LOCUS       sequence-272485-       12520 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Site specific insertion using CRISPR/Cas9 editing of C. elegans 
            ChrIV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12520)
  AUTHORS   Andrusiak MG, Sharifnia P, Lyu X, Wang Z, Dickey AM, Wu Z, Chisholm 
            AD, Jin Y
  TITLE     Inhibition of Axon Regeneration by Liquid-like TIAR-2 Granules.
  JOURNAL   Neuron. 2019 Jul 24. pii: S0896-6273(19)30602-6. doi: 
            10.1016/j.neuron.2019.07.004.
  PUBMED    31378567
REFERENCE   2  (bases 1 to 12520)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2019 Jul 24. pii: S0896-6273(19)30602-6. doi: 
            10.1016/j.neuron.2019.07.004."
COMMENT     Sequence Label: pCZGY2729
FEATURES             Location/Qualifiers
     source          1..12520
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(4..10,11..28,29..34))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    49..70
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(131..719)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(211..230)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      830..1339
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(917..936)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1123..1144
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(1381..2172,2173..2241))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2004..2023
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2242..2346)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2552..2574
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2566..2583
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2567..2583
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4278..4311
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    9116..9149
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    9179..9303
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(9344..9649)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(9446..9465)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(9991..10650)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     10565..10584
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(10704..10710,10711..10728,10729..10734))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(10759..10883)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(10899..10915)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10900..10916)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(12476..12492)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12476..12492)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12489..12511)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12500..12516
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
       61 actcacatta catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
      121 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
      181 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
      241 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
      301 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
      361 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
      421 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
      481 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
      541 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
      601 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
      661 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
      721 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
      781 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta cgcgccctgt
