LOCUS       sequence-272319-         639 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to sense miRNA activity using fluorescence based tools. 
            hsa-miR-3074-5p target in 3' UTR of of mKate. miRNA activity can be 
            measured by the repression of mKate2 relative to EBFP2 fluorescent 
            proteins . Plasmid is inherently unstable; please see Depositor 
            Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB-hsa-miR-3074-5p
FEATURES             Location/Qualifiers
     source          1..639
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(109..133)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(353..372)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    418..473
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(472..491)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 tcaaggaggc cgacaaagag acatacgtcg agcagcacga ggtggctgtg gccagatact
       61 gcgacctccc tagcaaactg gggcacaaac ttaattgaga attctaccac ccagctttct
      121 tgtacaaagt ggtacgtctc aagaccagag gtctctgctt ggctcgatct ggctcagttc
      181 agcaggaacc tggctcagtt cagcaggaac ctggctcagt tcagcaggaa cctggctcag
      241 ttcagcagga accaaccaga agagacctgt gcttcactcc tcaggtgcag gctgcctatc
      301 agaaggtggt ggctggtgtg gccaatgccc tggctcacaa ataccactga gatctttttc
      361 cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact tctggctaat
      421 aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa
      481 ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg
      541 caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt
      601 atatgaaaca gccccctgct gtccattcct tattccata
//
