LOCUS       sequence-272346-        3218 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive mammalian co-expression vector for human bone 
            morphogenetic protein 2 and 7 cDNAs (2 transcriptional units, 
            tandem).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Feichtinger GA, Hacobian A, Hofmann AT, Wassermann K, Zimmermann A, 
            van Griensven M, Redl H
  TITLE     Constitutive and inducible co-expression systems for non-viral 
            osteoinductive gene therapy.
  JOURNAL   Eur Cell Mater. 2014 Feb 19;27:166-84; discussion 184.
  PUBMED    24554273
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eur Cell 
            Mater."; date: "2014-02-19"; volume: "27"; pages: "166-84; 
            discussion 184"
COMMENT     Sequence Label: pVAX1-BMP2/7 +
FEATURES             Location/Qualifiers
     source          1..3218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     249..268
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        249..267
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1808..1825)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1814..2038
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2150..2738
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2639..2658
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        2839..3218
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc ttactggctt
      241 atcgaaatta atacgactca ctatagggag acccaagctg gctagcgttt aaacttaagc
      301 ttgccaccgc gatgcacgtg cgctcactgc gagctgcggc gccgcacagc ttcgtggcgc
      361 tctgggcacc cctgttcctg ctgcgctccg ccctggccga cttcagcctg gacaacgagg
      421 tgcactcgag cttcatccac cggcgcctcc gcagccagga gcggcgggag atgcagcgcg
      481 agatcctctc cattttgggc ttgccccacc gcccgcgccc gcacctccag ggcaagcaca
      541 actcggcacc catgttcatg ctggacctgt acaacgccat ggcggtggag gagggcggcg
      601 ggcccggcgg ccagggcttc tcctacccct acaaggccgt cttcagtacc cagggccccc
      661 ctctggccag cctgcaagat agccatttcc tcaccgacgc cgacatggtc atgagcttcg
      721 tcaacctcgt ggaacatgac aaggaattct tccacccacg ctaccaccat cgagagttcc
      781 ggtttgatct ttccaagatc ccagaagggg aagctgtcac ggcagccgaa ttccggatct
      841 acaaggacta catccgggaa cgcttcgaca atgagacgtt ccggatcagc gtttatcagg
      901 tgctccagga gcacttgggc agggaatcgg atctcttcct gctcgacagc cgtaccctct
      961 gggcctcgga ggagggctgg ctggtgtttg acatcacagc caccagcaac cactgggtgg
     1021 tcaatccgcg gcacaacctg ggcctgcagc tctcggtgga gacgctggat gggcagagca
     1081 tcaaccccaa gttggcgggc ctgattgggc ggcacgggcc ccagaacaag cagcccttca
     1141 tggtggcttt cttcaaggcc acggaggtcc acttccgcag catccggtcc acggggagca
     1201 aacagcgcag ccagaaccgc tccaagacgc ccaagaacca ggaagccctg cggatggcca
     1261 acgtggcaga gaacagcagc agcgaccaga ggcaggcctg taagaagcac gagctgtatg
     1321 tcagcttccg agacctgggc tggcaggact ggatcatcgc gcctgaaggc tacgccgcct
     1381 actactgtga gggggagtgt gccttccctc tgaactccta catgaacgcc accaaccacg
     1441 ccatcgtgca gacgctggtc cacttcatca acccggaaac ggtgcccaag ccctgctgtg
     1501 cgcccacgca gctcaatgcc atctccgtcc tctacttcga tgacagctcc aacgtcatcc
     1561 tgaagaaata cagaaacatg gtggtccggg cctgtggctg ccactagctc ctccgagaat
     1621 tcagaccctt tggggccaag tttttctgga tcctccattg ctcgccttgg ccaggaacca
     1681 gcagaccaac tgccttttgt gagaccttcc cctccctatc cccaacttta aaggtgtgag
     1741 agtattagga aacatgagca gcagcggccg ctcgagtcta gagggcccgt ttaaacccgc
     1801 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     1861 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     1921 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     1981 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     2041 tctactgggc ggttttatgg acagcaagcg aacacatgac caaaatccct taacgtgagt
     2101 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     2161 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     2221 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     2281 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     2341 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     2401 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     2461 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     2521 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     2581 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     2641 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     2701 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     2761 tacggttcct ggccttttgc tggccttttg ctcacatgtt cttgctgctt cgcgatgtac
     2821 gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
     2881 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     2941 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     3001 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     3061 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     3121 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     3181 ggcagtacat ctacgtatta gtcatcgcta ttaccatg
//
