LOCUS       sequence-272390-        2926 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses MobR-regulated 3WJdB RNA aptamer driven by T7 promoter 
            with the mobO sequence 5BP downstream from the promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Jung JK, Alam KK, Verosloff MS, Capdevila DA, Desmau M, Clauer PR, 
            Lee JW, Nguyen PQ, Pasten PA, Matiasek SJ, Gaillard JF, Giedroc DP, 
            Collins JJ, Lucks JB
  TITLE     Cell-free biosensors for rapid detection of water contaminants.
  JOURNAL   Nat Biotechnol. 2020 Jul 6. pii: 10.1038/s41587-020-0571-7. doi: 
            10.1038/s41587-020-0571-7.
  PUBMED    32632301
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Jul 6. pii: 10.1038/s41587-020-0571-7. doi: 
            10.1038/s41587-020-0571-7."
COMMENT     Sequence Label: pJBL718
FEATURES             Location/Qualifiers
     source          1..2926
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        263..281
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      458..504
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(530..547)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(530..546)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(539..561)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(755..774)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     874..896
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(934..952)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1020..1124
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1125..1193,1194..1985)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1343..1362)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2156..2744
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2645..2664
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2898..2915
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
      241 atgcctgcag gtcgactcta gataatacga ctcactatag gaggtactat ttgtgtgcgg
      301 actgacccac atactctgat gatccgagac ggtcgggtcc agatattcgt atctgtcgag
      361 tagagtgtgg gctcggatca ttcatggcaa gagacggtcg ggtccagata ttcgtatctg
      421 tcgagtagag tgtgggctct tgccatgtgt atgtgggtag cataacccct tggggcctct
      481 aaacgggtct tgaggggttt tttgatcccc gggtaccgag ctcgaattca ctggccgtcg
      541 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
      601 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
      661 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
      721 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
      781 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
      841 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
      901 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
      961 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     1021 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     1081 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     1141 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     1201 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     1261 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     1321 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     1381 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     1441 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     1501 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     1561 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     1621 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     1681 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     1741 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     1801 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     1861 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     1921 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     1981 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     2041 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     2101 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     2161 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     2221 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     2281 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     2341 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     2401 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     2461 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     2521 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     2581 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     2641 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     2701 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     2761 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     2821 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     2881 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaaga
//
