LOCUS       sequence-272310-        6397 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression of CENP-C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6397)
  AUTHORS   McKinley KL, Sekulic N, Guo LY, Tsinman T, Black BE, Cheeseman IM
  TITLE     The CENP-L-N Complex Forms a Critical Node in an Integrated Meshwork
            of Interactions at the Centromere-Kinetochore Interface.
  JOURNAL   Mol Cell. 2015 Dec 17;60(6):886-98. doi: 
            10.1016/j.molcel.2015.10.027. Epub 2015 Nov 19.
  PUBMED    26698661
REFERENCE   2  (bases 1 to 6397)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.10.027"; journalName: "Mol Cell"; date: 
            "2015-12-17- 17"; volume: "60"; issue: "6"; pages: "886-98"
COMMENT     Sequence Label: pKM568
FEATURES             Location/Qualifiers
     source          1..6397
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(57..62,63..79,80..85))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(156..189)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        266..357
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     282..299
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             400..1053
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     1011..1033
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             1060..1083
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     polyA_signal    4109..4243
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4146..4165)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4200..4219
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  4364..4529
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      4719..5307
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5208..5227
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  5442..5665
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(5732..6265)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctacttg cattacagtt
       61 tacgaaccga acaggcttat gtcaactggg ttcgtgcctt catccgtttc cacggtgtgc
      121 gtcacccggc aaccttgggc agcagcgaag tcgccataac ttcgtatagc atacattata
      181 cgaagttatc tgtaactata acggtcctaa ggtagcgagt ttaaacacta gtatcgattc
      241 gcgacctact ccggaatatt aatagatcat ggagataatt aaaatgataa ccatctcgca
      301 aataaataag tattttactg ttttcgtaac agttttgtaa taaaaaaacc tataaatatt
      361 ccggattatt cataccgtcc caccatcggg cgcggatcta tgtcccctat actaggttat
      421 tggaaaatta agggccttgt gcaacccact cgacttcttt tggaatatct tgaagaaaaa
      481 tatgaagagc atttgtatga gcgcgatgaa ggtgataaat ggcgaaacaa aaagtttgaa
      541 ttgggtttgg agtttcccaa tcttccttat tatattgatg gtgatgttaa attaacacag
      601 tctatggcca tcatacgtta tatagctgac aagcacaaca tgttgggtgg ttgtccaaaa
      661 gagcgtgcag agatttcaat gcttgaagga gcggttttgg atattagata cggtgtttcg
      721 agaattgcat atagtaaaga ctttgaaact ctcaaagttg attttcttag caagctacct
      781 gaaatgctga aaatgttcga agatcgttta tgtcataaaa catatttaaa tggtgatcat
      841 gtaacccatc ctgacttcat gttgtatgac gctcttgatg ttgttttata catggaccca
      901 atgtgcctgg atgcgttccc aaaattagtt tgttttaaaa aacgtattga agctatccca
      961 caaattgata agtacttgaa atccagcaag tatatagcat ggcctttgca gggctggcaa
     1021 gccacgtttg gtggtggcga ccatcctcca aaatcggatc tggaagttct gttccagggg
     1081 cccctgggat ccatggcagc aagtggtctg gatcacctga aaaacggcta tcgtcgtcgc
     1141 ttctgtcgcc caagtcgtgc tcgtgacatt aacaccgaac agggccagaa tgtgctggaa
     1201 atcctgcagg actgctttga agagaaaagc ctggcgaacg attttagcac caattccaca
     1261 aaaagtgtgc cgaatagtac ccgtaaaatc aaagacacgt gtatccagtc tccgtccaaa
     1321 gaatgccaaa aaagccaccc gaaaagtgta ccagtgagta gcaaaaaaaa agaggcctcg
     1381 ctgcaatttg ttgtcgaacc gagcgaagca accaaccgta gtgttcaagc ccatgaagtt
     1441 catcagaaaa tcctggcgac ggatgtttca agcaaaaaca ccccggactc caaaaaaatc
     1501 agcagccgta acattaatga ccaccactct gaagcggatg aagagttcta tctgagcgtg
     1561 ggttctccta gcgttctgct ggacgccaaa acctctgtta gccaaaacgt gattccgagt
     1621 agcgccaaaa aacgtgagac atataccttc gagaatagcg tcaacatgct gccgtcatca
     1681 acagaagtgt cggtcaaaac gaaaaaacgc ctgaatttcg atgacaaagt gatgctgaaa
     1741 aaaatcgaga tcgataataa agtctctgac gaggaggata aaacctctga aggacaggag
     1801 cgtaaacctt ctggttcatc tcagaaccgt atccgtgata gcgaatatga gattcaacgt
     1861 caggccaaaa aatcctttag cacgctgttt ctggagacag tgaaacgtaa aagcgagtct
     1921 agcccgattg ttcgtcacgc cgctacagca cctccacaca gttgtccacc ggatgatacc
     1981 aaactaatcg aagacgaatt catcattgac gagagcgacc agtcatttgc cagtcgttct
     2041 tggattacca ttcctcgtaa agccggttca ctgaaacaac gtaccattag cccggcagaa
     2101 tcaacagcac tgttccaagg ccgtaaaagt cgtgaaaaac accacaatat cctgccgaaa
     2161 acactggcca acgataaaca cagccacaaa ccacacccgg tcgagacaag tcaaccatca
     2221 gacaaaaccg tactggacac ctcttatgcc ctgattgacg agacagtcaa caactatcgt
     2281 agtaccaaat atgaaatgta tagtaaaaat gccgaaaaac cgagtcgtag caaacgtacc
