcccagt tcccaactat
     1501 tttgtccgcc cacaaccggt tgacttgggt caactgtcag accaagttta ctcatatata
     1561 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     1621 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     1681 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     1741 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     1801 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     1861 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     1921 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     1981 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     2041 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     2101 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     2161 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     2221 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     2281 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     2341 tttgctcaca tgttctttcc tgcgttatcc cctgattgac ttgggtcgct cttcctgtgg
     2401 atgcgcagat gccctgcgta agcgggtgtg ggcggacaat aaagtcttaa actgaacaaa
     2461 atagatctaa actatgacaa taaagtctta aactagacag aatagttgta aactgaaatc
     2521 agtccagtta tgctgtgaaa aagcatactg gacttttgtt atggctaaag caaactcttc
     2581 attttctgaa gtgcaaattg cccgtcgtat taaagagggg cgtggccaag ggcatgtaaa
     2641 gactatattc gcggcgttgt gacaatttac cgaacaactc cgcggccggg aagccgatct
     2701 cggcttgaac gaattgttag gtggcggtac ttgggtcgat atcaaagtgc atcacttctt
     2761 cccgtatgcc caactttgta tagagagcca ctgcgggatc gtcaccgtaa tctgcttgca
     2821 cgtagatcac ataagcacca agcgcgttgg cctcatgctt gaggagattg atgagcgcgg
     2881 tggcaatgcc ctgcctccgg tgctcgccgg agactgcgag atcatagata tagatctcac
     2941 tacgcggctg ctcaaacttg ggcagaacgt aagccgcgag agcgccaaca accgcttctt
     3001 ggtcgaaggc agcaagcgcg atgaatgtct tactacggag caagttcccg aggtaatcgg
     3061 agtccggctg atgttgggag taggtggcta cgtctccgaa ctcacgaccg aaaagatcaa
     3121 gagcagcccg catggatttg acttggtcag ggccgagcct acatgtgcga atgatgccca
     3181 tacttgagcc acctaacttt gttttagggc gactgccctg ctgcgtaaca tcgttgctgc
     3241 tgcgtaacat cgttgctgct ccataacatc aaacatcgac ccacggcgta acgcgcttgc
     3301 tgcttggatg cccgaggcat agactgtaca aaaaaacagt cataacaagc catgaaaacc
     3361 gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt gcgtgagcgc
     3421 at
//