      841 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
      901 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgctttc
      961 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     1021 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga
     1081 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     1141 ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg attttgccga
     1201 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     1261 aaatattaac gtttacaatt taaatatttg cttatacaat cttcctgttt ttggggcttt
     1321 tctgattatc aaccggggta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     1381 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     1441 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     1501 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     1561 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     1621 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     1681 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     1741 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     1801 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     1861 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     1921 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     1981 ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct
     2041 catcattggg aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     2101 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     2161 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     2221 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     2281 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     2341 tccgcgcaca tttccccgaa aagtgccacc tgacgtagtt aacaaaaaaa agcccgccga
     2401 agcgggcttt attaccaagc gaagcgccat tcgccattca ggctgcgcaa ctgttgggaa
     2461 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
     2521 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
     2581 agtccgtaat acgactcact taaggccttg actagagggt acccatagcc tgcaggattc
     2641 catatttgaa gcggcacaat atgcatcatt catactgttg atggatgcct gtgtggcggt
     2701 cctctattga tttttgctgt ttttatataa tacttcttaa attgttcaaa tgaactagct
     2761 ttgctaatgt aagtaataaa ttatcaattc atgctacaac cttattatct gacttttcag
     2821 gcagccatgt acattgtgat tgggactttt gggaattgat aaattactca attgagttca
     2881 aaaggtaatt acaacttttt ccacatgaaa agtaattttc cgatattcac aggttacatc
     2941 aagggctacc cggtcaacaa caacgagaag ttcctgtaca gatttgagtt ggagccaaat
     3001 gccatagtca atgggcccca aaactccaaa cacaccagtc acaaaagaca atgttactca
     3061 gtttagccca atatttatct atgttttttg ttttgttttt tatgcaaata aaatttctgt
     3121 tacactataa attgttataa aatcagaatc ttacttccga atcagttttg ggaacacaaa
     3181 gatagaaacg caaagaagat ccaactatca acaatgttta ctagaccggg gctcgccatg
     3241 tttggcccat taggtcagac aaaaatttct gatctttaaa ccttattgat taaaactaat
     3301 ttttgcccga cttttttgac acatgcacta gacgcttgta ctcaaaattt ggaaaccttg
     3361 ctcaaatcca ctactcagat atgacagtag catcgataat tgaaccatta tcctgaaatc
     3421 ccctttcctg tcccggttca ttttccaatt ttttcaacgg tggaagaagg ggcaaatttt
     3481 ataccaaaac gtctccgttt atgacccacc ccctatgcaa aataaacggc tctgggagaa
     3541 atagtacagc aaacgcggag cctgcttgat tccttctccg aggcgacaaa aaacgcatca
     3601 ttcgtattgt aagatgcatg tgtggctggc ctcttttgaa ttttactggt tttttatatt
     3661 tctactaata tttcgaaaaa ttttgtaagt aaatcattga ttacgtttac aaaaatttag
     3721 aaacatttca aaatgattac ttctaaatta attatattta caggttagtt cgagttactt
     3781 atgaccttcc aatccgccat atccgccaag gacgctccaa ttaatttttt agaattattt
     3841 tttaacacat gtattatttt ttaacaataa attattcatg aaatcatcaa atgtatatgt
