LOCUS       sequence-272349-        6597 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive mammalian co-expression vector for human bone 
            morphogenetic protein 2 and 7 cDNAs (2 transcriptional units, 
            divergent).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6597)
  AUTHORS   Feichtinger GA, Hacobian A, Hofmann AT, Wassermann K, Zimmermann A, 
            van Griensven M, Redl H
  TITLE     Constitutive and inducible co-expression systems for non-viral 
            osteoinductive gene therapy.
  JOURNAL   Eur Cell Mater. 2014 Feb 19;27:166-84; discussion 184.
  PUBMED    24554273
REFERENCE   2  (bases 1 to 6597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eur Cell 
            Mater."; date: "2014-02-19"; volume: "27"; pages: "166-84; 
            discussion 184"
COMMENT     Sequence Label: pVAX1-BMP2/7 -
FEATURES             Location/Qualifiers
     source          1..6597
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        158..537
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      640..1228
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1129..1148
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        1329..1708
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1709..1912
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        1957..1975
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2057..2066
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2061..2070
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3335..3559
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             3732..4526
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3732..4526
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3786..3805)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4396..4415
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4717..4941
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        complement(6488..6506)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(join(6551..6597,1..157))
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa
       61 acaaactccc attgacgtca atggggtgga gacttggaaa tccccgtgag tcaaaccgct
      121 atccacgccc attgatgtac tgccaaaacc gcatcaccat ggtaatagcg atgactaata
      181 cgtagatgta ctgccaagta ggaaagtccc ataaggtcat gtactgggca taatgccagg
      241 cgggccattt accgtcattg acgtcaatag ggggcgtact tggcatatga tacacttgat
      301 gtactgccaa gtgggcagtt taccgtaaat actccaccca ttgacgtcaa tggaaagtcc
      361 ctattggcgt tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg
      421 gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac gcggaactcc atatatgggc
      481 tatgaactaa tgaccccgta attgattact attaataact agtcaataat caatgtcaac
      541 gcgtatatct ggcccgtaca tcgacatgac caaaatccct taacgtgagt tttcgttcca
      601 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
      661 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
      721 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
      781 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
      841 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
      901 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
      961 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1021 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1081 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1141 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1201 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     1261 ggccttttgc tggccttttg ctcacatgtt cttgctgctt cgcgatgtac gggccagata
     1321 tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
     1381 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     1441 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     1501 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     1561 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     1621 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     1681 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
     1741 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     1801 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
     1861 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctctggct
     1921 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
     1981 ccaagctggc tagcgtttaa acttaagctt ggtaccgagc tcggatccac tagtccagtg
     2041 tggtggaatt cggcttgcca ccatggtggc cgggacccgc tgtcttctag cgttgctgct
     2101 tccccaggtc ctcctgggcg gcgcggctgg cctcgttccg gagctgggcc gcaggaagtt
     2161 cgcggcggcg tcgtcgggcc gcccctcatc ccagccctct gacgaggtcc tgagcgagtt
     2221 cgagctgcgg ctgctcagca tgttcggcct gaaacagaga cccaccccca gcagggacgc
     2281 cgtggtgccc ccctacatgc tagacctgta tcgcaggcac tcaggtcagc cgggctcacc
     2341 cgccccagac caccggttgg agagggcagc cagccgagcc aacactgtgc gcagcttcca
     2401 ccatgaagaa tctttggaag aactaccaga aacgagtggg aaaacaaccc ggagattctt
     2461 ctttaattta agttctatcc ccacggagga gtttatcacc tcagcagagc ttcaggtttt
