LOCUS       sequence-272351-        4211 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive mammalian overexpression vector for human bone 
            morphogenetic protein 2 cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Feichtinger GA, Hacobian A, Hofmann AT, Wassermann K, Zimmermann A, 
            van Griensven M, Redl H
  TITLE     Constitutive and inducible co-expression systems for non-viral 
            osteoinductive gene therapy.
  JOURNAL   Eur Cell Mater. 2014 Feb 19;27:166-84; discussion 184.
  PUBMED    24554273
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eur Cell 
            Mater."; date: "2014-02-19"; volume: "27"; pages: "166-84; 
            discussion 184"
COMMENT     Sequence Label: pVAX1-BMP2
FEATURES             Location/Qualifiers
     source          1..4211
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     249..268
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        249..267
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      349..358
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      353..362
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1621..1638)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1627..1851
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             2024..2818
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2024..2818
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2078..2097)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2688..2707
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      3143..3731
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3632..3651
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        3832..4211
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc ttactggctt
      241 atcgaaatta atacgactca ctatagggag acccaagctg gctagcgttt aaacttaagc
      301 ttggtaccga gctcggatcc actagtccag tgtggtggaa ttcggcttgc caccatggtg
      361 gccgggaccc gctgtcttct agcgttgctg cttccccagg tcctcctggg cggcgcggct
      421 ggcctcgttc cggagctggg ccgcaggaag ttcgcggcgg cgtcgtcggg ccgcccctca
      481 tcccagccct ctgacgaggt cctgagcgag ttcgagctgc ggctgctcag catgttcggc
      541 ctgaaacaga gacccacccc cagcagggac gccgtggtgc ccccctacat gctagacctg
      601 tatcgcaggc actcaggtca gccgggctca cccgccccag accaccggtt ggagagggca
      661 gccagccgag ccaacactgt gcgcagcttc caccatgaag aatctttgga agaactacca
      721 gaaacgagtg ggaaaacaac ccggagattc ttctttaatt taagttctat ccccacggag
      781 gagtttatca cctcagcaga gcttcaggtt ttccgagaac agatgcaaga tgctttagga
      841 aacaatagca gtttccatca ccgaattaat atttatgaaa tcataaaacc tgcaacagcc
      901 aactcgaaat tccccgtgac cagacttttg gacaccaggt tggtgaatca gaatgcaagc
      961 aggtgggaaa gtcttgatgt cacccccgct gtgatgcggt ggactgcaca gggacacgcc
     1021 aaccatggat tcgtggtgga agtggcccac ttggaggaga aacaaggtgt ctccaagaga
     1081 catgttagga taagcaggtc tttgcaccaa gatgaacaca gctggtcaca gataaggcca
     1141 ttgctagtaa cttttggcca tgatggaaaa gggcatcctc tccacaaaag agaaaaacgt
     1201 caagccaaac acaaacagcg gaaacgcctt aagtccagct gtaagagaca ccctttgtac
     1261 gtggacttca gtgacgtggg gtggaatgac tggattgtgg ctcccccggg gtatcacgcc
     1321 ttttactgcc acggagaatg cccttttcct ctggctgatc atctgaactc cactaatcat
     1381 gccattgttc agacgttggt caactctgtt aactctaaga ttcctaaggc atgctgtgtc
     1441 ccgacagaac tcagtgctat ctcgatgctg taccttgacg agaatgaaaa ggttgtatta
     1501 aagaactatc aggacatggt tgtggagggt tgtgggtgtc gctagaagcc gaattctgca
     1561 gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag
     1621 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     1681 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     1741 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     1801 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctactg
     1861 ggcggtttta tggacagcaa gcgaaccgga attgccagct ggggcgccct ctggtaaggt
     1921 tgggaagccc tgcaaagtaa actggatggc tttcttgccg ccaaggatct gatggcgcag
     1981 gggatcaagc tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     2041 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     2101 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     2161 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg
     2221 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     2281 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     2341 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     2401 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     2461 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     2521 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt
     2581 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     2641 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     2701 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     2761 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgaat
     2821 tattaacgct tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     2881 acaccgcatc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     2941 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     3001 aatagcacgt gctaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     3061 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     3121 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     3181 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     3241 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     3301 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     3361 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     3421 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     3481 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     3541 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     3601 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     3661 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     3721 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     3781 ttgctcacat gttcttgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat
     3841 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     3901 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     3961 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     4021 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     4081 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     4141 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     4201 ctattaccat g
//