     3901 tctactataa tatttaggtc aaactataga aaagaaatcc ttgtttttgg aacaaaggag
     3961 ttcagatcct gtgcatttga tcttggatcc acatctttgg tccatgggaa agcttggcac
     4021 tgatcaagaa ggattcttca aatatctaaa attgccggct gcccgtagaa ttccatcagt
     4081 tttccagagt tttagtgaaa atttgcatcc cgttagaaac aatctacaca acggtaaaaa
     4141 ggacccatta tttttaccgt accgttttcc tttatggccc tctatgcaaa aaattatagc
     4201 gtggaggggc agtacagaat aataaagtgt gagaaaaaaa actgctctgc gagaaatagt
     4261 acagcaaacg cggagcagaa gttcctattc tctagaaagt ataggaactt cgggtgaaag
     4321 gaacgacact atgtacatca accagaaatt acaagatatt ggagaaatat attcaaataa
     4381 ataataaata catcattgtt acaatggagg gcgttttgtt gccatatcaa ccatttcgtt
     4441 atgttgtcga attggttgtg aatcccgtgg ctgaaaaaat tcttaaggtt attaaaacat
     4501 ttatcatcgc ataatacctt tggctgaata tcagaaattc cgtttcgact atgttttgga
     4561 ggactcgtac caacggggac agttttgatg gacatcaatg ttttactgag ttccaaaaga
     4621 ttttgagaaa gtactgcgca tagctcacta gatttcgcat ttcgaacatc ctcggcatat
     4681 atttctgaaa aatgattttg gaattcaaag aatttgtaac attatattgg ctaaaagatt
     4741 tgaaggttga taatatccat tacctaatgc cttagaaact tgacaacaca tcatatcctg
     4801 ttcattcagt cgagtctgga gagttgaaac ctgctcttcc atcatttgta cttcatttgc
     4861 ccagttatct ttcttttggg gagtcctcaa tggttctctt agcggctcat ttgatctccg
     4921 tggtgaagat ggacccgaaa cggtgttcga acgatcacga attgtgttct tcactttttg
     4981 aaatactgga gcaagcgcga gaatggttga gggtgactct gcgccatcat tctttgaatt
     5041 aactgctgct ggaggtcttt gaggacgttg agttctgttt ggatgactgc ttccagcaac
     5101 cgtgatatgt gaaaagttgt ttgattttat cattggtttt gcatttttct gaaaaaaata
     5161 ttatttttta tgactatgtt tcatttaata tactttagga ttcctatagt gtctggctaa
     5221 tttcacgaat gagtcgatat ctgctatcgg aggatatctc atgagatatt gaagacatcc
     5281 accgtagtct gatgacaata ctgtaaagaa gctttgtctt caaatttttt atcgatttaa
     5341 caacttacaa agatgtctga tctgaactaa caacgaaaca aacatgcatt tggctaatgg
     5401 ggcaattggc cgatcaatca gaagtacatc ccatagaaaa agcaaatcgt gtagaggaag
     5461 ttctcggcca aataataagc gaagccaacg actgtaaata ttttaattaa atattttcaa
     5521 aaataaaata actcacattc cataaagttg tggtggtata tcaagagtac taagatgttt
     5581 ggcaagggtg ggatcgattt cgtgtagaag atttccaata tcaattagat cttccatcaa
     5641 acgagatgca ggagcggaat cttgcaccct catgtacggt tcctttggaa ttggacgctt
     5701 tggactctca gtgactgttg gttcttcata ccatcttgat acttctaaca tcaccgaaca
     5761 aaacagatta ctaaaaatat attatcaata atgattgaaa tttattcact cactagctat
     5821 cctgttcgag gtattcttta caaaataagc aattcaaaat atcttcttct tccaccgtta
     5881 gcattttcaa ctcatcattc tcttttgcat gttgaaatgc ttcattgtcc gaatatatca
     5941 cgaaaattag cggtgccaga atttcgtgca ttccttgacg ataatttaca aaaggatgct
     6001 cctttgcata aaccaaaaga atatctgaca tcatttgccg aatacttgtg ttttgaaaga
     6061 actcaatttc cggaaaagtt cgtgaaacat ctttgccgat aatgtcacga agatcattgt
     6121 cctcgaaaaa tgtattccat ggattttgct caatagaggc aagtggattg ttgaattcag
     6181 gatcttgaga aaactttgta tcgtgtgggt caattaaatg attttccttg tgagctcggt
     6241 aaagattccg actacgtgat aagcttattt cccaatcact tgtttcatac ggcaaacatc
     6301 gaagaacaag ctgtaatcaa ttgacttttg aagactaatt tagaaatgcc gaaactaata
     6361 ccctccaaac agctgatcta catgaagatg aacgcaagga gcctgatgca gcgactcttg
     6421 ctaggagatc atcagaaagc ttatatttga ttagtgacgt cgcatcaaag ctgaacacat
     6481 aaattcttta atattttata tccccatatt atgcacctga gaggaatgaa gaaatttgct
     6541 gatactcccg gtgaattcat gtcttgatgg ttgtgcgcat tgtggtaata tctgaaaaat
     6601 ttcgaagtca gttttctgat aaggcaacaa ctaataaatt gagaatcaat ttacccttaa
     6661 ggaacgatgt tcgcttctga atttattaat aaatttttcc gtaattcaat tcaaaattgt
