LOCUS       sequence-272329-        5949 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For incorporation of fluorophenylalanine derivatives.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5949)
  AUTHORS   Lee YJ, Schmidt MJ, Tharp JM, Weber A, Koenig AL, Zheng H, Gao J, 
            Waters ML, Summerer D, Liu WR
  TITLE     Genetically encoded fluorophenylalanines enable insights into the 
            recognition of lysine trimethylation by an epigenetic reader.
  JOURNAL   Chem Commun (Camb). 2016 Oct 18;52(85):12606-12609. doi: 
            10.1039/c6cc05959g.
  PUBMED    27711380
REFERENCE   2  (bases 1 to 5949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Chem Commun (Camb)"; date: "2016-10-18- 18"; volume: "52"; issue: 
            "85"; pages: "12606-12609"
COMMENT     Sequence Label: PylRS-AS
FEATURES             Location/Qualifiers
     source          1..5949
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     208..227
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             1693..1710
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1766..1783)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     CDS             complement(3066..3725)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3640..3659
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(3726..3828)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(4220..4237)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4354..4899)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(4434..4453)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5045..5923)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPSDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg
      301 ctaacaggag gaattactag tatggataaa aaaccactaa acactctgat atctgcaacc
      361 gggctctgga tgtccaggac cggaacaatt cataaaataa aacaccacga agtctctcga
      421 agcaaaatct atattgaaat ggcatgcggt gaccaccttg ttgtaaacaa ctccaggagc
      481 agcaggactg caagagcgct caggcaccac aaatacagga agacctgcaa acgctgcagg
      541 gtttcggatg aggatctcaa taagttcctc acaaaggcaa acgaagacca gacaagcgta
      601 aaagtcaagg tcgtttctgc ccctaccaga acgaaaaagg caatgccaaa atccgttgcg
      661 agagccccga aacctcttga gaatacagaa gcggcacagg ctcaaccttc tggatctaaa
      721 ttttcacctg cgataccggt ttccacccaa gagtcagttt ctgtcccggc atctgtttca
      781 acatcaatat caagcatttc tacaggagca actgcatccg cactggtaaa agggaatacg
      841 aaccccatta catccatgtc tgcccctgtt caggcaagtg cccccgcact tacgaagagc
      901 cagactgaca ggcttgaagt cctgttaaac ccaaaagatg agatttccct gaattccggc
      961 aagcctttca gggagcttga gtccgaattg ctctctcgca gaaaaaaaga cctgcagcag
     1021 atctacgcgg aagaaaggga gaattatctg gggaaactcg agcgtgaaat taccaggttc
     1081 tttgtggaca ggggttttct ggaaataaaa tccccgatcc tgatccctct tgagtatatc
     1141 gaaaggatgg gcattgataa tgataccgaa ctttcaaaac agatcttcag ggttgacaag
     1201 aacttctgcc tgagacccat gcttgctcca aacctttaca actacctgcg caagcttgac
     1261 agggccctgc ctgatccaat aaaaattttt gaaataggcc catgctacag aaaagagtcc
     1321 gacggcaaag aacacctcga agagtttacc atgctggcgt tctctcagat gggatcggga
     1381 tgcacacggg aaaatcttga aagcataatt acggacttcc tgaaccacct gggaattgat
     1441 ttcaagatcg taggcgattc ctgcatggtc tatggggata cccttgatgt aatgcacgga
     1501 gacctggaac tttcctctgc agtagtcgga cccataccgc ttgaccggga atggggtatt
     1561 gataaaccct ggataggggc aggtttcggg ctcgaacgcc ttctaaaggt taaacacgac
     1621 tttaaaaata tcaagagagc tgcaaggtcc gagtcttact ataacgggat ttctaccaac
     1681 ctgtaagtcg accatcatca tcatcatcat tgagtttaaa cggtctccag cttggctgtt
     1741 ttggcggatg agagaagatt ttcagcctga tacagattaa atcagaacgc agaagcggtc
     1801 tgataaaaca gaatttgcct ggcggcagta gcgcggtggt cccacctgac cccatgccga
     1861 actcagaagt gaaacgccgt agcgccgatg gtagtgtggg gtctccccat gcgagagtag
     1921 ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc cttgtttgtg
     1981 agctcccggt catcaatcat ccccataatc cttgttagat tatcaatttt aaaaaactaa
     2041 cagttgtcag cctgtcccgc tttaatatca tacgccgtta tacgttgttt acgctttgag
     2101 gaatcccata tgatacgcgg ccgctttcaa acgctaaatt gcctgatgcg ctacgcttat
     2161 caggcctaca tgatctctgc aatatattga gtttgcgtgc ttttgtaggc cggataaggc
     2221 gttcacgccg catccggcaa gaaacagcaa acaatccaaa acgccgcgtt cagcggcgtt
     2281 ttttctgctt ttcttcgcga attaattccg cttcgcaaca tgtgagcacc ggtttattga
     2341 ctaccggaag cagtgtgacc gtgtgcttct caaatgcctg aggccagttt gctcaggctc
     2401 tccccgtgga ggtaataatt gacgatatga tcagtgcacg gctaactaag cggcctgctg
