LOCUS       sequence-272288-        5318 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression of  CENP-P and CENP-O.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5318)
  AUTHORS   McKinley KL, Sekulic N, Guo LY, Tsinman T, Black BE, Cheeseman IM
  TITLE     The CENP-L-N Complex Forms a Critical Node in an Integrated Meshwork
            of Interactions at the Centromere-Kinetochore Interface.
  JOURNAL   Mol Cell. 2015 Dec 17;60(6):886-98. doi: 
            10.1016/j.molcel.2015.10.027. Epub 2015 Nov 19.
  PUBMED    26698661
REFERENCE   2  (bases 1 to 5318)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.10.027"; journalName: "Mol Cell"; date: 
            "2015-12-17- 17"; volume: "60"; issue: "6"; pages: "886-98"
COMMENT     Sequence Label: pKM412
FEATURES             Location/Qualifiers
     source          1..5318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(28..33,34..50,51..56))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(127..160)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        237..328
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             374..394
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     polyA_signal    1474..1608
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1780..1871
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    3001..3135
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  3256..3421
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3611..4199
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4100..4119
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  4334..4557
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4624..5157)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 cgtgagcgca tacgctactt gcattacagt ttacgaaccg aacaggctta tgtcaactgg
       61 gttcgtgcct tcatccgttt ccacggtgtg cgtcacccgg caaccttggg cagcagcgaa
      121 gtcgccataa cttcgtatag catacattat acgaagttat ctgtaactat aacggtccta
      181 aggtagcgag tttaaacact agtatcgatt cgcgacctac tccggaatat taatagatca
      241 tggagataat taaaatgata accatctcgc aaataaataa gtattttact gttttcgtaa
      301 cagttttgta ataaaaaaac ctataaatat tccggattat tcataccgtc ccaccatcgg
      361 gcgcggatcc atgcatcatc atcatcatca tcatggcagc ggcagcatgg atgccgaact
      421 ggctgaagtc cgtgctctgc aagctgagat cgctgctctg cgtcgtgcct gtgaagatcc
      481 tccggctcct tgggaagaaa aatcccgtgt ccagaaatca tttcaggcca ttcatcagtt
      541 taacctggag gggtggaaaa gctctaaaga cctgaaaaac cagctgggtc atctggaatc
      601 agagctgtcg tttctgtcta ccctgaccgg cattaacatt cgtaaccact caaaacaaac
      661 cgaggacctg acctctacag agatgacgga gaaatccatc cgtaaagtgc tgcagcgcca
      721 tcgtctgtct ggaaattgcc acatggtgac ctttcaactg gagttccaaa tcctggagat
      781 tcagaacaaa gaacgcctgt ctagtgctgt tactgatctg aacatcatca tggagccaac
      841 cgaatgctcc gaactgagcg aatttgttag ccgtgccgaa gagcgtaaag acctgttcat
      901 gttttttcgt tcactgcatt tttttgtgga atggttcgag tatcgtaaac gcaccttcaa
      961 acatctgaaa gagaaatatc ctgacgccgt gtatctgtcc gaaggccctt caagttgtag
     1021 catgggtatc cgctcggcct ctcgtccggg gttcgaactg gttattgtgt ggcgtattca
     1081 aatcgatgag gacggcaaag tgttcccgaa actggatctg ctgaccaaag ttcctcaacg
     1141 tgccctggag ctggataaaa atcgtgccat cgaaaccgct cctctgtctt tccgtaccct
     1201 ggttggtctg ctgggtattg aagcagccct ggaaagcctg attaaaagtc tgtgtgccga
     1261 ggagaacaac taagaattca aaggcctacg tcgacgagct cacttgtcgc ggccgctttc
     1321 gaatctagag cctgcagtct cgacaagctt gtcgagaagt actagaggat cataatcagc
     1381 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     1441 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     1501 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     1561 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatctg atcactgctt
     1621 gagcctagaa gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
     1681 cgctgagcaa taactatcat aacccctagg gtatacccat ctaaggtagc gagtttaaac
     1741 actagtatcg attcgcgacc tactccggaa tattaataga tcatggagat aattaaaatg
     1801 ataaccatct cgcaaataaa taagtatttt actgttttcg taacagtttt gtaataaaaa
     1861 aacctataaa tattccggat tattcatacc gtcccaccat cgggcgcgga tccatggaac