     6721 catatttaag aaaaaaacat caaaaatgac aaattcaata ttttaattaa tttttgcttt
     6781 cgtcgtaaat ctacacacgc gtctcttccg tgcgagagtc caagccagca gccaaattcg
     6841 ttgactgagt attcaacgtt tatacgttgt cggcaacgag aaataggaaa atgcatcggg
     6901 aaatgttctt ttttcgattt tttccaaggt tttgacaaat tttaccacga attttgctat
     6961 gttttcaatt aaaaaatatg ttattcaact gtttctatga ggaaaataag gctttgcatg
     7021 taattttctt attcagcata atttttaatt aatttgaatt ttctgtccta acgtttattt
     7081 tgttttcttg gttatgactg atctgaaatt aatttttgaa ttttaaggta atatgaaaaa
     7141 acccgagttg accgccacat ccgtagagaa gttcctcatc gagaagttcg actccgtctc
     7201 cgacctcatg caactctccg agggagagga gtcccgtgcc ttctccttcg acgtcggagg
     7261 acgtggatac gtcctccgtg tcaactcctg cgccgacgga ttctacaagg taagtttaaa
     7321 catatatata ctaactaacc ctgattattt aaattttcag gaccgttacg tctaccgtca
     7381 cttcgcctcc gccgccctcc caatcccaga ggtcctcgac atcggagagt tctccgagtc
     7441 cctcacctac tgcatctccc gtcgtgccca aggagtcacc ctccaagacc tcccagagac
     7501 cgagctccca gccgtcctcc aaccagtcgc cgaggccatg gacgccatcg ccgccgccga
     7561 cctctcccaa acctccggat tcggaccatt cggaccacaa ggaatcggac aatacaccac
     7621 ctggcgtgac ttcatctgcg ccatcgccga cccacacgtc taccactggc aaaccgtcat
     7681 ggacgacacc gtctccgcct ccgtcgccca agccctcgac gagctcatgc tctgggccga
     7741 ggactgccca gaggtccgtc acctcgtcca cgccgacttc ggatccaaca acgtcctcac
     7801 cgacaacgga cgtatcaccg ccgtcatcga ctggtccgag gccatgttcg gagactccca
     7861 atacgaggtc gccaacatct tcttctggcg tccatggctc gcctgcatgg agcaacaaac
     7921 ccgttacttc gagcgtcgtc acccagagct cgccggatcc ccacgtctcc gtgcctacat
     7981 gctccgtatc ggactcgacc aactctacca atccctcgtc gacggaaact tcgacgacgc
     8041 cgcctgggcc caaggacgtt gcgacgccat cgtccgttcc ggagccggaa ccgtcggacg
     8101 tacccaaatc gcccgtcgtt ccgccgccgt ctggaccgac ggatgcgtcg aggtcctcgc
     8161 cgactccgga aaccgtcgtc catccacccg tccacgtgcc aaggtaagtt taaacagttc
     8221 ggtactaact aaccatacat atttaaattt tcaggagtaa aattccaact gagcgccggt
     8281 cgctaccatt accaacttgt ctggtgtcaa aaataatagg ggccgctgtc atcagagtaa
     8341 gtttaaactg agttctacta actaacgagt aatatttaaa ttttcagcat ctcgcgcccg
     8401 tgcctctgac ttctaagtcc aattactctt caacatccct acatgctctt tctccctgtg
     8461 ctcccacccc ctatttttgt tattatcaaa aaacttctct taatttcttt gttttttagc
     8521 ttcttttaag tcacctctaa caatgaaatt gtgtagattc aaaaatagaa ttaattcgta
     8581 ataaaaagtc gaaaaaaatt gtgctccctc cccccattaa taataattct atcccaaaat
     8641 ctacacaatg ttctgtgtac acttcttatg ttttttactt ctgataaatt tttttgaaac
     8701 atcatagaaa aaaccgcaca caaaatacct tatcatatgt tacgtttcag tttatgaccg
     8761 caatttttat ttcttcgcac gtctgggcct ctcatgacgt caaatcatgc tcatcgtgaa
     8821 aaagttttgg agtatttttg gaatttttca atcaagtgaa agtttatgaa attaattttc
     8881 ctgcttttgc tttttggggt ttcccctatt gtttgtcaag atttcgagga cggcgttttt
     8941 cttgctaaaa tcacaagtat tgatgagcac gatgcaagaa agatcggaag aaggtttggg
     9001 tttgaggctc agtggaaggt gagtagaagt tgataatttg aaagtggagt agtgtctatg
     9061 gggtttttgc cttaaatgac agaatacatt cccaatatac caaacataac tgtttgaagt
     9121 tcctattctc tagaaagtat aggaacttcg catgccgggc tgcaggaatt cgatatcaac
     9181 cactttgtac aagaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa
     9241 ttagattttg cataaaaaac agactacata atactgtaaa acacaacata tccagtcact
     9301 atggtcgacc tgcagactgg ctgtgtataa gggagcctga catttatatt ccccagaaca
     9361 tcaggttaat ggcgtttttg atgtcatttt cgcggtggct gagatcagcc acttcttccc
     9421 cgataacgga gaccggcaca ctggccatat cggtggtcat catgcgccag ctttcatccc
     9481 cgatatgcac caccgggtaa agttcacggg agactttatc tgacagcaga cgtgcactgg
     9541 ccagggggat caccatccgt cgcccgggcg tgtcaataat atcactctgt acatccacaa
