LOCUS       sequence-272356-       11184 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses APOB-Linker-eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11184)
  AUTHORS   Walsh MT, Celestin OM, Thierer JH, Rajan S, Farber SA, Hussain MM
  TITLE     Model systems to study assembly, trafficking and secretion of 
            apoB-lipoproteins using fluorescent fusion proteins.
  JOURNAL   J Lipid Res. 2019 Dec 30. pii: jlr.RA119000259. doi: 
            10.1194/jlr.RA119000259.
  PUBMED    31888978
REFERENCE   2  (bases 1 to 11184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Lipid 
            Res. 2019 Dec 30. pii: jlr.RA119000259. doi: 
            10.1194/jlr.RA119000259."
COMMENT     Sequence Label: pRP.ExTri-CMV-5'UTR-ApoB48-Linker-eGFP
FEATURES             Location/Qualifiers
     source          1..11184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    225..249
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(6961..6963,6964..6966,6967..7680)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6961..6963,6964..6966,6967..7677)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7006..7027)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7267..7286)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7614..7635
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(7681..7705)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    7734..7855
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7745..7764)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7799..7818
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8103..8120)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8274..8862)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8354..8373)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9033..9824,9825..9893))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9656..9675
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9894..9998)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      10025..10480
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10112..10131)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10322..10343
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    10611..10659
                     /note="synthetic polyadenylation signal"
     misc_feature    10673..10764
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     10713..10732
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     protein_bind    10800..10820
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        10881..11184
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccaagt ttgtacaaaa
      241 aagcaggctg gaattccata tgttcgaaac tgcaggaatt cccaccggga cctgcggggc
      301 tgagtgccct tctcggttgc tgccgctgag gagcccgccc agccagccag ggccgcgagg
      361 ccgaggccag gccgcagccc aggagccgcc ccaccgcagc tggcgatgga cccgccgagg
      421 cccgcgctgc tggcgctgct ggcgctgcct gcgctgctgc tgctgctgct ggcgggcgcc
      481 agggccgaag aggaaatgct ggaaaatgtc agcctggtct gtccaaaaga tgcgacccga
      541 ttcaagcacc tccggaagta cacatacaac tatgaggctg agagttccag tggagtccct
      601 gggactgctg attcaagaag tgccaccagg atcaactgca aggttgagct ggaggttccc
      661 cagctctgca gcttcatcct gaagaccagc cagtgcaccc tgaaagaggt gtatggcttc
      721 aaccctgagg gcaaagcctt gctgaagaaa accaagaact ctgaggagtt tgctgcagcc
      781 atgtccaggt atgagctcaa gctggccatt ccagaaggga agcaggtttt cctttacccg
      841 gagaaagatg aacctactta catcctgaac atcaagaggg gcatcatttc tgccctcctg
      901 gttcccccag agacagaaga agccaagcaa gtgttgtttc tggataccgt gtatggaaac
      961 tgctccactc actttaccgt caagacgagg aagggcaatg tggcaacaga aatatccact
     1021 gaaagagacc tggggcagtg tgatcgcttc aagcccatcc gcacaggcat cagcccactt
     1081 gctctcatca aaggcatgac ccgccccttg tcaactctga tcagcagcag ccagtcctgt
     1141 cagtacacac tggacgctaa gaggaagcat gtggcagaag ccatctgcaa ggagcaacac
     1201 ctcttcctgc ctttctccta caagaataag tatgggatgg tagcacaagt gacacagact
     1261 ttgaaacttg aagacacacc aaagatcaac agccgcttct ttggtgaagg tactaagaag