     1921 aggcaaatcc gctgcgccca gacggtgagt caaaaggtgg tgtgctggct catctggaac
     1981 gcctggaaac acaggttagc cgtagtcgta aacagagcga agaactgcag tcagtacaag
     2041 ctcaggaggg tgccctgggt acaaaaatcc acaaactgcg tcgcctgcgt gatgaactgc
     2101 gtgctgttgt ccgtcatcgt cgtgccagtg ttaaagcgtg tatcgccaac gttgaaccga
     2161 atcagacagt cgagatcaat gaacaagaag cgctggagga aaaactggag aacgtcaaag
     2221 ccattctgca agcctatcac tttaccggtc tgtcagggaa actgacctct cgtggggtgt
     2281 gtgtgtgtat tagtaccgcc ttcgagggaa atctgctgga ctcctatttc gttgacctgg
     2341 tgatccaaaa accactgcgc atccaccatc attcagtgcc ggtctttatt ccgctggaag
     2401 agattgccgc caaatatctg caaaccaaca tccaacattt tctgttcagc ctgtgtgagt
     2461 atctgaatgc ctatagcggg cgtaaatatc aggccgatcg cctgcaaagc gattttgctg
     2521 ctctgctgac gggtcctctg cagcgtaacc ctctgtgtaa tctgctgtcc ttcacctata
     2581 aactggaccc tggtggtcag agttttccgt tttgtgctcg tctgctgtat aaagacctga
     2641 ccgccacact gcctacagat gttaccgtga catgccaggg agttgaagtc ctgtctacca
     2701 gctgggaaga acaacgtgcc tcacatgaaa ccctgttctg taccaaaccg ctgcatcagg
     2761 tgtttgccag ctttactcgt aaaggcgaga aactggacat gagtctggtg tcctaagtcg
     2821 acgagctcac ttgtcgcggc cgctttcgaa tctagagcct gcagtctcga caagcttgtc
     2881 gagaagtact agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta
     2941 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     3001 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     3061 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     3121 tatcatgtct ggatctgatc actgcttgag cctagaagat ccggctgcta acaaagcccg
     3181 aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctatcataac ccctagggta
     3241 tacccatcta attggaacca gataagtgaa atctagttcc aaactatttt gtcattttta
     3301 attttcgtat tagcttacga cgctacaccc agttcccatc tattttgtca ctcttcccta
     3361 aataatcctt aaaaactcca tttccacccc tcccagttcc caactatttt gtccgcccac
     3421 aaccggttga cttgggtcaa ctgtcagacc aagtttactc atatatactt tagattgatt
     3481 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     3541 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     3601 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3661 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     3721 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     3781 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     3841 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     3901 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     3961 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     4021 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     4081 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4141 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     4201 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     4261 tctttcctgc gttatcccct gattgacttg ggtcgctctt cctgtggatg cgcagatgcc
     4321 ctgcgtaagc gggtgtgggc ggacaataaa gtcttaaact gaacaaaata gatctaaact
     4381 atgacaataa agtcttaaac tagacagaat agttgtaaac tgaaatcagt ccagttatgc
     4441 tgtgaaaaag catactggac ttttgttatg gctaaagcaa actcttcatt ttctgaagtg
     4501 caaattgccc gtcgtattaa agaggggcgt ggccaagggc atgtaaagac tatattcgcg
     4561 gcgttgtgac aatttaccga acaactccgc ggccgggaag ccgatctcgg cttgaacgaa
     4621 ttgttaggtg gcggtacttg ggtcgatatc aaagtgcatc acttcttccc gtatgcccaa
     4681 ctttgtatag agagccactg cgggatcgtc accgtaatct gcttgcacgt agatcacata
     4741 agcaccaagc gcgttggcct catgcttgag gagattgatg agcgcggtgg caatgccctg
     4801 cctccggtgc tcgccggaga ctgcgagatc atagatatag atctcactac gcggctgctc
     4861 aaacttgggc agaacgtaag ccgcgagagc gccaacaacc gcttcttggt cgaaggcagc
     4921 aagcgcgatg aatgtcttac tacggagcaa gttcccgagg taatcggagt ccggctgatg
     4981 ttgggagtag gtggctacgt ctccgaactc acgaccgaaa agatcaagag cagcccgcat
     5041 ggatttgact tggtcagggc cgagcctaca tgtgcgaatg atgcccatac ttgagccacc
     5101 taactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctgc gtaacatcgt
     5161 tgctgctcca taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc
     5221 gaggcataga ctgtacaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt
     5281 taccaccgct gcgttcggtc aaggttctgg accagttg
//