     9601 acagacgata acggctctct cttttatagg tgtaaacctt aaactgcatt tcaccagccc
     9661 ctgttctcgt cagcaaaaga gccgttcatt tcaataaacc gggcgacctc agccatccct
     9721 tcctgatttt ccgctttcca gcgttcggca cgcagacgac gggcttcatt ctgcatggtt
     9781 gtgcttacca gaccggagat attgacatca tatatgcctt gagcaactga tagctgtcgc
     9841 tgtcaactgt cactgtaata cgctgcttca tagcatacct ctttttgaca tacttcgggt
     9901 atacatatca gtatatattc ttataccgca aaaatcagcg cgcaaatacg catactgtta
     9961 tctggctttt agtaagccgg atccagatct ttacgccccg ccctgccact catcgcagta
    10021 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac
    10081 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa
    10141 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac
    10201 ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag
    10261 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc
    10321 gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca
    10381 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac ggaattccgg
    10441 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt
    10501 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca
    10561 ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac
    10621 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
    10681 cggatcctaa ctcaaaatcc acacattata cgagccggaa gcataaagtg taaagcctgg
    10741 ggtgcctaat gcggccgcca tagtgactgg atatgttgtg ttttacagta ttatgtagtc
    10801 tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt
    10861 tcagcttttt tgtacaaact tgttgatatc aagcttatcg ataccgtcga cctcgagacg
    10921 tacggtgcgc gcgatgcatt cgaagatctg cccactagtg tgtagcggtc ctctattgat
    10981 ttttgctatt ttttgataat tttactgtaa attatgagtt attccaatta acattcctag
    11041 ctaaatgtaa gttagcgacc aattttttag caaccccatt ttatgacttt tcagaatatc
    11101 gcctactggg tcaacgacac ttgtttggaa ctaagggtct tcccaatttt ttttaaatct
    11161 tcataggctt gacagaatca gggttcttca agcagctttg agagggaatc aattgcaaca
    11221 tttgatgtta aatttatatt ttttatagtt ctctggtgaa atggggacca ccgcaaagcc
    11281 ccttttatgt ttttaacttg aataaaatct tgtattaaat tgtgatttat agatctgtat
    11341 gggtaatttg aaggaaaaga aagggagctc ccaaaagtgc cttacagatc caggcccaat
    11401 aaatgtaaac tattgggcag tagtatggca aaaatcgtgc ggaatttgaa aaacctagaa
    11461 ttaaaataaa tattcaacac aaatgttcga gatattttaa gttcggagtg tatatctaga
    11521 acatattgta caagtaggtc tacgggatcg tctacgggaa aatttgattt cctcgaagat
    11581 gtatttaatg aattctctaa tgttgcaggt ctttgtattt gacagaatgt tggtttttga
    11641 aacttattcg ccataattgc ttggctacta catgtctcat gccattgaca ctatcctgta
    11701 ctcgaaattt gttaagtaac tacccaccgt ccacttatga cgagagggtc aattaaggtc
    11761 actccacaaa agtaaatttg aaaacacatg agaaaaagtg atctgaattc ccttgaagtg
    11821 gctaaaacaa agcagatgag ggatacgacg tatgcgacta ctgagagata aggtcgaaac
    11881 ttgataagga tgctgcttac ctgcttagca agtttcttcg cacaggagaa agtgacaagc
    11941 taaactatca aattcatcta aaacctcaaa aaattatttt ggatgaaata atgttttatt
    12001 ttgagcaacg cataaggcaa agaataaatt aaaggcgatc cgcttagcgg cgagttccgt
    12061 cctcgaagaa gattcctcca tcgatggcgc agtactttgg acagattcca cgctctcctg
    12121 gggttcctgg tggtccgact ggtcctggct gtcctgggtt tccgtcggct cctggcttgc
    12181 cgtctggtcc tggtggtccc tttggtccag gttgtcctga aagatgtggt gattagatat
    12241 ggattgaaaa actatgaaat gattacctgg ggttccgtcg tttcctggtt gtccggatgg
    12301 tcctggtggt ccgactggtc ctggctctcc ggtggatcct ggctctccct tttggactgg
    12361 ctcgcagaca gctggaagtc cctcgtctcc tggtgctcct ggtgctcctg gctttcctgg
    12421 tgctccgttt ggtccctttg gtccttgctc tcctgcgtag cttggcgtaa tcatggtcat
    12481 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
//