     1321 atgggcctcg catttgagag caccaaatcc acatcacctc caaagcaggc cgaagctgtt
     1381 ttgaagactc tccaggaact gaaaaaacta accatctctg agcaaaatat ccagagagct
     1441 aatctcttca ataagctggt tactgagctg agaggcctca gtgatgaagc agtcacatct
     1501 ctcttgccac agctgattga ggtgtccagc cccatcactt tacaagcctt ggttcagtgt
     1561 ggacagcctc agtgctccac tcacatcctc cagtggctga aacgtgtgca tgccaacccc
     1621 cttctgatag atgtggtcac ctacctggtg gccctgatcc ccgagccctc agcacagcag
     1681 ctgcgagaga tcttcaacat ggcgagggat cagcgcagcc gagccacctt gtatgcgctg
     1741 agccacgcgg tcaacaacta tcataagaca aaccctacag ggacccagga gctgctggac
     1801 attgctaatt acctgatgga acagattcaa gatgactgca ctggggatga agattacacc
     1861 tatttgattc tgcgggtcat tggaaatatg ggccaaacca tggagcagtt aactccagaa
     1921 ctcaagtctt caatcctgaa atgtgtccaa agtacaaagc catcactgat gatccagaaa
     1981 gctgccatcc aggctctgcg gaaaatggag cctaaagaca aggaccagga ggttcttctt
     2041 cagactttcc ttgatgatgc ttctccggga gataagcgac tggctgccta tcttatgttg
     2101 atgaggagtc cttcacaggc agatattaac aaaattgtcc aaattctacc atgggaacag
     2161 aatgagcaag tgaagaactt tgtggcttcc catattgcca atatcttgaa ctcagaagaa
     2221 ttggatatcc aagatctgaa aaagttagtg aaagaagctc tgaaagaatc tcaacttcca
     2281 actgtcatgg acttcagaaa attctctcgg aactatcaac tctacaaatc tgtttctctt
     2341 ccatcacttg acccagcctc agccaaaata gaagggaatc ttatatttga tccaaataac
     2401 taccttccta aagaaagcat gctgaaaact accctcactg cctttggatt tgcttcagct
     2461 gacctcatcg agattggctt ggaaggaaaa ggctttgagc caacattgga agctcttttt
     2521 gggaagcaag gatttttccc agacagtgtc aacaaagctt tgtactgggt taatggtcaa
     2581 gttcctgatg gtgtctctaa ggtcttagtg gaccactttg gctataccaa agatgataaa
     2641 catgagcagg atatggtaaa tggaataatg ctcagtgttg agaagctgat taaagatttg
     2701 aaatccaaag aagtcccgga agccagagcc tacctccgca tcttgggaga ggagcttggt
     2761 tttgccagtc tccatgacct ccagctcctg ggaaagctgc ttctgatggg tgcccgcact
     2821 ctgcagggga tcccccagat gattggagag gtcatcagga agggctcaaa gaatgacttt
     2881 tttcttcact acatcttcat ggagaatgcc tttgaactcc ccactggagc tggattacag
     2941 ttgcaaatat cttcatctgg agtcattgct cccggagcca aggctggagt aaaactggaa
     3001 gtagccaaca tgcaggctga actggtggca aaaccctccg tgtctgtgga gtttgtgaca
     3061 aatatgggca tcatcattcc ggacttcgct aggagtgggg tccagatgaa caccaacttc
     3121 ttccacgagt cgggtctgga ggctcatgtt gccctaaaag ctgggaagct gaagtttatc
     3181 attccttccc caaagagacc agtcaagctg ctcagtggag gcaacacatt acatttggtc
     3241 tctaccacca aaacggaggt gatcccacct ctcattgaga acaggcagtc ctggtcagtt
     3301 tgcaagcaag tctttcctgg cctgaattac tgcacctcag gcgcttactc caacgccagc
     3361 tccacagact ccgcctccta ctatccgctg accggggaca ccagattaga gctggaactg
     3421 aggcctacag gagagattga gcagtattct gtcagcgcaa cctatgagct ccagagagag
     3481 gacagagcct tggtggatac cctgaagttt gtaactcaag cagaaggtgc gaagcagact
     3541 gaggctacca tgacattcaa atataatcgg cagagtatga ccttgtccag tgaagtccaa
     3601 attccggatt ttgatgttga cctcggaaca atcctcagag ttaatgatga atctactgag
     3661 ggcaaaacgt cttacagact caccctggac attcagaaca agaaaattac tgaggtcgcc
     3721 ctcatgggcc acctaagttg tgacacaaag gaagaaagaa aaatcaaggg tgttatttcc
     3781 ataccccgtt tgcaagcaga agccagaagt gagatcctcg cccactggtc gcctgccaaa
     3841 ctgcttctcc aaatggactc atctgctaca gcttatggct ccacagtttc caagagggtg
     3901 gcatggcatt atgatgaaga gaagattgaa tttgaatgga acacaggcac caatgtagat
     3961 accaaaaaaa tgacttccaa tttccctgtg gatctctccg attatcctaa gagcttgcat
     4021 atgtatgcta atagactcct ggatcacaga gtccctcaaa cagacatgac tttccggcac
     4081 gtgggttcca aattaatagt tgcaatgagc tcatggcttc agaaggcatc tgggagtctt
     4141 ccttataccc agactttgca agaccacctc aatagcctga aggagttcaa cctccagaac
     4201 atgggattgc cagacttcca catcccagaa aacctcttct taaaaagcga tggccgggtc
     4261 aaatatacct tgaacaagaa cagtttgaaa attgagattc ctttgccttt tggtggcaaa
     4321 tcctccagag atctaaagat gttagagact gttaggacac cagccctcca cttcaagtct
