LOCUS       sequence-272287-         569 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to sense miRNA activity using fluorescence based tools. 
            hsa-miR-27b-5p target in 3' UTR of of mKate. miRNA activity can be 
            measured by the repression of mKate2 relative to EBFP2 fluorescent 
            proteins . Plasmid is inherently unstable; please see Depositor 
            Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB-hsa-miR-27b-5p
FEATURES             Location/Qualifiers
     source          1..569
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(113..137)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(361..380)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    426..481
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(480..499)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 agaatcaagg aggccgacaa agagacatac gtcgagcagc acgaggtggc tgtggccaga
       61 tactgcgacc tccctagcaa actggggcac aaacttaatt gagaattcta ccacccagct
      121 ttcttgtaca aagtggtacg tctcaagacc agaggtctct gcttagcaag cggttcacca
      181 atcagctaag ctctgttcac caatcagcta agctctgttc accaatcagc taagctctgt
      241 tcaccaatca gctaagctct caaccagaag agacctgtgc ttcactcctc aggtgcaggc
      301 tgcctatcag aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga
      361 tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc
      421 tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc
      481 actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta
      541 gagtttggca acatatgccc atatgctgg
//
