LOCUS       sequence-27248-e         694 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Yuasa T, Ohno S, Kehrl JH, Kyriakis JM
  TITLE     Tumor necrosis factor signaling to stress-activated protein kinase 
            (SAPK)/Jun NH2-terminal kinase (JNK) and p38. Germinal center kinase
            couples TRAF2 to mitogen-activated protein kinase/ERK kinase kinase 
            1 and SAPK while receptor interacting protein associates with a 
            mitogen-activated protein kinase kinase kinase upstream of MKK6 and 
            p38.
  JOURNAL   J Biol Chem. 1998 Aug 28. 273(35):22681-92.
  PUBMED    9712898
REFERENCE   2  (bases 1 to 694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1998 Aug 28. 273(35):22681-92."
COMMENT     Sequence Label: pCMV5-FLAG-MKK6-K82M
FEATURES             Location/Qualifiers
     source          1..694
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             26..49
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gtgaaccgtc agaattgcca ccatggacta caaggacgac gatgacaaga attctgcagg
       61 atcccgggta cctctagaga tgtctcagtc gaaaggcaag aagcgaaacc ctggccttaa
      121 aattccaaaa gaagcatttg aacaacctca gaccagttcc acaccacctc gagatttaga
      181 ctccaaggct tgcatttcta ttggaaatca gaactttgag gtgaaggcag atgacctgga
      241 gcctataatg gaactgggac gaggtgcgta cggggtggtg gagaagatgc ggcacgtgcc
      301 cagcgggcag atcatggcag tgatgcggat ccgagccaca gtaaatagcc aggaacagaa
      361 acggctactg atggatttgg atatttccat gaggacggtg gactgtccat tcactgtcac
      421 cttttatggc gcactgtttc gggagggtga tgtgtggatc tgcatggagc tcatggatac
      481 atcactagat aaattctaca aacaagttat tgataaaggc cagacaattc cagaggacat
      541 cttagggaaa atagcagttt ctattgtaaa agcattagaa catttacata gtaagctgtc
      601 tgtcattcac agagacgtca agccttctaa tgtactcatc aatgctctcg gtcaagtgaa
      661 gatgtgcgat tttggaatca gtggctactt ggtg
//
