LOCUS       sequence-27234-e         667 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Furukawa M, Xiong Y
  TITLE     BTB protein Keap1 targets antioxidant transcription factor Nrf2 for 
            ubiquitination by the Cullin 3-Roc1 ligase.
  JOURNAL   Mol Cell Biol. 2005 Jan . 25(1):162-71.
  PUBMED    15601839
REFERENCE   2  (bases 1 to 667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2005 Jan . 25(1):162-71."
COMMENT     Sequence Label: pPROEX-HTc-Flag3-Nrf2
FEATURES             Location/Qualifiers
     source          1..667
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2..19
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             41..61
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             71..136
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ccatcaccat caccatcacg attacgatat cccaacgacc gaaaacctgt attttcaggg
       61 cgccatgatg gactacaaag accatgacgg tgattataaa gatcatgata tcgattacaa
      121 ggatgacgat gacaaggcca tggggatcct catgatggac ttggagctgc cgccgccggg
      181 actcccgtcc cagcaggaca tggatttgat tgacatactt tggaggcaag atatagatct
      241 tggagtaagt cgagaagtat ttgacttcag tcagcgacgg aaagagtatg agctggaaaa
      301 acagaaaaaa cttgaaaagg aaagacaaga acaactccaa aaggagcaag agaaagcctt
      361 tttcgctcag ttacaactag atgaagagac aggtgaattt ctcccaattc agccagccca
      421 gcacatccag tcagaaacca gtggatctgc caactactcc caggttgccc acattcccaa
      481 atcagatgct ttgtactttg atgactgcat gcagcttttg gcgcagacat tcccgtttgt
      541 agatgacaat gaggtttctt cggctacgtt tcagtcactt gttcctgata ttcccggtca
      601 catcgagagc ccagtcttca ttgctactaa tcaggctcag tcacctgaaa cttctgttgc
      661 tcaggta
//
