LOCUS       sequence-27247-e         684 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Yuasa T, Ohno S, Kehrl JH, Kyriakis JM
  TITLE     Tumor necrosis factor signaling to stress-activated protein kinase 
            (SAPK)/Jun NH2-terminal kinase (JNK) and p38. Germinal center kinase
            couples TRAF2 to mitogen-activated protein kinase/ERK kinase kinase 
            1 and SAPK while receptor interacting protein associates with a 
            mitogen-activated protein kinase kinase kinase upstream of MKK6 and 
            p38.
  JOURNAL   J Biol Chem. 1998 Aug 28. 273(35):22681-92.
  PUBMED    9712898
REFERENCE   2  (bases 1 to 684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1998 Aug 28. 273(35):22681-92."
COMMENT     Sequence Label: pCMV5-FLAG-MKK6
FEATURES             Location/Qualifiers
     source          1..684
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      25..34
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..57
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctcgtttagt gaaccgtcag aattgccacc atggactaca aggacgacga tgacaagaat
       61 tctgcaggat cccgggtacc tctagagatg tctcagtcga aaggcaagaa gcgaaaccct
      121 ggccttaaaa ttccaaaaga agcatttgaa caacctcaga ccagttccac accacctcga
      181 gatttagact ccaaggcttg catttctatt ggaaatcaga actttgaggt gaaggcagat
      241 gacctggagc ctataatgga actgggacga ggtgcgtacg gggtggtgga gaagatgcgg
      301 cacgtgccca gcgggcagat catggcagtg aagcggatcc gagccacagt aaatagccag
      361 gaacagaaac ggctactgat ggatttggat atttccatga ggacggtgga ctgtccattc
      421 actgtcacct tttatggcgc actgtttcgg gagggtgatg tgtggatctg catggagctc
      481 atggatacat cactagataa attctacaaa caagttattg ataaaggcca gacaattcca
      541 gaggacatct tagggaaaat agcagtttct attgtaaaag cattagaaca tttacatagt
      601 aagctgtctg tcattcacag agacgtcaag ccttctaatg tactcatcaa tgctctcggt
      661 caagtgaaga tgtgcgattt tgga
//
