LOCUS       sequence-272476-        5612 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression of CENP-U and CENP-Q.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   McKinley KL, Sekulic N, Guo LY, Tsinman T, Black BE, Cheeseman IM
  TITLE     The CENP-L-N Complex Forms a Critical Node in an Integrated Meshwork
            of Interactions at the Centromere-Kinetochore Interface.
  JOURNAL   Mol Cell. 2015 Dec 17;60(6):886-98. doi: 
            10.1016/j.molcel.2015.10.027. Epub 2015 Nov 19.
  PUBMED    26698661
REFERENCE   2  (bases 1 to 5612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.10.027"; journalName: "Mol Cell"; date: 
            "2015-12-17- 17"; volume: "60"; issue: "6"; pages: "886-98"
COMMENT     Sequence Label: pKM463
FEATURES             Location/Qualifiers
     source          1..5612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(18..23,24..40,41..46))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(117..150)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        227..318
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             364..384
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     polyA_signal    1854..1988
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2160..2251
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    3285..3419
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  3540..3705
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3895..4483
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4384..4403
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  4618..4841
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4908..5441)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 tacgctactt gcattacagt ttacgaaccg aacaggctta tgtcaactgg gttcgtgcct
       61 tcatccgttt ccacggtgtg cgtcacccgg caaccttggg cagcagcgaa gtcgccataa
      121 cttcgtatag catacattat acgaagttat ctgtaactat aacggtccta aggtagcgag
      181 tttaaacact agtatcgatt cgcgacctac tccggaatat taatagatca tggagataat
      241 taaaatgata accatctcgc aaataaataa gtattttact gttttcgtaa cagttttgta
      301 ataaaaaaac ctataaatat tccggattat tcataccgtc ccaccatcgg gcgcggatcc
      361 atgcatcatc atcatcatca tcatggcagc ggcagcatgg ccccgcgcgg tcgtcgccgt
      421 cctcgtccgc accgtagcga aggtgcccgt cgtagcaaga acaccctgga gcgcacccac
      481 agcatgaagg acaaggccgg tcagaagtgc aaacctatcg acgtgttcga cttcccggac
      541 aacagcgacg tgagtagtat cggccgcctg ggtgagaatg agaaggacga ggagacctac
      601 gagacattcg atccgcctct gcatagcacc gccatttacg cagacgaaga ggagttcagc
      661 aagcactgcg gcctgagctt aagcagcacc ccgcctggca aggaagcaaa gcgtagcagt
      721 gacaccagtg gcaatgaggc cagtgaaatc gagagcgtga agatcagcgc aaagaagcct
      781 ggccgtaagc tgcgcccgat cagcgacgat agcgagagca ttgaggagag tgatacccgc
      841 cgcaaggtga aaagcgcaga gaagatcagc acccagcgcc acgaagttat tcgcaccaca
      901 gcaagcagcg aactgagcga gaaaccggcc gaaagcgtga caagtaaaaa gacaggcccg
      961 ctgagcgcac aacctagcgt ggaaaaggag aacctggcca tcgaaagcca gagtaagacc
     1021 caaaaaaaag gtaagattag ccatgacaag cgcaagaaga gccgtagcaa ggccatcggc
     1081 agcgacacca gtgacatcgt gcacatttgg tgcccggagg gtatgaaaac cagcgacatt
     1141 aaagagctga acatcgtgct gccggagttt gagaagaccc acctggaaca ccagcagcgc
     1201 atcgagagta aggtgtgcaa ggccgccatc gccacctttt acgttaatgt taaagagcag
     1261 tttattaaaa tgctgaagga gagccagatg ctgaccaacc tgaagcgcaa gaacgccaag
     1321 atgatcagcg acatcgagaa gaagcgccag cgcatgatcg aggtgcagga cgaattactg
     1381 cgcctggagc cgcagctgaa gcaactgcag acaaagtacg acgagctgaa ggagcgcaag
     1441 agcagcctgc gcaacgcagc ctactttctg agcaacctga aacagttata tcaagattac
     1501 agcgatgtgc aggcacagga gccgaatgtg aaagagacat acgacagcag tagtctgcct
     1561 gccttactgt tcaaggcccg taccctgtta ggtgcagaga gccatctgcg caacatcaac
     1621 caccagctgg aaaagttact ggaccagggc taagaattca aaggcctacg tcgacgagct
     1681 cacttgtcgc ggccgctttc gaatctagag cctgcagtct cgacaagctt gtcgagaagt
     1741 actagaggat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     1801 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     1861 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     1921 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     1981 tctggatctg atcactgctt gagcctagaa gatccggctg ctaacaaagc ccgaaaggaa
