LOCUS       sequence-272481-        4308 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA Kit # 1000000119 extension.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4308)
  TITLE     Lewin Lab Plasmids
REFERENCE   2  (bases 1 to 4308)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRDAX-VALENTIA-003   Lyn-Eos//BsaI
FEATURES             Location/Qualifiers
     source          1..4308
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        158..537
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        539..814
                     /label=chicken beta-actin promoter
     regulatory      834..843
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    1692..1813
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1729..1748)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1783..1802
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     terminator      2614..2643
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      2665..3252
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3153..3172
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3264..3291)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(join(3315..4106,4107..4175))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3938..3957
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4176..4278)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(join(4279..4308,1..2))
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 ctttctgcta tggaggtcag gtatgattta aaggtctcat aggtcttgta ttcagtctcg
       61 cactttagac atggtcttct gctgcccgtg gtcctctatt cgtcggatgt tctgtcgatt
      121 cttcctgtct atctggactg gatctgatat catcgtcgac attgattatt gactagttat
      181 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      241 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      301 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      361 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      421 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      481 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
      541 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
      601 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg ggggggggcg
      661 cgcgccaggc ggggcggggc ggggcgrggg gcggggcggg gcgaggcgga gaggtgcggc
      721 ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg
      781 gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgttgg gacgccacca
      841 tgggctgcat caagagcaag aggaaagacg tggaaaacgt cgagggcggc agctcagcga
      901 tcaaacccga catgaagatc aacctccgaa tggaaggcaa cgtaaacggc catcacttcg
      961 tgattgatgg cgatggcaca gggaagccat ttgaggggaa acaaagcatg gatctggagg
     1021 tgaaggaagg tggtcctctg ccttttgcct tcgacatact gactacagcc tttcactatg
     1081 ggaatcgcgt gtttgtcgag tatcccgacc atatacagga ttacttcaag cagagtttcc
     1141 cgaaaggcta tagctgggaa aggtccctca catttgagga tggagggatt tgcattgcaa
     1201 gaaacgacat caccatggaa ggagacacct tctacaacaa ggttcggttc catgggacaa
     1261 atttccccgc taatggtcct gtgatgcaga agaaaaccct gaagtgggaa ccatccactg
     1321 agaagatgta cgtgagagat ggagtactga cgggtgacat tcacgctgca ctgctgttgg
     1381 agggcaatgc ccactatcgg tgtgattttc gcactaccta caaggccaaa gagaaaggcg
     1441 tcaaactccc tggataccac cttgtcgatc attgcatcga gatcctttct cacgacaagg
     1501 actacaacaa ggtgaagctg tatgaacatg ccgttgctca ctctggattg ccagacaatg
     1561 caaggcgttc aggctctgat taaggcgcca ggtacatctc caatacggcg tcttgcatag
     1621 agaacacgat tagcacaccg gttacccacg tgatgcccga acggacccat gtcatgccag
     1681 agcgcactgg taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     1741 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     1801 tcaatgtatc ttatcatgtc tgtcgtcgaa ttcgagttgg cgcgcctgtc attctaaatc
     1861 tctctttcag cctaaagctt tttccccgta tccccccagg tgtctgcagg ctcaaagagc
     1921 agcgagaagc gttcagagga aagcgatccc gtgccacctt ccccgtgccc gggctgtccc
     1981 cgcacgctgc cggctcgggg atgcgggggg agcgccggac cggagcggag ccccgggcgg
     2041 ctcgctgctg ccccctagcg ggggagggac gtaattacat ccctgggggc tttgggaggg
     2101 ggctgtcccc gtgagctccc aggcgcgcct gtcattctaa atctctcttt cagcctaaag
     2161 ctttttcccc gtatcccccc aggtgtctgc aggctcaaag agcagcgaga agcgttcaga
     2221 ggaaagcgat cccgtgccac cttccccgtg cccgggctgt ccccgcacgc tgccggctcg
     2281 gggatgcggg gggagcgccg gaccggagcg gagccccggg cggctcgctg ctgcccccta
     2341 gcgggggagg gacgtaatta catccctggg ggctttgggg gggggctgtc cccgtgagct
     2401 cccaggcgcg ccaactcggt acctcacccg attctcatca tggagacaca tcaggacatc
     2461 tccaataccg ccatggaaac gcaccaagat gcgactgctc ttctgccagt gacggcattc
     2521 gtctgcaaga ttaacggcat acagaaagaa acgatgagac ccattcagga cgagcctcag
     2581 actccagcgt aactggactg caatcaactc actggctcac cttcacgggt gggcctttct
     2641 tcggtagaaa atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     2701 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     2761 tctttttccg aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     2821 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     2881 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     2941 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     3001 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     3061 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     3121 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     3181 gtcgggtttc gccacctctg acttgagcat cgatttttgt gatgctcgtc aggggggcgg
     3241 agcctatgga aaaacgccag caacgcagaa aggcccaccc gaaggtgagc caggtgatta
     3301 catttgggcc ctcattacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     3361 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat gcgggagggc
     3421 ttaccatctg gccccagtgc tgcaatgata ccgcgagaac cacgctcacc ggctccagat
     3481 ttatcagcaa taaaccagcc agccgggagg gccgagcgca gaagtgatcc tgcaacttta
     3541 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     3601 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     3661 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     3721 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgccagaag taagttggcc
     3781 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     3841 gtgagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     3901 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     3961 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gtaaactctc aaggatctta
     4021 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     4081 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     4141 ggaataaggg cgacacggaa atgttgaata ctcattttag cttccttagc tcctgaaaat
     4201 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
     4261 cctcttacgt gccgatcaag tcaaaagcct ccggtcggag gcttttga
//