     2341 atcaaacaaa aacagcgccg taaattcatg gcaaaaccgg ccgaagaaca gctggatgtt
     2401 gggcagagca aagatgagaa cattcacacg tcccatatta cccaagacga gtttcagcgt
     2461 aacagtgacc gtaacatgga agaacacgaa gaaatgggca acgattgtgt gtcgaaaaaa
     2521 caaatgccgc cggtgggttc aaaaaaatcg agtacccgta aagacaaaga agagagcaaa
     2581 aaaaaacgct ttagcagtga gtccaaaaac aaactggtgc cggaagaagt gacttcaacc
     2641 gttaccaaaa gccgccgtat tagtcgtcgt cctagcgatt ggtgggttgt gaaaagcgaa
     2701 gaaagcccgg tgtatagcaa ttctagcgtc cgtaatgagc tgcctatgca ccataacagt
     2761 tcccgtaaaa gcaccaaaaa aaccaaccag tctagcaaaa acattcgtaa aaaaacgatc
     2821 cctctgaaac gccagaaaac cgccacaaaa ggcaaccaac gtgttcagaa attcctgaac
     2881 gccgaaggat caggaggcat tgttgggcac gatgagatta gtcgctgctc cctgtcagaa
     2941 cctctggaat ccgatgaagc cgatctggct aaaaaaaaaa acctggactg ctcccgttct
     3001 acccgtagca gtaaaaatga ggataacatc atgaccgccc agaatgtccc actgaaacct
     3061 cagacaagcg gttatacctg taacattccg accgaaagca atctggacag cggtgagcac
     3121 aaaacaagtg tcctggaaga gtctggtcct agtcgtctga ataacaatta tctgatgtcc
     3181 gggaaaaatg atgtggacga tgaggaagtt cacggtagta gcgacgattc aaaacagagt
     3241 aaagtcatcc ctaaaaatcg tattcaccat aaactggtgc tgccgagtaa tactccgaac
     3301 gttcgtcgta caaaacgtac acgcctgaaa cctctggaat attggcgtgg tgaacgtatc
     3361 gactaccagg gtcgtccatc tggtggtttt gtaatcagcg gtgtgctgag tcctgatacg
     3421 attagcagca aacgtaaagc caaagagaac atcggcaaag tcaacaaaaa atccaacaaa
     3481 aaacgcatct gcctggataa cgatgagcgt aaaacgaatc tgatggtgaa tctgggtatt
     3541 cctctgggag atcctctgca gcctacccgt gtaaaagatc ctgagacacg tgagattatc
     3601 ctgatggacc tggttcgtcc tcaagacacc tatcagtttt tcgtcaaaca cggcgagctg
     3661 aaagtctata aaaccctgga caccccgttc tttagtaccg gcaaactgat tctgggtcct
     3721 caggaggaaa aaggcaaaca gcacgtgggt caagacatcc tggtctttta tgtgaatttt
     3781 ggggacctgc tgtgtactct gcatgaaacc ccgtatattc tgtctaccgg tgacagcttc
     3841 tatgtgccgt ctggaaacta ttataacatc aaaaacctgc gcaacgaaga aagtgttctg
     3901 ctgttcacac agatcaaacg ctgagtcgac gagctcactt gtcgcggccg ctttcgaatc
     3961 tagagcctgc agtctcgaca agcttgtcga gaagtactag aggatcataa tcagccatac
     4021 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     4081 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     4141 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     4201 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctgatcac tgcttgagcc
     4261 tagaagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
     4321 agcaataact atcataaccc ctagggtata cccatctaat tggaaccaga taagtgaaat
     4381 ctagttccaa actattttgt catttttaat tttcgtatta gcttacgacg ctacacccag
     4441 ttcccatcta ttttgtcact cttccctaaa taatccttaa aaactccatt tccacccctc
     4501 ccagttccca actattttgt ccgcccacaa ccggttgact tgggtcaact gtcagaccaa
     4561 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     4621 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     4681 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     4741 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     4801 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     4861 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     4921 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     4981 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     5041 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     5101 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     5161 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     5221 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     5281 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     5341 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttgacttggg
     5401 tcgctcttcc tgtggatgcg cagatgccct gcgtaagcgg gtgtgggcgg acaataaagt
     5461 cttaaactga acaaaataga tctaaactat gacaataaag tcttaaacta gacagaatag
     5521 ttgtaaactg aaatcagtcc agttatgctg tgaaaaagca tactggactt ttgttatggc
     5581 taaagcaaac tcttcatttt ctgaagtgca aattgcccgt cgtattaaag aggggcgtgg
     5641 ccaagggcat gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg
     5701 ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa
     5761 agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac
     5821 cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga
     5881 gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat
     5941 agatatagat ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc
     6001 caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt
     6061 tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac
     6121 gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg
     6181 tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg
     6241 taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg
     6301 gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa
     6361 caagccatga aaaccgccac tgcgccgtta ccaccgc
//