     4381 gtgggattcc atctgccatc tcgagagttc caagtcccta cttttaccat tcccaagttg
     4441 tatcaactgc aagtgcctct cctgggtgtt ctagacctct ccacgaatgt ctacagcaac
     4501 ttgtacaact ggtccgcctc ctacagtggt ggcaacacca gcacagacca tttcagcctt
     4561 cgggctcgtt accacatgaa ggctgactct gtggttgacc tgctttccta caatgtgcaa
     4621 ggatctggag aaacaacata tgaccacaag aatacgttca cactatcata tgatgggtct
     4681 ctacgccaca aatttctaga ttcgaatatc aaattcagtc atgtagaaaa acttggaaac
     4741 aacccagtct caaaaggttt actaatattc gatgcatcta gttcctgggg accacagatg
     4801 tctgcttcag ttcatttgga ctccaaaaag aaacagcatt tgtttgtcaa agaagtcaag
     4861 attgatgggc agttcagagt ctcttcgttc tatgctaaag gcacatatgg cctgtcttgt
     4921 cagagggatc ctaacactgg ccggctcaat ggagagtcca acctgaggtt taactcctcc
     4981 tacctccaag gcaccaacca gataacagga agatatgaag atggaaccct ctccctcacc
     5041 tccacctctg atctgcaaag tggcatcatt aaaaatactg cttccctaaa gtatgagaac
     5101 tacgagctga ctttaaaatc tgacaccaat gggaagtata agaactttgc cacttctaac
     5161 aagatggata tgaccttctc taagcaaaat gcactgctgc gttctgaata tcaggctgat
     5221 tacgagtcat tgaggttctt cagcctgctt tctggatcac taaattccca tggtcttgag
     5281 ttaaatgctg acatcttagg cactgacaaa attaatagtg gtgctcacaa ggcgacacta
     5341 aggattggcc aagatggaat atctaccagt gcaacgacca acttgaagtg tagtctcctg
     5401 gtgctggaga atgagctgaa tgcagagctt ggcctctctg gggcatctat gaaattaaca
     5461 acaaatggcc gcttcaggga acacaatgca aaattcagtc tggatgggaa agccgccctc
     5521 acagagctat cactgggaag tgcttatcag gccatgattc tgggtgtcga cagcaaaaac
     5581 attttcaact tcaaggtcag tcaagaagga cttaagctct caaatgacat gatgggctca
     5641 tatgctgaaa tgaaatttga ccacacaaac agtctgaaca ttgcaggctt atcactggac
     5701 ttctcttcaa aacttgacaa catttacagc tctgacaagt tttataagca aactgttaat
     5761 ttacagctac agccctattc tctggtaact actttaaaca gtgacctgaa atacaatgct
     5821 ctggatctca ccaacaatgg gaaactacgg ctagaacccc tgaagctgca tgtggctggt
     5881 aacctaaaag gagcctacca aaataatgaa ataaaacaca tctatgccat ctcttctgct
     5941 gccttatcag caagctataa agcagacact gttgctaagg ttcagggtgt ggagtttagc
     6001 catcggctca acacagacat cgctgggctg gcttcagcca ttgacatgag cacaaactat
     6061 aattcagact cactgcattt cagcaatgtc ttccgttctg taatggcccc gtttaccatg
     6121 accatcgatg cacatacaaa tggcaatggg aaactcgctc tctggggaga acatactggg
     6181 cagctgtata gcaaattcct gttgaaagca gaacctctgg catttacttt ctctcatgat
     6241 tacaaaggct ccacaagtca tcatctcgtg tctaggaaaa gcatcagtgc agctcttgaa
     6301 cacaaagtca gtgccctgct tactccagct gagcagacag gcacctggaa actcaagacc
     6361 caatttaaca acaatgaata cagccaggac ttggatgctt acaacactaa agataaaatt
     6421 ggcgtggagc ttactggacg aactctggct gacctaactc tactagactc cccaattaaa
     6481 gtgccacttt tactcagtga gcccatcaat atcattgatg ctttagagat gagagatgcc
     6541 gttgagaagc cccaagaatt tacaattgtt gcttttgtaa agtatgataa aaaccaagat
     6601 gttcactcca ttaacctccc attttttgag accttgcaag aatattttga gaggaatcga
     6661 caaaccatta tagttgtact ggaaaacgta cagagaaacc tgaagcacat caatattgat
     6721 caatttgtaa gaaaatacag agcagccctg ggaaaactcc cacagcaagc taatgattat
     6781 ctgaattcat tcaattggga gagacaagtt tcacatgcca aggagaaact gactgctctc
     6841 acaaaaaagt atagaattac agaaaatgat atacaaattg cattagatga tgccaaaatc
     6901 aactttaatg aaaaactatc tcaactgcag acatatatga taggcaccag cggcggttca
     6961 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     7021 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     7081 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     7141 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     7201 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     7261 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     7321 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     7381 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     7441 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     7501 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     7561 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     7621 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