     2041 gctgagttgg ctgctgccac cgctgagcaa taactatcat aacccctagg gtatacccat
     2101 ctaaggtagc gagtttaaac actagtatcg attcgcgacc tactccggaa tattaataga
     2161 tcatggagat aattaaaatg ataaccatct cgcaaataaa taagtatttt actgttttcg
     2221 taacagtttt gtaataaaaa aacctataaa tattccggat tattcatacc gtcccaccat
     2281 cgggcgcgga tccatgagcg gcaaggccaa cgccagcaag aagaatgccc agcagctgaa
     2341 gcgcaatccg aagcgtaaga aggacaacga agaggtggtg ctgagcgaga acaaggtgcg
     2401 caacaccgtg aagaagaata aaaaccacct gaaagacctg agcagcgagg gccagaccaa
     2461 gcatacaaac ctgaagcacg gcaagaccgc cgccagcaag cgtaaaacct ggcagcctct
     2521 gagcaagagc acccgtgacc acctgcagac catgatggag agcgtgatca tgaccatcct
     2581 gagtaacagc atcaaggaga aagaggagat ccagtatcat ctgaacttcc tgaagaagcg
     2641 tctgctgcaa cagtgcgaga ccctgaaggt gccgccgaag aagatggagg atctgaccaa
     2701 cgtgagcagc ctgctgaaca tggagcgcgc acgcgacaag gcaaacgaag aggggctggc
     2761 cctgctgcaa gaggagatcg acaagatggt ggagaccaca gagctgatga ccggcaacat
     2821 ccaaagcctg aagaacaaga tccagatcct ggccagcgag gttgaagaag aagaggagcg
     2881 cgtgaaacag atgcaccaga tcaacagcag cggcgtgctg agcttaccgg aactgagcca
     2941 gaaaaccctg aaggcaccga ccctgcagaa ggaaatcctg gccctgattc cgaaccagaa
     3001 cgccctgctg aaggacctgg acatcctgca caacagcagc cagatgaaga gcatgagcac
     3061 attcatcgag gaagcctaca agaagctgga cgccagttaa gtcgacgagc tcacttgtcg
     3121 cggccgcttt cgaatctaga gcctgcagtc tcgacaagct tgtcgagaag tactagagga
     3181 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     3241 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     3301 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     3361 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct
     3421 gatcactgct tgagcctaga agatccggct gctaacaaag cccgaaagga agctgagttg
     3481 gctgctgcca ccgctgagca ataactatca taacccctag ggtataccca tctaattgga
     3541 accagataag tgaaatctag ttccaaacta ttttgtcatt tttaattttc gtattagctt
     3601 acgacgctac acccagttcc catctatttt gtcactcttc cctaaataat ccttaaaaac
     3661 tccatttcca cccctcccag ttcccaacta ttttgtccgc ccacaaccgg ttgacttggg
     3721 tcaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     3781 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     3841 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     3901 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     3961 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     4021 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     4081 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     4141 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     4201 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     4261 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     4321 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     4381 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     4441 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     4501 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     4561 ccctgattga cttgggtcgc tcttcctgtg gatgcgcaga tgccctgcgt aagcgggtgt
     4621 gggcggacaa taaagtctta aactgaacaa aatagatcta aactatgaca ataaagtctt
     4681 aaactagaca gaatagttgt aaactgaaat cagtccagtt atgctgtgaa aaagcatact
     4741 ggacttttgt tatggctaaa gcaaactctt cattttctga agtgcaaatt gcccgtcgta
     4801 ttaaagaggg gcgtggccaa gggcatgtaa agactatatt cgcggcgttg tgacaattta
     4861 ccgaacaact ccgcggccgg gaagccgatc tcggcttgaa cgaattgtta ggtggcggta
     4921 cttgggtcga tatcaaagtg catcacttct tcccgtatgc ccaactttgt atagagagcc
     4981 actgcgggat cgtcaccgta atctgcttgc acgtagatca cataagcacc aagcgcgttg
     5041 gcctcatgct tgaggagatt gatgagcgcg gtggcaatgc cctgcctccg gtgctcgccg
     5101 gagactgcga gatcatagat atagatctca ctacgcggct gctcaaactt gggcagaacg
     5161 taagccgcga gagcgccaac aaccgcttct tggtcgaagg cagcaagcgc gatgaatgtc
     5221 ttactacgga gcaagttccc gaggtaatcg gagtccggct gatgttggga gtaggtggct
     5281 acgtctccga actcacgacc gaaaagatca agagcagccc gcatggattt gacttggtca
     5341 gggccgagcc tacatgtgcg aatgatgccc atacttgagc cacctaactt tgttttaggg
     5401 cgactgccct gctgcgtaac atcgttgctg ctgcgtaaca tcgttgctgc tccataacat
     5461 caaacatcga cccacggcgt aacgcgcttg ctgcttggat gcccgaggca tagactgtac
     5521 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
     5581 ggtcaaggtt ctggaccagt tgcgtgagcg ca
//
