LOCUS       sequence-272357-        8311 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses MTP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8311)
  AUTHORS   Rava P, Ojakian GK, Shelness GS, Hussain MM
  TITLE     Phospholipid transfer activity of microsomal triacylglycerol 
            transfer protein is sufficient for the assembly and secretion of 
            apolipoprotein B lipoproteins.
  JOURNAL   J Biol Chem. 2006 Apr 21;281(16):11019-27. doi: 
            10.1074/jbc.M512823200. Epub 2006 Feb 13.
  PUBMED    16478722
REFERENCE   2  (bases 1 to 8311)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M512823200"; journalName: "J Biol Chem"; date: 
            "2006-04-21- 21"; volume: "281"; issue: "16"; pages: "11019-27"
COMMENT     Sequence Label: hMTP-flag
FEATURES             Location/Qualifiers
     source          1..8311
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3453..3476
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(3510..3526)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3510..3526)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(3536..3554)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3537..3554)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3574..3591)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3580..3804
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3850..4363
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3937..3956)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4147..4168
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4386..4406)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4391..4720
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4571..4706
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4787..5581
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4841..4860)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5451..5470
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5755..5889
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5792..5811)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5846..5865
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5920..5936)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5920..5936)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5933..5955)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5944..5960
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5968..5974,5975..5992,5993..5998))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6013..6034
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6151..6168)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6322..6910)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6402..6421)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7081..7872,7873..7941))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7704..7723
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7942..8046)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8121..8140)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatcccc cgggctgcag gaattcggca cgagtgaaag agtccacttc
      721 tcagtgactc ctagctgggc actggatgca gttgaggatt gctggtcaat atgattcttc
      781 ttgctgtgct ttttctctgc ttcatttcct catattcagc ttctgttaaa ggtcacacaa
      841 ctggtctctc attaaataat gaccggctgt acaagctcac gtactccact gaagttcttc
      901 ttgatcgggg caaaggaaaa ctgcaagaca gcgtgggcta ccgcatttcc tccaacgtgg
      961 atgtggcctt actatggagg aatcctgatg gtgatgatga ccagttgatc caaataacga
     1021 tgaaggatgt aaatgttgaa aatgtgaatc agcagagagg agagaagagc atcttcaaag
     1081 gaaaaagccc atctaaaata atgggaaagg aaaacttgga agctctgcaa agacctacgc
     1141 tccttcatct aatccatgga aaggtcaaag agttctactc atatcaaaat gaggcagtgg
     1201 ccatagaaaa tatcaagaga ggtctggcta gcctatttca gacacagtta agctctggaa
     1261 ccaccaatga ggtagatatc tctggaaatt gtaaagtgac ctaccaggct catcaagaca
     1321 aagtgatcaa aattaaggcc ttggattcat gcaaaatagc gaggtctgga tttacgaccc
     1381 caaatcaggt cttgggtgtc agttcaaaag ctacatctgt caccacctat aagatagaag
     1441 acagctttgt tatagctgtg cttgctgaag aaacacacaa ttttggactg aatttcctac
     1501 aaaccattaa ggggaaaata gtatcgaagc agaaattaga gctgaagaca accgaagcag
     1561 gcccaagatt gatgtctgga aagcaggctg cagccataat caaagcagtt gattcaaagt
     1621 acacggccat tcccattgtg gggcaggtct tccagagcca ctgtaaagga tgtccttctc
     1681 tctcggagct ctggcggtcc accaggaaat acctgcagcc tgacaacctt tccaaggctg
     1741 aggctgtcag aaacttcctg gccttcattc agcacctcag gactgcgaag aaagaagaga
     1801 tccttcaaat actaaagatg gaaaataagg aagtattacc tcagctggtg gatgctgtca
     1861 cctctgctca gacctcagac tcattagaag ccattttgga ctttttggat ttcaaaagtg
     1921 acagcagcat tatcctccag gagaggtttc tctatgcctg tggatttgct tctcatccca
     1981 atgaagaact cctgagagcc ctcattagta agttcaaagg ttctattggt agcagtgaca
     2041 tcagagaaac tgttatgatc atcactggga cacttgtcag aaagttgtgt cagaatgaag
     2101 gctgcaaact caaagcagta gtggaagcta agaagttaat cctgggagga cttgaaaaag
     2161 cagagaaaaa agaggacacc aggatgtatc tgctggcttt gaagaatgcc ctgcttccag
     2221 aaggcatccc aagtcttctg aagtatgcag aagcaggaga agggcccatc agccacctgg
     2281 ctaccactgc tctccagaga tatgatctcc ctttcataac tgatgaggtg aagaagacct
     2341 taaacagaat ataccaccaa aaccgtaaag ttcatgaaaa gactgtgcgc actgctgcag
     2401 ctgctatcat tttaaataac aatccatcct acatggacgt caagaacatc ctgctgtcta
     2461 ttggggagct tccccaagaa atgaataaat acatgctcgc cattgttcaa gacatcctac
     2521 gttttgaaat gcctgcaagc aaaattgtcc gtcgagttct gaaggaaatg gtcgctcaca
     2581 attatgaccg tttctccagg agtggatctt cttctgccta cactggctac atagaacgta
     2641 gtccccgttc ggcatctact tacagcctag acattctcta ctcgggttct ggcattctaa
     2701 ggagaagtaa cctgaacatc tttcagtaca ttgggaaggc tggtcttcac ggtagccagg
     2761 tggttattga agcccaagga ctggaagcct taatcgcagc cacccctgac gagggggagg
     2821 agaaccttga ctcctatgct ggtatgtcag ccatcctctt tgatgttcag ctcagacctg
     2881 tcaccttttt caacggatac agtgatttga tgtccaaaat gctgtcagca tctggcgacc
