LOCUS       sequence-272489-        5517 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression of  CENP-T and CENP-W.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5517)
  AUTHORS   McKinley KL, Sekulic N, Guo LY, Tsinman T, Black BE, Cheeseman IM
  TITLE     The CENP-L-N Complex Forms a Critical Node in an Integrated Meshwork
            of Interactions at the Centromere-Kinetochore Interface.
  JOURNAL   Mol Cell. 2015 Dec 17;60(6):886-98. doi: 
            10.1016/j.molcel.2015.10.027. Epub 2015 Nov 19.
  PUBMED    26698661
REFERENCE   2  (bases 1 to 5517)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.10.027"; journalName: "Mol Cell"; date: 
            "2015-12-17- 17"; volume: "60"; issue: "6"; pages: "886-98"
COMMENT     Sequence Label: pKM498
FEATURES             Location/Qualifiers
     source          1..5517
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(90..95,96..112,113..118))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(189..222)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        299..390
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             436..456
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     polyA_signal    2355..2489
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2661..2752
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    3262..3396
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  3517..3682
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3872..4460
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4361..4380
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  4595..4818
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4885..5418)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt
       61 tgcgtgagcg catacgctac ttgcattaca gtttacgaac cgaacaggct tatgtcaact
      121 gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc ggcaaccttg ggcagcagcg
      181 aagtcgccat aacttcgtat agcatacatt atacgaagtt atctgtaact ataacggtcc
      241 taaggtagcg agtttaaaca ctagtatcga ttcgcgacct actccggaat attaatagat
      301 catggagata attaaaatga taaccatctc gcaaataaat aagtatttta ctgttttcgt
      361 aacagttttg taataaaaaa acctataaat attccggatt attcataccg tcccaccatc
      421 gggcgcggat ccatgcatca tcatcatcat catcatggca gcggcagcat ggcagaccac
      481 aaccctgaca gcgattctac acctcgtaca ctgctgcgtc gtgtgctgga tactgctgat
      541 cctcgtactc ctcgtcgtcc tcgtagcgct cgtgctggtg ctcgccgtgc cctgctggaa
      601 acagcctctc ctcgtaaact gagtggccag acccgtacta ttgcccgtgg gcgttcacat
      661 ggagcccgct cggtcggtcg tagtgctcac attcaagcct cgggacacct ggaagaacaa
      721 acccctcgta ccctgctgaa aaacatcctg ctgactgccc ctgaaagctc tattctgatg
      781 ccggaaagcg tcgttaaacc ggttcctgct cctcaagccg ttcaaccttc tcgtcaagag
      841 tcctcctgtg gatctctgga gctgcaactg cctgaactgg agcctcctac aacactggca
      901 ccggggctgc tggcgcctgg tcgtcgtaaa caacgtctgc gtctgagcgt gtttcaacaa
      961 ggtgttgatc agggcctgag tctgtctcaa gaacctcaag gcaacgctga tgcttctagt
     1021 ctgacccgtt ctctgaacct gacctttgct actccactgc aacctcaaag cgtgcaacgt
     1081 cctggactgg ctcgccgccc tcctgctcgc cgtgccgttg atgtgggtgc ttttctgcgt
     1141 gacctgcgtg atacaagcct ggcacctccg aatattgtgc tggaggatac acagccgttt
     1201 tcacagccta tggttggtag ccctaacgtt tatcactcac tgccgtgtac ccctcacact
     1261 ggtgccgaag atgctgaaca agctgctggt cgtaaaacac aaagctctgg accgggactg
     1321 caaaaaaact ctccggggaa acctgcccaa ttcctggcgg gtgaagctga agaagtcaac
     1381 gcctttgctc tgggatttct gtctaccagc tcgggtgtta gcggagaaga tgaggtggaa
     1441 cctctgcatg atggtgtgga agaagccgag aagaaaatgg aagaggaagg ggtgtctgtc
     1501 tctgaaatgg aagcgactgg tgctcaagga ccaagtcgtg tcgaagaggc agaaggccat
     1561 accgaggtta ccgaagccga aggaagccaa gggacagctg aagctgatgg acctggggcc
     1621 tcaagtgggg atgaagatgc tagtggccgt gccgcctcac ctgaatctgc ttctagtaca
     1681 ccggaaagcc tgcaagcacg tcgtcatcac caatttctgg aacctgctcc ggctcctggt
     1741 gctgctgttc tgtctagtga acctgctgaa cctctgctgg ttcgtcatcc gcctcgtcct
     1801 cgtacaactg gaccacgtcc tcgccaagac cctcataaag caggactgag ccattatgtt
     1861 aaactgttct cgttctatgc caaaatgccg atggagcgta aagctctgga aatggtcgaa
     1921 aaatgcctgg acaaatattt ccaacatctg tgtgacgatc tggaagtgtt tgccgctcat