     7681 acccagcttt cttgtacaaa gtggtgatgg ccggccgctt cgagcagaca tgataagata
     7741 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     7801 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     7861 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     7921 caagtaaaac ctctacaaat gtggtaaaat cgataaggat ccgtcgaccg atgcccttga
     7981 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac
     8041 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctcttccgct
     8101 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     8161 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     8221 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     8281 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     8341 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     8401 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     8461 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     8521 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     8581 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     8641 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     8701 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     8761 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     8821 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     8881 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     8941 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     9001 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     9061 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     9121 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     9181 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     9241 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     9301 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     9361 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     9421 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     9481 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     9541 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     9601 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     9661 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     9721 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     9781 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     9841 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     9901 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     9961 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    10021 cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
    10081 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
    10141 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
    10201 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
    10261 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
    10321 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
    10381 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
    10441 tttaacgcga attttaacaa aatattaacg cttacaattt gccattcgcc attcaggctg
    10501 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gcccaagcta
    10561 ccatgataag taagtaatat taaggtacgg gaggtacttg gagcggccgc aataaaatat
    10621 ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgatagta ctaacatacg
    10681 ctctccatca aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc cccagtgcaa
    10741 gtgcaggtgc cagaacattt ctctatcgat aggtaccgag ctcttacgcg tgctagcatc
    10801 aactttgtat agaaaagttg tagttattaa tagtaatcaa ttacggggtc attagttcat
    10861 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
    10921 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
    10981 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
    11041 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
    11101 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
    11161 gtattagtca tcgctattac catg
//