     2941 ctatcagtgt ggtgaaagga cttattctgc taatagatca ttctcaggaa cttcagttac
     3001 aatctggact aaaagccaat atagaggtcc agggtggtct agctattgat atttcaggtg
     3061 caatggagtt tagcttgtgg tatcgtgagt ctaaaacccg agtgaaaaat agggtgactg
     3121 tggtaataac cactgacatc acagtggact cctcttttgt gaaagctggc ctggaaacca
     3181 gtacagaaac agaagcaggc ttggagttta tctccacagt gcagttttct cagtacccat
     3241 tcttagtttg catgcagatg gacaaggatg aagctccatt caggcaattt gagaaaaagt
     3301 acgaaaggct gtccacaggc agaggttatg tctctcagaa aagaaaagaa agcgtattag
     3361 caggatgtga attcccgctc catcaagaga actcagagat gtgcaaagtg gtgtttgccc
     3421 ctcagccgga tagtacttcc agcggatggt ttgactacaa ggacgatgac gacaagtgat
     3481 ctagagcatc gaattcctgc agcccggggg atccactagt tctagagggc cctattctat
     3541 agtgtcacct aaatgctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc
     3601 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     3661 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     3721 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     3781 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctcgaggg
     3841 gggatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     3901 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     3961 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     4021 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     4081 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     4141 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     4201 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     4261 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaaata tttgcttata
     4321 caatcttcct gtttttgggg cttttctgat tatcaaccgg ggtgggtacc gagctcgaat
     4381 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     4441 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     4501 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     4561 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     4621 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     4681 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttggat
     4741 atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     4801 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     4861 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     4921 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
     4981 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     5041 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     5101 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     5161 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     5221 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     5281 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
     5341 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     5401 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     5461 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     5521 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     5581 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
     5641 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     5701 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     5761 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     5821 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     5881 gtctggatcc gtcgacctcg agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     5941 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     6001 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     6061 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     6121 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     6181 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     6241 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     6301 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     6361 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     6421 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     6481 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     6541 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     6601 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     6661 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     6721 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     6781 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     6841 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     6901 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     6961 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     7021 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     7081 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     7141 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     7201 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     7261 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     7321 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     7381 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     7441 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     7501 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     7561 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     7621 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     7681 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     7741 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     7801 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     7861 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     7921 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     7981 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     8041 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
     8101 cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
     8161 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
     8221 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
     8281 cttcgcgatg tacgggccag atatacgcgt t
//
