LOCUS       sequence-272342-        4398 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive mammalian overexpression vector for human bone 
            morphogenetic protein 7 cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Feichtinger GA, Hacobian A, Hofmann AT, Wassermann K, Zimmermann A, 
            van Griensven M, Redl H
  TITLE     Constitutive and inducible co-expression systems for non-viral 
            osteoinductive gene therapy.
  JOURNAL   Eur Cell Mater. 2014 Feb 19;27:166-84; discussion 184.
  PUBMED    24554273
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eur Cell 
            Mater."; date: "2014-02-19"; volume: "27"; pages: "166-84; 
            discussion 184"
COMMENT     Sequence Label: pVAX1-BMP7
FEATURES             Location/Qualifiers
     source          1..4398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        109..488
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        489..692
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     643..663
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     737..756
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        737..755
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2296..2313)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2302..2526
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             2699..3493
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2699..3493
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2753..2772)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3363..3382
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      join(3818..4398,1..8)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4307..4326
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
       61 ctcacatgtt cttgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat
      121 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
      181 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      241 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      301 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      361 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      421 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      481 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      541 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      601 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      661 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactagagaa cccactgctt
      721 actggcttat cgaaattaat acgactcact atagggagac ccaagctggc tagcgtttaa
      781 acttaagctt gccaccgcga tgcacgtgcg ctcactgcga gctgcggcgc cgcacagctt
      841 cgtggcgctc tgggcacccc tgttcctgct gcgctccgcc ctggccgact tcagcctgga
      901 caacgaggtg cactcgagct tcatccaccg gcgcctccgc agccaggagc ggcgggagat
      961 gcagcgcgag atcctctcca ttttgggctt gccccaccgc ccgcgcccgc acctccaggg
     1021 caagcacaac tcggcaccca tgttcatgct ggacctgtac aacgccatgg cggtggagga
     1081 gggcggcggg cccggcggcc agggcttctc ctacccctac aaggccgtct tcagtaccca
     1141 gggcccccct ctggccagcc tgcaagatag ccatttcctc accgacgccg acatggtcat
     1201 gagcttcgtc aacctcgtgg aacatgacaa ggaattcttc cacccacgct accaccatcg
     1261 agagttccgg tttgatcttt ccaagatccc agaaggggaa gctgtcacgg cagccgaatt
     1321 ccggatctac aaggactaca tccgggaacg cttcgacaat gagacgttcc ggatcagcgt
     1381 ttatcaggtg ctccaggagc acttgggcag ggaatcggat ctcttcctgc tcgacagccg
     1441 taccctctgg gcctcggagg agggctggct ggtgtttgac atcacagcca ccagcaacca
     1501 ctgggtggtc aatccgcggc acaacctggg cctgcagctc tcggtggaga cgctggatgg
     1561 gcagagcatc aaccccaagt tggcgggcct gattgggcgg cacgggcccc agaacaagca
     1621 gcccttcatg gtggctttct tcaaggccac ggaggtccac ttccgcagca tccggtccac
     1681 ggggagcaaa cagcgcagcc agaaccgctc caagacgccc aagaaccagg aagccctgcg
     1741 gatggccaac gtggcagaga acagcagcag cgaccagagg caggcctgta agaagcacga
     1801 gctgtatgtc agcttccgag acctgggctg gcaggactgg atcatcgcgc ctgaaggcta
     1861 cgccgcctac tactgtgagg gggagtgtgc cttccctctg aactcctaca tgaacgccac
     1921 caaccacgcc atcgtgcaga cgctggtcca cttcatcaac ccggaaacgg tgcccaagcc
     1981 ctgctgtgcg cccacgcagc tcaatgccat ctccgtcctc tacttcgatg acagctccaa
     2041 cgtcatcctg aagaaataca gaaacatggt ggtccgggcc tgtggctgcc actagctcct
     2101 ccgagaattc agaccctttg gggccaagtt tttctggatc ctccattgct cgccttggcc
     2161 aggaaccagc agaccaactg ccttttgtga gaccttcccc tccctatccc caactttaaa
     2221 ggtgtgagag tattaggaaa catgagcagc agcggccgct cgagtctaga gggcccgttt
     2281 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     2341 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     2401 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     2461 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     2521 ctatggcttc tactgggcgg ttttatggac agcaagcgaa ccggaattgc cagctggggc
     2581 gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct tgccgccaag
     2641 gatctgatgg cgcaggggat caagctctga tcaagagaca ggatgaggat cgtttcgcat
     2701 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     2761 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     2821 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     2881 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     2941 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     3001 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     3061 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     3121 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     3181 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg
     3241 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     3301 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     3361 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     3421 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     3481 cgagttcttc tgaattatta acgcttacaa tttcctgatg cggtattttc tccttacgca
     3541 tctgtgcggt atttcacacc gcatcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     3601 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     3661 gataaatgct tcaataatag cacgtgctaa aacttcattt ttaatttaaa aggatctagg
     3721 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     3781 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3841 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3901 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3961 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     4021 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     4081 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     4141 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     4201 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     4261 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     4321 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     4381 cgtcaggggg gcggagcc
//