     2521 ccgagaacag atgcaagatg ctttaggaaa caatagcagt ttccatcacc gaattaatat
     2581 ttatgaaatc ataaaacctg caacagccaa ctcgaaattc cccgtgacca gacttttgga
     2641 caccaggttg gtgaatcaga atgcaagcag gtgggaaagt cttgatgtca cccccgctgt
     2701 gatgcggtgg actgcacagg gacacgccaa ccatggattc gtggtggaag tggcccactt
     2761 ggaggagaaa caaggtgtct ccaagagaca tgttaggata agcaggtctt tgcaccaaga
     2821 tgaacacagc tggtcacaga taaggccatt gctagtaact tttggccatg atggaaaagg
     2881 gcatcctctc cacaaaagag aaaaacgtca agccaaacac aaacagcgga aacgccttaa
     2941 gtccagctgt aagagacacc ctttgtacgt ggacttcagt gacgtggggt ggaatgactg
     3001 gattgtggct cccccggggt atcacgcctt ttactgccac ggagaatgcc cttttcctct
     3061 ggctgatcat ctgaactcca ctaatcatgc cattgttcag acgttggtca actctgttaa
     3121 ctctaagatt cctaaggcat gctgtgtccc gacagaactc agtgctatct cgatgctgta
     3181 ccttgacgag aatgaaaagg ttgtattaaa gaactatcag gacatggttg tggagggttg
     3241 tgggtgtcgc tagaagccga attctgcaga tatccagcac agtggcggcc gctcgagtct
     3301 agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     3361 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     3421 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     3481 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     3541 gatgcggtgg gctctatggc ttctactggg cggttttatg gacagcaagc gaaccggaat
     3601 tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt
     3661 tcttgccgcc aaggatctga tggcgcaggg gatcaagctc tgatcaagag acaggatgag
     3721 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     3781 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     3841 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     3901 tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     3961 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     4021 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     4081 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     4141 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     4201 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga
     4261 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     4321 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     4381 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     4441 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     4501 atcgccttct tgacgagttc ttctgaatta ttaacgctta caatttcctg atgcggtatt
     4561 ttctccttac gcatctgtgc ggtatttcac accgcatcag gtggcacttt tcggggaaat
     4621 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     4681 tgttcgcttg ctgtccataa aaccgcccag tagaagccat agagcccacc gcatccccag
     4741 catgcctgct attgtcttcc caatcctccc ccttgctgtc ctgccccacc ccacccccca
     4801 gaatagaatg acacctactc agacaatgcg atgcaatttc ctcattttat taggaaagga
     4861 cagtgggagt ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa acaacagatg
     4921 gctggcaact agaaggcaca gtcgaggctg atcagcgggt ttaaacgggc cctctagact
     4981 cgagcggccg ctgctgctca tgtttcctaa tactctcaca cctttaaagt tggggatagg
     5041 gaggggaagg tctcacaaaa ggcagttggt ctgctggttc ctggccaagg cgagcaatgg
     5101 aggatccaga aaaacttggc cccaaagggt ctgaattctc ggaggagcta gtggcagcca
     5161 caggcccgga ccaccatgtt tctgtatttc ttcaggatga cgttggagct gtcatcgaag
     5221 tagaggacgg agatggcatt gagctgcgtg ggcgcacagc agggcttggg caccgtttcc
     5281 gggttgatga agtggaccag cgtctgcacg atggcgtggt tggtggcgtt catgtaggag
     5341 ttcagaggga aggcacactc cccctcacag tagtaggcgg cgtagccttc aggcgcgatg
     5401 atccagtcct gccagcccag gtctcggaag ctgacataca gctcgtgctt cttacaggcc
     5461 tgcctctggt cgctgctgct gttctctgcc acgttggcca tccgcagggc ttcctggttc
     5521 ttgggcgtct tggagcggtt ctggctgcgc tgtttgctcc ccgtggaccg gatgctgcgg
     5581 aagtggacct ccgtggcctt gaagaaagcc accatgaagg gctgcttgtt ctggggcccg
     5641 tgccgcccaa tcaggcccgc caacttgggg ttgatgctct gcccatccag cgtctccacc
     5701 gagagctgca ggcccaggtt gtgccgcgga ttgaccaccc agtggttgct ggtggctgtg
     5761 atgtcaaaca ccagccagcc ctcctccgag gcccagaggg tacggctgtc gagcaggaag
     5821 agatccgatt ccctgcccaa gtgctcctgg agcacctgat aaacgctgat ccggaacgtc
     5881 tcattgtcga agcgttcccg gatgtagtcc ttgtagatcc ggaattcggc tgccgtgaca
     5941 gcttcccctt ctgggatctt ggaaagatca aaccggaact ctcgatggtg gtagcgtggg
     6001 tggaagaatt ccttgtcatg ttccacgagg ttgacgaagc tcatgaccat gtcggcgtcg
     6061 gtgaggaaat ggctatcttg caggctggcc agaggggggc cctgggtact gaagacggcc
     6121 ttgtaggggt aggagaagcc ctggccgccg ggcccgccgc cctcctccac cgccatggcg
     6181 ttgtacaggt ccagcatgaa catgggtgcc gagttgtgct tgccctggag gtgcgggcgc
     6241 gggcggtggg gcaagcccaa aatggagagg atctcgcgct gcatctcccg ccgctcctgg
     6301 ctgcggaggc gccggtggat gaagctcgag tgcacctcgt tgtccaggct gaagtcggcc
     6361 agggcggagc gcagcaggaa caggggtgcc cagagcgcca cgaagctgtg cggcgccgca
     6421 gctcgcagtg agcgcacgtg catcgcggtg gcaagcttaa gtttaaacgc tagccagctt
     6481 gggtctccct atagtgagtc gtattaattt cgataagcca gtaagcagtg ggttctctag
     6541 ttagccagag agctctgctt atatagacct cccaccgtac acgcctaccg cccattt
//
