LOCUS       sequence-272286-        5636 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression of  CENP-N and CENP-L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5636)
  AUTHORS   McKinley KL, Sekulic N, Guo LY, Tsinman T, Black BE, Cheeseman IM
  TITLE     The CENP-L-N Complex Forms a Critical Node in an Integrated Meshwork
            of Interactions at the Centromere-Kinetochore Interface.
  JOURNAL   Mol Cell. 2015 Dec 17;60(6):886-98. doi: 
            10.1016/j.molcel.2015.10.027. Epub 2015 Nov 19.
  PUBMED    26698661
REFERENCE   2  (bases 1 to 5636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.10.027"; journalName: "Mol Cell"; date: 
            "2015-12-17- 17"; volume: "60"; issue: "6"; pages: "886-98"
COMMENT     Sequence Label: pKM394
FEATURES             Location/Qualifiers
     source          1..5636
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(20..25,26..42,43..48))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(119..152)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        229..320
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     regulatory      378..387
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1410..1430
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     polyA_signal    1634..1768
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1940..2031
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     regulatory      2089..2098
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3330..3464
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  3585..3750
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3940..4528
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4429..4448
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  4663..4886
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4953..5486)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 catacgctac ttgcattaca gtttacgaac cgaacaggct tatgtcaact gggttcgtgc
       61 cttcatccgt ttccacggtg tgcgtcaccc ggcaaccttg ggcagcagcg aagtcgccat
      121 aacttcgtat agcatacatt atacgaagtt atctgtaact ataacggtcc taaggtagcg
      181 agtttaaaca ctagtatcga ttcgcgacct actccggaat attaatagat catggagata
      241 attaaaatga taaccatctc gcaaataaat aagtatttta ctgttttcgt aacagttttg
      301 taataaaaaa acctataaat attccggatt attcataccg tcccaccatc gggcgcggat
      361 ccaactccta aaaaaccgcc accatggatg agactgttgc tgagttcatc aagaggacca
      421 tcttgaaaat ccccatgaat gaactgacaa caatcctgaa ggcctgggat tttttgtctg
      481 aaaatcaact gcagactgta aatttccgac agagaaagga atctgtagtt cagcacttga
      541 tccatctgtg tgaggaaaag cgtgcaagta tcagtgatgc tgccctgtta gacatcattt
      601 atatgcaatt tcatcagcac cagaaagttt gggatgtttt tcagatgagt aaaggaccag
      661 gtgaagatgt tgaccttttt gatatgaaac aatttaaaaa ttcgttcaag aaaattcttc
      721 agagagcatt aaaaaatgtg acagtcagct tcagagaaac tgaggagaat gcagtctgga
      781 ttcgaattgc ctggggaaca cagtacacaa agccaaacca gtacaaacct acctacgtgg
      841 tgtactactc ccagactccg tacgccttca cgtcctcctc catgctgagg cgcaatacac
      901 cgcttctggg tcaggcgctg acaattgcta gcaaacacca tcagattgtg aaaatggacc
      961 tgagaagtcg gtatctggac tctcttaagg ctattgtttt taaacagtat aatcagacct
     1021 ttgaaactca caactctacg acacctctac aggaaagaag ccttggacta gatataaata
     1081 tggattcaag gatcattcat gaaaacatag tagaaaaaga gagagtccaa cgaataactc
     1141 aagaaacatt tggagattat cctcaaccac aactagaatt tgcacaatat aagcttgaaa
     1201 cgaaattcaa aagtggttta aatgggagca tcttggctga gagggaagaa cccctccgat
     1261 gcctaataaa gttctctagc ccacatcttc tggaagcatt gaaatcctta gcaccagcgg
     1321 gtattgcaga tgctccactt tctccactgc tcacttgcat acccaacaag agaatgaatt
     1381 attttaaaat tagagataaa ggcagcagcc atcatcatca tcatcaccac tgagaattca
     1441 aaggcctacg tcgacgagct cacttgtcgc ggccgctttc gaatctagag cctgcagtct
     1501 cgacaagctt gtcgagaagt actagaggat cataatcagc cataccacat ttgtagaggt
     1561 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     1621 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     1681 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     1741 catcaatgta tcttatcatg tctggatctg atcactgctt gagcctagaa gatccggctg
     1801 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactatcat
     1861 aacccctagg gtatacccat ctaaggtagc gagtttaaac actagtatcg attcgcgacc
     1921 tactccggaa tattaataga tcatggagat aattaaaatg ataaccatct cgcaaataaa
     1981 taagtatttt actgttttcg taacagtttt gtaataaaaa aacctataaa tattccggat
