LOCUS       sequence-272299-        5658 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expresion of CENP-L and CENP-N.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5658)
  AUTHORS   McKinley KL, Sekulic N, Guo LY, Tsinman T, Black BE, Cheeseman IM
  TITLE     The CENP-L-N Complex Forms a Critical Node in an Integrated Meshwork
            of Interactions at the Centromere-Kinetochore Interface.
  JOURNAL   Mol Cell. 2015 Dec 17;60(6):886-98. doi: 
            10.1016/j.molcel.2015.10.027. Epub 2015 Nov 19.
  PUBMED    26698661
REFERENCE   2  (bases 1 to 5658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.10.027"; journalName: "Mol Cell"; date: 
            "2015-12-17- 17"; volume: "60"; issue: "6"; pages: "886-98"
COMMENT     Sequence Label: pDK580
FEATURES             Location/Qualifiers
     source          1..5658
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(50..55,56..72,73..78))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(149..182)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        259..350
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     regulatory      408..417
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    1649..1783
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1955..2046
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             2113..2133
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     polyA_signal    3363..3497
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  3618..3783
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3973..4561
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4462..4481
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  4696..4919
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4986..5519)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gtttacgaac
       61 cgaacaggct tatgtcaact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc
      121 ggcaaccttg ggcagcagcg aagtcgccat aacttcgtat agcatacatt atacgaagtt
      181 atctgtaact ataacggtcc taaggtagcg agtttaaaca ctagtatcga ttcgcgacct
      241 actccggaat attaatagat catggagata attaaaatga taaccatctc gcaaataaat
      301 aagtatttta ctgttttcgt aacagttttg taataaaaaa acctataaat attccggatt
      361 attcataccg tcccaccatc gggcgcggat ccaactccta aaaaaccgcc accatggatt
      421 cttacagtgc accagagtca actcctagtg catcctcaag acctgaagat tactttatag
      481 gtgccactcc tctgcagaaa cgattagaat cggtcaggaa gcagagttca tttatcctga
      541 ctccacctcg aaggaaaatt ccccagtgtt cgcagttgca ggaagatgtt gaccctcaaa
      601 aggttgcatt ccttctgcat aaacagtgga ctttatatag tttaactccc ttatataaat
      661 tctcctatag taatctcaaa gagtattcta gacttctcaa tgcttttatt gttgctgaaa
      721 agcaaaaagg acttgctgtg gaagtgggag aagacttcaa catcaaagtg attttttcta
      781 ctctcctagg aatgaaagga acacaaaggg acccggaagc atttcttgtc cagattgtgt
      841 caaaatctca attgccatct gagaatagag aaggtaaagt gctgtggact ggctggttct
      901 gctgtgtatt tggagacagt cttctggaga ctgtttcaga agatttcacc tgtctgccct
      961 tattccttgc aaatggagca gagtctaaca cagcaataat tggaacttgg tttcagaaaa
     1021 cctttgactg ttatttcagt cctttagcaa tcaatgcatt taatctttcc tggatggctg
     1081 ccatgtggac tgcatgcaaa atggaccatt atgtggctac tactgaattt ctttggtctg
     1141 taccctgtag ccctcaaagt ctggacattt ctttcgcaat acatccagag gatgcaaaag
     1201 ctctatggga cagtgtccac aaaacacctg gggaggttac ccaggaagaa gttgacctat
     1261 tcatggattg cctttattca catttccata gacatttcaa aattcattta tcagccacaa
     1321 gattagttcg tgtttcaaca tctgtagctt cagcacatac tgatggaaaa ataaagattc
     1381 tgtgtcataa ataccttatt ggagtgttag catatttgac agaactggca atttttcaaa
     1441 ttgagtgaga attcaaaggc ctacgtcgac gagctcactt gtcgcggccg ctttcgaatc
     1501 tagagcctgc agtctcgaca agcttgtcga gaagtactag aggatcataa tcagccatac
     1561 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     1621 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     1681 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     1741 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctgatcac tgcttgagcc
     1801 tagaagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
     1861 agcaataact atcataaccc ctagggtata cccatctaag gtagcgagtt taaacactag
     1921 tatcgattcg cgacctactc cggaatatta atagatcatg gagataatta aaatgataac
     1981 catctcgcaa ataaataagt attttactgt tttcgtaaca gttttgtaat aaaaaaacct
     2041 ataaatattc cggattattc ataccgtccc accatcgggc gcggatccaa ctcctaaaaa
