LOCUS       sequence-272361-        9304 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline-inducible vector backbone containing a multiple cloning 
            site (MCS), the engrailed (EnR) repressor domain and a STOP codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9304)
  AUTHORS   Adrian-Segarra JM, Weigel B, Mall M
  TITLE     Combining Cell Fate Reprogramming and Protein Engineering to Study 
            Transcription Factor Functions.
  JOURNAL   Methods Mol Biol. 2021;2352:227-236. doi: 
            10.1007/978-1-0716-1601-7_15.
  PUBMED    34324190
REFERENCE   2  (bases 1 to 9304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2021"; volume: "2352:227-236. doi"; pages: " 
            10.1007/978-1-0716-1601-7_15"
COMMENT     Sequence Label: TetO-FUW-MCS-EnR_C-term
FEATURES             Location/Qualifiers
     source          1..9304
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    927..1515
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(980..1000)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1398..1409)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1518..1534)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1519..1535)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2040..2220
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2246..2263)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2252..2476
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2522..2950
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2609..2628)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2819..2840
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2959..2979)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2964..3293
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3144..3279
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3206..3225
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3341..3388
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3407..3781
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    3911..4032
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3948..3967)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4002..4021
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4081..4097)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4081..4097)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4094..4116)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4105..4121
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4129..4135,4136..4153,4154..4159))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4174..4195
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4312..4329)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4483..5071)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4563..4582)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5242..6033,6034..6102))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5865..5884
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6103..6207)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6282..6301)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6473..6852
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6854..7052
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             7070..7250
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7297..7422
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7915..8148
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8333..8377
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8675..8792
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(8849..8867,8868..8890,8891..8909,8910..8932,8933..8951,
                     8952..8974,8975..8993,8994..9016,9017..9035,9036..9058,
                     9059..9077,9078..9100,9101..9119)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        9152..9190
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9187..9211
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ctagaatggc cctggaggat cgctgcagcc cacagtcagc gcccagcccc attaccctac
       61 aaatgcagca tcttcaccac cagcaacagc agcagcagca acagcagcag caaatgcagc
      121 acctccacca gctgcagcaa ctgcagcagt tgcaccaaca gcaactggcc gccggtgtct
      181 tccaccatcc ggcaatggcc ttcgatgccg ctgcagccgc cgctgctgca gctgctgctg
      241 cggccgccca cgctcatgct gctgcactgc agcagcgcct cagtggcagt ggatcgcccg
      301 catcctgctc cacgcccgcc tcgtccacgc cgctgaccat caaggaggag gaaagcgact
      361 ccgtgatcgg tgacatgagt ttccacaatc agacgcacac caccaacgag gaggaggagg
      421 cggaggagga tgacgacatt gatgtggatg tggatgatac gtcggcgggc ggacgcctgc
      481 caccacccgc ccaccagcag cagtcgacgg ccaagccctc gctggccttt tccatctcca
      541 acatcctgag cgatcgtttc ggagatgtcc agaagcctgg caagtcgatt gagaaccagg
      601 ccagcatatt ccgccccttc gaggcgaatc gctcccagac tgccacgccc tccgccttta
      661 caagagtgga tctgctggag tttagccggc aacagcaagc tgccgccgca gccgctactg
      721 cggccatgat gctggaacgg gccaacttcc ttaactgctt caatccggct gcctatccca
      781 ggatacacga ggaaatcgtg cagagtcgcc tgcgcaggag tgcagccaat gccgtcatcc
      841 cgccgcccat gagctccaag atgagcgatg ccaatccaga gaaatctgct ctgtgagcta
      901 gctaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat
      961 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     1021 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     1081 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     1141 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     1201 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     1261 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     1321 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     1381 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     1441 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     1501 gggccgcctc cccgcatcga taccgtcgac ctcgagacct agaaaaacat ggagcaatca
     1561 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
     1621 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
     1681 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     1741 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     1801 ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca
     1861 agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct
     1921 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga
     1981 ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg
     2041 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     2101 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     2161 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     2221 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     2281 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     2341 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     2401 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     2461 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     2521 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     2581 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     2641 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     2701 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     2761 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     2821 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     2881 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     2941 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     3001 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     3061 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     3121 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     3181 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     3241 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     3301 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc
     3361 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac
     3421 cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga
     3481 ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga
     3541 cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg
     3601 ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa
     3661 cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga
     3721 gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg
     3781 acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     3841 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     3901 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     3961 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     4021 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     4081 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     4141 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     4201 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     4261 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     4321 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     4381 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     4441 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     4501 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     4561 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     4621 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     4681 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     4741 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     4801 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     4861 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     4921 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     4981 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     5041 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     5101 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     5161 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     5221 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     5281 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     5341 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     5401 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     5461 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     5521 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     5581 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     5641 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     5701 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     5761 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     5821 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     5881 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     5941 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     6001 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     6061 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     6121 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     6181 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
     6241 cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc
     6301 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
     6361 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
     6421 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga
     6481 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     6541 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     6601 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     6661 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     6721 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     6781 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     6841 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     6901 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     6961 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     7021 aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt gcctgtactg ggtctctctg
     7081 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7141 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7201 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     7261 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     7321 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     7381 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     7441 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     7501 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     7561 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     7621 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     7681 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     7741 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     7801 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     7861 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     7921 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     7981 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     8041 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     8101 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     8161 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     8221 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     8281 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     8341 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     8401 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     8461 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     8521 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     8581 tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc
     8641 aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     8701 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     8761 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     8821 cagtttggtt agatctcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     8881 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
     8941 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
     9001 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc
     9061 cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa
     9121 aagtgaaagt cgagctcggt acccgggtcg aggtaggcgt gtacggtggg aggcctatat
     9181 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     9241 cctccataga agacaccggg accgatccag cctccgcggc cccgaattcg aagactagga
     9301 tcct
//