     1981 gctgggcgta aaacagtgaa acctgaggat ctggagctgc tgatgcgtcg tcaaggactg
     2041 gttacggatc aagtgtcgct gcatgtcctg gtggaacgtc atctgccgct ggaatatcgc
     2101 caactgctga ttccttgtgc ttatagcggg aactccgtct ttcctgccca gtaagaattc
     2161 aaaggcctac gtcgacgagc tcacttgtcg cggccgcttt cgaatctaga gcctgcagtc
     2221 tcgacaagct tgtcgagaag tactagagga tcataatcag ccataccaca tttgtagagg
     2281 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     2341 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     2401 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     2461 tcatcaatgt atcttatcat gtctggatct gatcactgct tgagcctaga agatccggct
     2521 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactatca
     2581 taacccctag ggtataccca tctaaggtag cgagtttaaa cactagtatc gattcgcgac
     2641 ctactccgga atattaatag atcatggaga taattaaaat gataaccatc tcgcaaataa
     2701 ataagtattt tactgttttc gtaacagttt tgtaataaaa aaacctataa atattccgga
     2761 ttattcatac cgtcccacca tcgggcgcgg atccatggca ctgtctacga ttgtgagtca
     2821 gcgtaaacaa atcaaacgta aagcccctcg tggtttcctg aaacgtgtgt ttaaacgtaa
     2881 aaaacctcag ctgcgcctgg aaaaatcagg tgatctgctg gttcatctga attgcctgtt
     2941 attcgtccac cgtctggcag aagaatcccg tactaatgcc tgcgcttcga aatgtcgtgt
     3001 catcaacaaa gaacatgtgc tggctgccgc taaagtcatt ctgaaaaaat cccgtggctg
     3061 agaattcaaa ggcctacgtc gacgagctca cttgtcgcgg ccgctttcga atctagagcc
     3121 tgcagtctcg acaagcttgt cgagaagtac tagaggatca taatcagcca taccacattt
     3181 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     3241 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     3301 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     3361 tccaaactca tcaatgtatc ttatcatgtc tggatctgat cactgcttga gcctagaaga
     3421 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
     3481 actatcataa cccctagggt atacccatct aattggaacc agataagtga aatctagttc
     3541 caaactattt tgtcattttt aattttcgta ttagcttacg acgctacacc cagttcccat
     3601 ctattttgtc actcttccct aaataatcct taaaaactcc atttccaccc ctcccagttc
     3661 ccaactattt tgtccgccca caaccggttg acttgggtca actgtcagac caagtttact
     3721 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     3781 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     3841 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     3901 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     3961 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     4021 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     4081 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     4141 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     4201 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     4261 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     4321 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     4381 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     4441 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     4501 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattgactt gggtcgctct
     4561 tcctgtggat gcgcagatgc cctgcgtaag cgggtgtggg cggacaataa agtcttaaac
     4621 tgaacaaaat agatctaaac tatgacaata aagtcttaaa ctagacagaa tagttgtaaa
     4681 ctgaaatcag tccagttatg ctgtgaaaaa gcatactgga cttttgttat ggctaaagca
     4741 aactcttcat tttctgaagt gcaaattgcc cgtcgtatta aagaggggcg tggccaaggg
     4801 catgtaaaga ctatattcgc ggcgttgtga caatttaccg aacaactccg cggccgggaa
     4861 gccgatctcg gcttgaacga attgttaggt ggcggtactt gggtcgatat caaagtgcat
     4921 cacttcttcc cgtatgccca actttgtata gagagccact gcgggatcgt caccgtaatc
     4981 tgcttgcacg tagatcacat aagcaccaag cgcgttggcc tcatgcttga ggagattgat
     5041 gagcgcggtg gcaatgccct gcctccggtg ctcgccggag actgcgagat catagatata
     5101 gatctcacta cgcggctgct caaacttggg cagaacgtaa gccgcgagag cgccaacaac
     5161 cgcttcttgg tcgaaggcag caagcgcgat gaatgtctta ctacggagca agttcccgag
     5221 gtaatcggag tccggctgat gttgggagta ggtggctacg tctccgaact cacgaccgaa
     5281 aagatcaaga gcagcccgca tggatttgac ttggtcaggg ccgagcctac atgtgcgaat
     5341 gatgcccata cttgagccac ctaactttgt tttagggcga ctgccctgct gcgtaacatc
     5401 gttgctgctg cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc acggcgtaac
     5461 gcgcttgctg cttggatgcc cgaggcatag actgtacaaa aaaacagtca taacaag
//