     2041 tattcatacc gtcccaccat cgggcgcgga tccaactcct aaaaaaccgc caccatggat
     2101 tcttacagtg caccagagtc aactcctagt gcatcctcaa gacctgaaga ttactttata
     2161 ggtgccactc ctctgcagaa acgattagaa tcggtcagga agcagagttc atttatcctg
     2221 actccacctc gaaggaaaat tccccagtgt tcgcagttgc aggaagatgt tgaccctcaa
     2281 aaggttgcat tccttctgca taaacagtgg actttatata gtttaactcc cttatataaa
     2341 ttctcctata gtaatctcaa agagtattct agacttctca atgcttttat tgttgctgaa
     2401 aagcaaaaag gacttgctgt ggaagtggga gaagacttca acatcaaagt gattttttct
     2461 actctcctag gaatgaaagg aacacaaagg gacccggaag catttcttgt ccagattgtg
     2521 tcaaaatctc aattgccatc tgagaataga gaaggtaaag tgctgtggac tggctggttc
     2581 tgctgtgtat ttggagacag tcttctggag actgtttcag aagatttcac ctgtctgccc
     2641 ttattccttg caaatggagc agagtctaac acagcaataa ttggaacttg gtttcagaaa
     2701 acctttgact gttatttcag tcctttagca atcaatgcat ttaatctttc ctggatggct
     2761 gccatgtgga ctgcatgcaa aatggaccat tatgtggcta ctactgaatt tctttggtct
     2821 gtaccctgta gccctcaaag tctggacatt tctttcgcaa tacatccaga ggatgcaaaa
     2881 gctctatggg acagtgtcca caaaacacct ggggaggtta cccaggaaga agttgaccta
     2941 ttcatggatt gcctttattc acatttccat agacatttca aaattcattt atcagccaca
     3001 agattagttc gtgtttcaac atctgtagct tcagcacata ctgatggaaa aataaagatt
     3061 ctgtgtcata aataccttat tggagtgtta gcatatttga cagaactggc aatttttcaa
     3121 attgagtgag aattcaaagg cctacgtcga cgagctcact tgtcgcggcc gctttcgaat
     3181 ctagagcctg cagtctcgac aagcttgtcg agaagtacta gaggatcata atcagccata
     3241 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     3301 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     3361 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     3421 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatctgatca ctgcttgagc
     3481 ctagaagatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct
     3541 gagcaataac tatcataacc cctagggtat acccatctaa ttggaaccag ataagtgaaa
     3601 tctagttcca aactattttg tcatttttaa ttttcgtatt agcttacgac gctacaccca
     3661 gttcccatct attttgtcac tcttccctaa ataatcctta aaaactccat ttccacccct
     3721 cccagttccc aactattttg tccgcccaca accggttgac ttgggtcaac tgtcagacca
     3781 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     3841 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     3901 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     3961 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     4021 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     4081 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     4141 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     4201 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     4261 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     4321 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     4381 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     4441 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     4501 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     4561 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attgacttgg
     4621 gtcgctcttc ctgtggatgc gcagatgccc tgcgtaagcg ggtgtgggcg gacaataaag
     4681 tcttaaactg aacaaaatag atctaaacta tgacaataaa gtcttaaact agacagaata
     4741 gttgtaaact gaaatcagtc cagttatgct gtgaaaaagc atactggact tttgttatgg
     4801 ctaaagcaaa ctcttcattt tctgaagtgc aaattgcccg tcgtattaaa gaggggcgtg
     4861 gccaagggca tgtaaagact atattcgcgg cgttgtgaca atttaccgaa caactccgcg
     4921 gccgggaagc cgatctcggc ttgaacgaat tgttaggtgg cggtacttgg gtcgatatca
     4981 aagtgcatca cttcttcccg tatgcccaac tttgtataga gagccactgc gggatcgtca
     5041 ccgtaatctg cttgcacgta gatcacataa gcaccaagcg cgttggcctc atgcttgagg
     5101 agattgatga gcgcggtggc aatgccctgc ctccggtgct cgccggagac tgcgagatca
     5161 tagatataga tctcactacg cggctgctca aacttgggca gaacgtaagc cgcgagagcg
     5221 ccaacaaccg cttcttggtc gaaggcagca agcgcgatga atgtcttact acggagcaag
     5281 ttcccgaggt aatcggagtc cggctgatgt tgggagtagg tggctacgtc tccgaactca
     5341 cgaccgaaaa gatcaagagc agcccgcatg gatttgactt ggtcagggcc gagcctacat
     5401 gtgcgaatga tgcccatact tgagccacct aactttgttt tagggcgact gccctgctgc
     5461 gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg cgcttgctgc
     5521 ttggatgccc gaggcataga ctgtacaaaa aaacagtcat aacaagccat gaaaaccgcc
     5581 actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg tgagcg
//