     2461 actttctcgc cgatcaaaag gcattttgct attaagggat tgacgagggc gtatctgcgc
     2521 agtaagatgc gccccgcatt ggaaacctga tcatgtagat cgaatggact ctaaatccgt
     2581 tcagccgggt tagattcccg gggtttcctc caaattcgaa aagcctgctc aacgagcagg
     2641 cttttttgca tgctcgactg tgcttctcaa atgcctgagg ccagtttgct caggctctcc
     2701 ccgtggaggt aataattgac gatatgatca gtgcacggct aactaagcgg cctgctgact
     2761 ttctcgccga tcaaaaggca ttttgctatt aagggattga cgagggcgta tctgcgcagt
     2821 aagatgcgcc ccgcattgga aacctgatca tgtagatcga atggactcta aatccgttca
     2881 gccgggttag attcccgggg tttcctccaa attcgaaaag cctgctcaac gagcaggctt
     2941 ttttgcatgc tcgagcagct cagggtcgaa tttgctttcg aatttctgcc attcatccgc
     3001 ttattatcac ttattcaggc gtagcaacca ggcgtttaag ggcaccaata actgccttaa
     3061 aaaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
     3121 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
     3181 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
     3241 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac
     3301 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
     3361 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
     3421 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
     3481 agctcaccgt ctttcattgc catacggaat tccggatgag cattcatcag gcgggcaaga
     3541 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
     3601 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
     3661 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
     3721 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
     3781 gatcttattt cattatggtg aaagttggaa cctcttacgt gccgatcaac gtctcatttt
     3841 cgccaaaagt tggcccaggg cttcccggta tcaacaggga caccaggatt tatttattct
     3901 gcgaagtgat cttccgtcac aggtatttat tcggcgcaaa gtgcgtcggg tgatgctgcc
     3961 aacttactga tttagtgtat gatggtgttt ttgaggtgct ccagtggctt ctgtttctat
     4021 cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt aacggcaaaa gcaccgccgg
     4081 acatcagcgc tagcggagtg tatactggct tactatgttg gcactgatga gggtgtcagt
     4141 gaagtgcttc atgtggcagg agaaaaaagg ctgcaccggt gcgtcagcag aatatgtgat
     4201 acaggatata ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cgactgcggc
     4261 gagcggaaat ggcttacgaa cggggcggag atttcctgga agatgccagg aagatactta
     4321 acagggaagt gagagggccg cggcaaagcc gtttttccat aggctccgcc cccctgacaa
     4381 gcatcacgaa atctgacgct caaatcagtg gtggcgaaac ccgacaggac tataaagata
     4441 ccaggcgttt ccccctggcg gctccctcgt gcgctctcct gttcctgcct ttcggtttac
     4501 cggtgtcatt ccgctgttat ggccgcgttt gtctcattcc acgcctgaca ctcagttccg
     4561 ggtaggcagt tcgctccaag ctggactgta tgcacgaacc ccccgttcag tccgaccgct
     4621 gcgccttatc cggtaactat cgtcttgagt ccaacccgga aagacatgca aaagcaccac
     4681 tggcagcagc cactggtaat tgatttagag gagttagtct tgaagtcatg cgccggttaa
     4741 ggctaaactg aaaggacaag ttttggtgac tgcgctcctc caagccagtt acctcggttc
     4801 aaagagttgg tagctcagag aaccttcgaa aaaccgccct gcaaggcggt tttttcgttt
     4861 tcagagcaag agattacgcg cagaccaaaa cgatctcaag aagatcatct tattaatcag
     4921 ataaaatatt tctagatttc agtgcaattt atctcttcaa atgtagcacc tgaagtcagc
     4981 cccatacgat ataagttgta attctcatgt ttgacagctt atcatcgata agcttggtac
     5041 ccaattatga caacttgacg gctacatcat tcactttttc ttcacaaccg gcacggaact
     5101 cgctcgggct ggccccggtg cattttttaa atacccgcga gaaatagagt tgatcgtcaa
     5161 aaccaacatt gcgaccgacg gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg
     5221 cctggctgat acgttggtcc tcgcgccagc ttaagacgct aatccctaac tgctggcgga
     5281 aaagatgtga cagacgcgac ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa
     5341 aattgctgtc tgccaggtga tcgctgatgt actgacaagc ctcgcgtacc cgattatcca
     5401 tcggtggatg gagcgactcg ttaatcgctt ccatgcgccg cagtaacaat tgctcaagca
     5461 gatttatcgc cagcagctcc gaatagcgcc cttccccttg cccggcgtta atgatttgcc
     5521 caaacaggtc gctgaaatgc ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg
     5581 caaatattga cggccagtta agccattcat gccagtaggc gcgcggacga aagtaaaccc
     5641 actggtgata ccattcgcga gcctccggat gacgaccgta gtgatgaatc tctcctggcg
     5701 ggaacagcaa aatatcactc ggtcggcaaa caaattctcg tccctgattt ttcaccaccc
     5761 cctgaccgcg aatggtgaga ttgagaatat aacctttcat tcccagcggt cggtcgataa
     5821 aaaaatcgag ataaccgttg gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa
     5881 acgagtatcc cggcagcagg ggatcatttt gcgcttcagc catacttttc atactcccgc
     5941 cattcagag
//