     2101 accgccacca tgcatcatca tcatcatcac cacggcagca gcatggatga gactgttgct
     2161 gagttcatca agaggaccat cttgaaaatc cccatgaatg aactgacaac aatcctgaag
     2221 gcctgggatt ttttgtctga aaatcaactg cagactgtaa atttccgaca gagaaaggaa
     2281 tctgtagttc agcacttgat ccatctgtgt gaggaaaagc gtgcaagtat cagtgatgct
     2341 gccctgttag acatcattta tatgcaattt catcagcacc agaaagtttg ggatgttttt
     2401 cagatgagta aaggaccagg tgaagatgtt gacctttttg atatgaaaca atttaaaaat
     2461 tcgttcaaga aaattcttca gagagcatta aaaaatgtga cagtcagctt cagagaaact
     2521 gaggagaatg cagtctggat tcgaattgcc tggggaacac agtacacaaa gccaaaccag
     2581 tacaaaccta cctacgtggt gtactactcc cagactccgt acgccttcac gtcctcctcc
     2641 atgctgaggc gcaatacacc gcttctgggt caggcgctga caattgctag caaacaccat
     2701 cagattgtga aaatggacct gagaagtcgg tatctggact ctcttaaggc tattgttttt
     2761 aaacagtata atcagacctt tgaaactcac aactctacga cacctctaca ggaaagaagc
     2821 cttggactag atataaatat ggattcaagg atcattcatg aaaacatagt agaaaaagag
     2881 agagtccaac gaataactca agaaacattt ggagattatc ctcaaccaca actagaattt
     2941 gcacaatata agcttgaaac gaaattcaaa agtggtttaa atgggagcat cttggctgag
     3001 agggaagaac ccctccgatg cctaataaag ttctctagcc cacatcttct ggaagcattg
     3061 aaatccttag caccagcggg tattgcagat gctccacttt ctccactgct cacttgcata
     3121 cccaacaaga gaatgaatta ttttaaaatt agagataaat aagaattcaa aggcctacgt
     3181 cgacgagctc acttgtcgcg gccgctttcg aatctagagc ctgcagtctc gacaagcttg
     3241 tcgagaagta ctagaggatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     3301 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     3361 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     3421 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     3481 cttatcatgt ctggatctga tcactgcttg agcctagaag atccggctgc taacaaagcc
     3541 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactatcata acccctaggg
     3601 tatacccatc taattggaac cagataagtg aaatctagtt ccaaactatt ttgtcatttt
     3661 taattttcgt attagcttac gacgctacac ccagttccca tctattttgt cactcttccc
     3721 taaataatcc ttaaaaactc catttccacc cctcccagtt cccaactatt ttgtccgccc
     3781 acaaccggtt gacttgggtc aactgtcaga ccaagtttac tcatatatac tttagattga
     3841 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     3901 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     3961 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     4021 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     4081 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     4141 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     4201 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     4261 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     4321 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     4381 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     4441 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     4501 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     4561 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     4621 gttctttcct gcgttatccc ctgattgact tgggtcgctc ttcctgtgga tgcgcagatg
     4681 ccctgcgtaa gcgggtgtgg gcggacaata aagtcttaaa ctgaacaaaa tagatctaaa
     4741 ctatgacaat aaagtcttaa actagacaga atagttgtaa actgaaatca gtccagttat
     4801 gctgtgaaaa agcatactgg acttttgtta tggctaaagc aaactcttca ttttctgaag
     4861 tgcaaattgc ccgtcgtatt aaagaggggc gtggccaagg gcatgtaaag actatattcg
     4921 cggcgttgtg acaatttacc gaacaactcc gcggccggga agccgatctc ggcttgaacg
     4981 aattgttagg tggcggtact tgggtcgata tcaaagtgca tcacttcttc ccgtatgccc
     5041 aactttgtat agagagccac tgcgggatcg tcaccgtaat ctgcttgcac gtagatcaca
     5101 taagcaccaa gcgcgttggc ctcatgcttg aggagattga tgagcgcggt ggcaatgccc
     5161 tgcctccggt gctcgccgga gactgcgaga tcatagatat agatctcact acgcggctgc
     5221 tcaaacttgg gcagaacgta agccgcgaga gcgccaacaa ccgcttcttg gtcgaaggca
     5281 gcaagcgcga tgaatgtctt actacggagc aagttcccga ggtaatcgga gtccggctga
     5341 tgttgggagt aggtggctac gtctccgaac tcacgaccga aaagatcaag agcagcccgc
     5401 atggatttga cttggtcagg gccgagccta catgtgcgaa tgatgcccat acttgagcca
     5461 cctaactttg ttttagggcg actgccctgc tgcgtaacat cgttgctgct gcgtaacatc
     5521 gttgctgctc cataacatca aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc
     5581 ccgaggcata gactgtacaa aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc
     5641 gttaccaccg ctgcgttc
//
